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Long-Term Tracking of Free-Swimming Paramecium caudatum in Viscous Media Using a Curved Sample Chamber

It is technically difficult to acquire large-field images under the complexity and cost restrictions of a diagnostic and instant field research purpose. The goal of the introduced large-field imaging system is to achieve a tolerable resolution for detecting microscale particles or objects in the ent...

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Autores principales: Shourav, Mohiuddin Khan, Kim, Jung Kyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187482/
https://www.ncbi.nlm.nih.gov/pubmed/30393284
http://dx.doi.org/10.3390/mi9010007
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author Shourav, Mohiuddin Khan
Kim, Jung Kyung
author_facet Shourav, Mohiuddin Khan
Kim, Jung Kyung
author_sort Shourav, Mohiuddin Khan
collection PubMed
description It is technically difficult to acquire large-field images under the complexity and cost restrictions of a diagnostic and instant field research purpose. The goal of the introduced large-field imaging system is to achieve a tolerable resolution for detecting microscale particles or objects in the entire image field without the field-curvature effect, while maintaining a cost-effective procedure and simple design. To use a single commercial lens for imaging a large field, the design attempts to fabricate a curved microfluidic chamber. This imaging technique improves the field curvature and distortion at an acceptable level of particle detection. This study examines Paramecium caudatum microswimmers to track their motion dynamics in different viscous media with imaging techniques. In addition, the study found that the average speed for P. caudatum was 60 µm/s, with a standard deviation of ±12 µm/s from microscopic imaging of the original medium of the sample, which leads to a variation of 20% from the average measurement. In contrast, from large-field imaging, the average speeds of P. caudatum were 63 µm/s and 68 µm/s in the flat and curved chambers, respectively, with the same medium viscosity. Furthermore, the standard deviations that were observed were ±7 µm/s and ±4 µm/s and the variations from the average speed were calculated as 11% and 5.8% for the flat and curved chambers, respectively. The proposed methodology can be applied to measure the locomotion of the microswimmer at small scales with high precision.
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spelling pubmed-61874822018-11-01 Long-Term Tracking of Free-Swimming Paramecium caudatum in Viscous Media Using a Curved Sample Chamber Shourav, Mohiuddin Khan Kim, Jung Kyung Micromachines (Basel) Article It is technically difficult to acquire large-field images under the complexity and cost restrictions of a diagnostic and instant field research purpose. The goal of the introduced large-field imaging system is to achieve a tolerable resolution for detecting microscale particles or objects in the entire image field without the field-curvature effect, while maintaining a cost-effective procedure and simple design. To use a single commercial lens for imaging a large field, the design attempts to fabricate a curved microfluidic chamber. This imaging technique improves the field curvature and distortion at an acceptable level of particle detection. This study examines Paramecium caudatum microswimmers to track their motion dynamics in different viscous media with imaging techniques. In addition, the study found that the average speed for P. caudatum was 60 µm/s, with a standard deviation of ±12 µm/s from microscopic imaging of the original medium of the sample, which leads to a variation of 20% from the average measurement. In contrast, from large-field imaging, the average speeds of P. caudatum were 63 µm/s and 68 µm/s in the flat and curved chambers, respectively, with the same medium viscosity. Furthermore, the standard deviations that were observed were ±7 µm/s and ±4 µm/s and the variations from the average speed were calculated as 11% and 5.8% for the flat and curved chambers, respectively. The proposed methodology can be applied to measure the locomotion of the microswimmer at small scales with high precision. MDPI 2017-12-28 /pmc/articles/PMC6187482/ /pubmed/30393284 http://dx.doi.org/10.3390/mi9010007 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shourav, Mohiuddin Khan
Kim, Jung Kyung
Long-Term Tracking of Free-Swimming Paramecium caudatum in Viscous Media Using a Curved Sample Chamber
title Long-Term Tracking of Free-Swimming Paramecium caudatum in Viscous Media Using a Curved Sample Chamber
title_full Long-Term Tracking of Free-Swimming Paramecium caudatum in Viscous Media Using a Curved Sample Chamber
title_fullStr Long-Term Tracking of Free-Swimming Paramecium caudatum in Viscous Media Using a Curved Sample Chamber
title_full_unstemmed Long-Term Tracking of Free-Swimming Paramecium caudatum in Viscous Media Using a Curved Sample Chamber
title_short Long-Term Tracking of Free-Swimming Paramecium caudatum in Viscous Media Using a Curved Sample Chamber
title_sort long-term tracking of free-swimming paramecium caudatum in viscous media using a curved sample chamber
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187482/
https://www.ncbi.nlm.nih.gov/pubmed/30393284
http://dx.doi.org/10.3390/mi9010007
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