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Continuous-Flow Cell Dipping and Medium Exchange in a Microdevice using Dielectrophoresis

Medium exchange is the process of changing the suspension medium of cells/particles, and has applications in washing, surface modifications, nutrient replenishment, or simply changing the environment of the target entities. Dipping involves diverting the path of target cells in the carrying fluid to...

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Autores principales: Alhammadi, Falah, Waheed, Waqas, El-Khasawneh, Bashar, Alazzam, Anas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187335/
https://www.ncbi.nlm.nih.gov/pubmed/30424156
http://dx.doi.org/10.3390/mi9050223
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author Alhammadi, Falah
Waheed, Waqas
El-Khasawneh, Bashar
Alazzam, Anas
author_facet Alhammadi, Falah
Waheed, Waqas
El-Khasawneh, Bashar
Alazzam, Anas
author_sort Alhammadi, Falah
collection PubMed
description Medium exchange is the process of changing the suspension medium of cells/particles, and has applications in washing, surface modifications, nutrient replenishment, or simply changing the environment of the target entities. Dipping involves diverting the path of target cells in the carrying fluid to immerse them in another fluid for a short duration, and pushing them again into the original medium. In this paper, a simple microfluidic platform is introduced that employs dielectrophoresis to achieve medium exchange and dipping of micro-objects in a continuous manner. The essential feature of the platform is a microchannel that includes two arrays of microelectrodes that partly enter the bottom surface from both sides. In the first step, numerous finite element-based parametric studies are carried out to obtain the optimized geometrical and operational parameters ensuring successful dipping and medium exchange processes. The results of those studies are utilized to fabricate the platform using standard photolithography techniques. The electrodes are patterned on a glass substrate, while the channel, made out of polydimethylsiloxane, is bonded on top of the glass. Trajectories of blood cells from numerical studies and experimentations are reported, and both results exhibited close agreement.
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spelling pubmed-61873352018-11-01 Continuous-Flow Cell Dipping and Medium Exchange in a Microdevice using Dielectrophoresis Alhammadi, Falah Waheed, Waqas El-Khasawneh, Bashar Alazzam, Anas Micromachines (Basel) Article Medium exchange is the process of changing the suspension medium of cells/particles, and has applications in washing, surface modifications, nutrient replenishment, or simply changing the environment of the target entities. Dipping involves diverting the path of target cells in the carrying fluid to immerse them in another fluid for a short duration, and pushing them again into the original medium. In this paper, a simple microfluidic platform is introduced that employs dielectrophoresis to achieve medium exchange and dipping of micro-objects in a continuous manner. The essential feature of the platform is a microchannel that includes two arrays of microelectrodes that partly enter the bottom surface from both sides. In the first step, numerous finite element-based parametric studies are carried out to obtain the optimized geometrical and operational parameters ensuring successful dipping and medium exchange processes. The results of those studies are utilized to fabricate the platform using standard photolithography techniques. The electrodes are patterned on a glass substrate, while the channel, made out of polydimethylsiloxane, is bonded on top of the glass. Trajectories of blood cells from numerical studies and experimentations are reported, and both results exhibited close agreement. MDPI 2018-05-08 /pmc/articles/PMC6187335/ /pubmed/30424156 http://dx.doi.org/10.3390/mi9050223 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alhammadi, Falah
Waheed, Waqas
El-Khasawneh, Bashar
Alazzam, Anas
Continuous-Flow Cell Dipping and Medium Exchange in a Microdevice using Dielectrophoresis
title Continuous-Flow Cell Dipping and Medium Exchange in a Microdevice using Dielectrophoresis
title_full Continuous-Flow Cell Dipping and Medium Exchange in a Microdevice using Dielectrophoresis
title_fullStr Continuous-Flow Cell Dipping and Medium Exchange in a Microdevice using Dielectrophoresis
title_full_unstemmed Continuous-Flow Cell Dipping and Medium Exchange in a Microdevice using Dielectrophoresis
title_short Continuous-Flow Cell Dipping and Medium Exchange in a Microdevice using Dielectrophoresis
title_sort continuous-flow cell dipping and medium exchange in a microdevice using dielectrophoresis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187335/
https://www.ncbi.nlm.nih.gov/pubmed/30424156
http://dx.doi.org/10.3390/mi9050223
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