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Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array

Circulating tumor cells (CTCs) are regarded as a strong biomarker which includes clinically valuable information. However, CTCs are very rare and require precise separation and detection for effective clinical applications. Furthermore, downstream analysis has become necessary to identify the distin...

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Autores principales: Yoon, Yousang, Lee, Jusin, Yoo, Ki-Chun, Sul, Onejae, Lee, Su-Jae, Lee, Seung-Beck
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187321/
https://www.ncbi.nlm.nih.gov/pubmed/30424040
http://dx.doi.org/10.3390/mi9030106
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author Yoon, Yousang
Lee, Jusin
Yoo, Ki-Chun
Sul, Onejae
Lee, Su-Jae
Lee, Seung-Beck
author_facet Yoon, Yousang
Lee, Jusin
Yoo, Ki-Chun
Sul, Onejae
Lee, Su-Jae
Lee, Seung-Beck
author_sort Yoon, Yousang
collection PubMed
description Circulating tumor cells (CTCs) are regarded as a strong biomarker which includes clinically valuable information. However, CTCs are very rare and require precise separation and detection for effective clinical applications. Furthermore, downstream analysis has become necessary to identify the distinct sub-population of CTCs that causes metastasis. Here, we report a flow-restricted microfluidic trap array capable of deterministic single-cell capture of CTCs. The extent of flow restriction, correlating with the device geometry, was then optimized using a highly invasive breast cancer cell line (LM2 MDA-MB-231) to achieve 97% capture efficiency with a single-cell capture rate of 99%. Single-cell capture of CTCs from mice with full-blown metastasis was also demonstrated. The single-CTC capturing ability of the flow-restricted trap array not only showed cell enumerating ability but also high prospects for application in future automated downstream analysis.
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spelling pubmed-61873212018-11-01 Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array Yoon, Yousang Lee, Jusin Yoo, Ki-Chun Sul, Onejae Lee, Su-Jae Lee, Seung-Beck Micromachines (Basel) Article Circulating tumor cells (CTCs) are regarded as a strong biomarker which includes clinically valuable information. However, CTCs are very rare and require precise separation and detection for effective clinical applications. Furthermore, downstream analysis has become necessary to identify the distinct sub-population of CTCs that causes metastasis. Here, we report a flow-restricted microfluidic trap array capable of deterministic single-cell capture of CTCs. The extent of flow restriction, correlating with the device geometry, was then optimized using a highly invasive breast cancer cell line (LM2 MDA-MB-231) to achieve 97% capture efficiency with a single-cell capture rate of 99%. Single-cell capture of CTCs from mice with full-blown metastasis was also demonstrated. The single-CTC capturing ability of the flow-restricted trap array not only showed cell enumerating ability but also high prospects for application in future automated downstream analysis. MDPI 2018-03-02 /pmc/articles/PMC6187321/ /pubmed/30424040 http://dx.doi.org/10.3390/mi9030106 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yoon, Yousang
Lee, Jusin
Yoo, Ki-Chun
Sul, Onejae
Lee, Su-Jae
Lee, Seung-Beck
Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array
title Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array
title_full Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array
title_fullStr Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array
title_full_unstemmed Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array
title_short Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array
title_sort deterministic capture of individual circulating tumor cells using a flow-restricted microfluidic trap array
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187321/
https://www.ncbi.nlm.nih.gov/pubmed/30424040
http://dx.doi.org/10.3390/mi9030106
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