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Dielectrophoretic Microfluidic Device for in Vitro Fertilization
The aim of this work was to create a microfluidic platform that uses in vitro fertilization (IVF) and avoids unnecessary damage to oocytes due to the dielectrophoretic force manipulation of the sperms and oocytes that occurs in a traditional IVF operation. The device from this research can serve als...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187277/ https://www.ncbi.nlm.nih.gov/pubmed/30424069 http://dx.doi.org/10.3390/mi9030135 |
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author | Huang, Hong-Yuan Lai, Yun-Li Yao, Da-Jeng |
author_facet | Huang, Hong-Yuan Lai, Yun-Li Yao, Da-Jeng |
author_sort | Huang, Hong-Yuan |
collection | PubMed |
description | The aim of this work was to create a microfluidic platform that uses in vitro fertilization (IVF) and avoids unnecessary damage to oocytes due to the dielectrophoretic force manipulation of the sperms and oocytes that occurs in a traditional IVF operation. The device from this research can serve also to decrease medium volumes, as well as the cost of cell culture under evaporation, and to prevent unnecessary risk in intracytoplasmic sperm injection (ICSI). To decrease the impact and destruction of the oocyte and the sperm, we adopted a positive dielectrophoretic force to manipulate both the sperms and the oocyte. The mouse oocytes were trapped with a positive dielectrophoretic (p-DEP) force by using Indium Tin Oxide (ITO)-glass electrodes; the ITO-glass electrode chip was fabricated by wet etching the ITO-glass. The polydimethylsiloxane (PDMS) flow-focusing microfluidic device was used to generate microdroplets of micrometer size to contain the zygotes. The volume of the microdroplets was controlled by adjusting the flow rates of both inlets for oil and the DEP buffer. As a result, the rate of fertilization was increased by about 5% beyond that of the DEP treatment in traditional IVF, and more than 20% developed to the blastocyst stage with a low sperm-oocyte ratio. |
format | Online Article Text |
id | pubmed-6187277 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61872772018-11-01 Dielectrophoretic Microfluidic Device for in Vitro Fertilization Huang, Hong-Yuan Lai, Yun-Li Yao, Da-Jeng Micromachines (Basel) Article The aim of this work was to create a microfluidic platform that uses in vitro fertilization (IVF) and avoids unnecessary damage to oocytes due to the dielectrophoretic force manipulation of the sperms and oocytes that occurs in a traditional IVF operation. The device from this research can serve also to decrease medium volumes, as well as the cost of cell culture under evaporation, and to prevent unnecessary risk in intracytoplasmic sperm injection (ICSI). To decrease the impact and destruction of the oocyte and the sperm, we adopted a positive dielectrophoretic force to manipulate both the sperms and the oocyte. The mouse oocytes were trapped with a positive dielectrophoretic (p-DEP) force by using Indium Tin Oxide (ITO)-glass electrodes; the ITO-glass electrode chip was fabricated by wet etching the ITO-glass. The polydimethylsiloxane (PDMS) flow-focusing microfluidic device was used to generate microdroplets of micrometer size to contain the zygotes. The volume of the microdroplets was controlled by adjusting the flow rates of both inlets for oil and the DEP buffer. As a result, the rate of fertilization was increased by about 5% beyond that of the DEP treatment in traditional IVF, and more than 20% developed to the blastocyst stage with a low sperm-oocyte ratio. MDPI 2018-03-20 /pmc/articles/PMC6187277/ /pubmed/30424069 http://dx.doi.org/10.3390/mi9030135 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Huang, Hong-Yuan Lai, Yun-Li Yao, Da-Jeng Dielectrophoretic Microfluidic Device for in Vitro Fertilization |
title | Dielectrophoretic Microfluidic Device for in Vitro Fertilization |
title_full | Dielectrophoretic Microfluidic Device for in Vitro Fertilization |
title_fullStr | Dielectrophoretic Microfluidic Device for in Vitro Fertilization |
title_full_unstemmed | Dielectrophoretic Microfluidic Device for in Vitro Fertilization |
title_short | Dielectrophoretic Microfluidic Device for in Vitro Fertilization |
title_sort | dielectrophoretic microfluidic device for in vitro fertilization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187277/ https://www.ncbi.nlm.nih.gov/pubmed/30424069 http://dx.doi.org/10.3390/mi9030135 |
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