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Phevamine A, a small molecule that suppresses plant immune responses

Bacterial plant pathogens cause significant crop damage worldwide. They invade plant cells by producing a variety of virulence factors, including small-molecule toxins and phytohormone mimics. Virulence of the model pathogen Pseudomonas syringae pv. tomato DC3000 (Pto) is regulated in part by the si...

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Autores principales: O’Neill, Erinn M., Mucyn, Tatiana S., Patteson, Jon B., Finkel, Omri M., Chung, Eui-Hwan, Baccile, Joshua A., Massolo, Elisabetta, Schroeder, Frank C., Dangl, Jeffery L., Li, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187163/
https://www.ncbi.nlm.nih.gov/pubmed/30237288
http://dx.doi.org/10.1073/pnas.1803779115
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author O’Neill, Erinn M.
Mucyn, Tatiana S.
Patteson, Jon B.
Finkel, Omri M.
Chung, Eui-Hwan
Baccile, Joshua A.
Massolo, Elisabetta
Schroeder, Frank C.
Dangl, Jeffery L.
Li, Bo
author_facet O’Neill, Erinn M.
Mucyn, Tatiana S.
Patteson, Jon B.
Finkel, Omri M.
Chung, Eui-Hwan
Baccile, Joshua A.
Massolo, Elisabetta
Schroeder, Frank C.
Dangl, Jeffery L.
Li, Bo
author_sort O’Neill, Erinn M.
collection PubMed
description Bacterial plant pathogens cause significant crop damage worldwide. They invade plant cells by producing a variety of virulence factors, including small-molecule toxins and phytohormone mimics. Virulence of the model pathogen Pseudomonas syringae pv. tomato DC3000 (Pto) is regulated in part by the sigma factor HrpL. Our study of the HrpL regulon identified an uncharacterized, three-gene operon in Pto that is controlled by HrpL and related to the Erwinia hrp-associated systemic virulence (hsv) operon. Here, we demonstrate that the hsv operon contributes to the virulence of Pto on Arabidopsis thaliana and suppresses bacteria-induced immune responses. We show that the hsv-encoded enzymes in Pto synthesize a small molecule, phevamine A. This molecule consists of l-phenylalanine, l-valine, and a modified spermidine, and is different from known small molecules produced by phytopathogens. We show that phevamine A suppresses a potentiation effect of spermidine and l-arginine on the reactive oxygen species burst generated upon recognition of bacterial flagellin. The hsv operon is found in the genomes of divergent bacterial genera, including ∼37% of P. syringae genomes, suggesting that phevamine A is a widely distributed virulence factor in phytopathogens. Our work identifies a small-molecule virulence factor and reveals a mechanism by which bacterial pathogens overcome plant defense. This work highlights the power of omics approaches in identifying important small molecules in bacteria–host interactions.
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spelling pubmed-61871632018-10-15 Phevamine A, a small molecule that suppresses plant immune responses O’Neill, Erinn M. Mucyn, Tatiana S. Patteson, Jon B. Finkel, Omri M. Chung, Eui-Hwan Baccile, Joshua A. Massolo, Elisabetta Schroeder, Frank C. Dangl, Jeffery L. Li, Bo Proc Natl Acad Sci U S A PNAS Plus Bacterial plant pathogens cause significant crop damage worldwide. They invade plant cells by producing a variety of virulence factors, including small-molecule toxins and phytohormone mimics. Virulence of the model pathogen Pseudomonas syringae pv. tomato DC3000 (Pto) is regulated in part by the sigma factor HrpL. Our study of the HrpL regulon identified an uncharacterized, three-gene operon in Pto that is controlled by HrpL and related to the Erwinia hrp-associated systemic virulence (hsv) operon. Here, we demonstrate that the hsv operon contributes to the virulence of Pto on Arabidopsis thaliana and suppresses bacteria-induced immune responses. We show that the hsv-encoded enzymes in Pto synthesize a small molecule, phevamine A. This molecule consists of l-phenylalanine, l-valine, and a modified spermidine, and is different from known small molecules produced by phytopathogens. We show that phevamine A suppresses a potentiation effect of spermidine and l-arginine on the reactive oxygen species burst generated upon recognition of bacterial flagellin. The hsv operon is found in the genomes of divergent bacterial genera, including ∼37% of P. syringae genomes, suggesting that phevamine A is a widely distributed virulence factor in phytopathogens. Our work identifies a small-molecule virulence factor and reveals a mechanism by which bacterial pathogens overcome plant defense. This work highlights the power of omics approaches in identifying important small molecules in bacteria–host interactions. National Academy of Sciences 2018-10-09 2018-09-20 /pmc/articles/PMC6187163/ /pubmed/30237288 http://dx.doi.org/10.1073/pnas.1803779115 Text en Copyright © 2018 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle PNAS Plus
O’Neill, Erinn M.
Mucyn, Tatiana S.
Patteson, Jon B.
Finkel, Omri M.
Chung, Eui-Hwan
Baccile, Joshua A.
Massolo, Elisabetta
Schroeder, Frank C.
Dangl, Jeffery L.
Li, Bo
Phevamine A, a small molecule that suppresses plant immune responses
title Phevamine A, a small molecule that suppresses plant immune responses
title_full Phevamine A, a small molecule that suppresses plant immune responses
title_fullStr Phevamine A, a small molecule that suppresses plant immune responses
title_full_unstemmed Phevamine A, a small molecule that suppresses plant immune responses
title_short Phevamine A, a small molecule that suppresses plant immune responses
title_sort phevamine a, a small molecule that suppresses plant immune responses
topic PNAS Plus
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187163/
https://www.ncbi.nlm.nih.gov/pubmed/30237288
http://dx.doi.org/10.1073/pnas.1803779115
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