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Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array
Circulating tumor cells (CTCs) are regarded as a strong biomarker which includes clinically valuable information. However, CTCs are very rare and require precise separation and detection for effective clinical applications. Furthermore, downstream analysis has become necessary to identify the distin...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187321/ https://www.ncbi.nlm.nih.gov/pubmed/30424040 http://dx.doi.org/10.3390/mi9030106 |
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author | Yoon, Yousang Lee, Jusin Yoo, Ki-Chun Sul, Onejae Lee, Su-Jae Lee, Seung-Beck |
author_facet | Yoon, Yousang Lee, Jusin Yoo, Ki-Chun Sul, Onejae Lee, Su-Jae Lee, Seung-Beck |
author_sort | Yoon, Yousang |
collection | PubMed |
description | Circulating tumor cells (CTCs) are regarded as a strong biomarker which includes clinically valuable information. However, CTCs are very rare and require precise separation and detection for effective clinical applications. Furthermore, downstream analysis has become necessary to identify the distinct sub-population of CTCs that causes metastasis. Here, we report a flow-restricted microfluidic trap array capable of deterministic single-cell capture of CTCs. The extent of flow restriction, correlating with the device geometry, was then optimized using a highly invasive breast cancer cell line (LM2 MDA-MB-231) to achieve 97% capture efficiency with a single-cell capture rate of 99%. Single-cell capture of CTCs from mice with full-blown metastasis was also demonstrated. The single-CTC capturing ability of the flow-restricted trap array not only showed cell enumerating ability but also high prospects for application in future automated downstream analysis. |
format | Online Article Text |
id | pubmed-6187321 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61873212018-11-01 Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array Yoon, Yousang Lee, Jusin Yoo, Ki-Chun Sul, Onejae Lee, Su-Jae Lee, Seung-Beck Micromachines (Basel) Article Circulating tumor cells (CTCs) are regarded as a strong biomarker which includes clinically valuable information. However, CTCs are very rare and require precise separation and detection for effective clinical applications. Furthermore, downstream analysis has become necessary to identify the distinct sub-population of CTCs that causes metastasis. Here, we report a flow-restricted microfluidic trap array capable of deterministic single-cell capture of CTCs. The extent of flow restriction, correlating with the device geometry, was then optimized using a highly invasive breast cancer cell line (LM2 MDA-MB-231) to achieve 97% capture efficiency with a single-cell capture rate of 99%. Single-cell capture of CTCs from mice with full-blown metastasis was also demonstrated. The single-CTC capturing ability of the flow-restricted trap array not only showed cell enumerating ability but also high prospects for application in future automated downstream analysis. MDPI 2018-03-02 /pmc/articles/PMC6187321/ /pubmed/30424040 http://dx.doi.org/10.3390/mi9030106 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yoon, Yousang Lee, Jusin Yoo, Ki-Chun Sul, Onejae Lee, Su-Jae Lee, Seung-Beck Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array |
title | Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array |
title_full | Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array |
title_fullStr | Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array |
title_full_unstemmed | Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array |
title_short | Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array |
title_sort | deterministic capture of individual circulating tumor cells using a flow-restricted microfluidic trap array |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187321/ https://www.ncbi.nlm.nih.gov/pubmed/30424040 http://dx.doi.org/10.3390/mi9030106 |
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