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Fabrication of 3D Capillary Vessel Models with Circulatory Connection Ports

Bionic microscopic vessel models can contribute to the development of vascular treatment skills and techniques for clinical training. Most microscopic vessel models are limited to two dimensions, but three-dimensional (3D) models are important for surgery, such as on retina microscopic vessels, for...

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Autores principales: Gallab, Mahmoud, Tomita, Kyohei, Omata, Seiji, Arai, Fumihito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187555/
https://www.ncbi.nlm.nih.gov/pubmed/30424035
http://dx.doi.org/10.3390/mi9030101
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author Gallab, Mahmoud
Tomita, Kyohei
Omata, Seiji
Arai, Fumihito
author_facet Gallab, Mahmoud
Tomita, Kyohei
Omata, Seiji
Arai, Fumihito
author_sort Gallab, Mahmoud
collection PubMed
description Bionic microscopic vessel models can contribute to the development of vascular treatment skills and techniques for clinical training. Most microscopic vessel models are limited to two dimensions, but three-dimensional (3D) models are important for surgery, such as on retina microscopic vessels, for the observation of colon microvessels, for measuring the deformability of red blood cell (RBC), and so on. Therefore, bionic 3D blood vessel models are increasingly in demand. For this reason, it is necessary to establish 3D fabrication techniques for microchannels. In this study, we established two fabrication methods for 3D microfluidic devices for the development of microscopic vessel models. First, we employed an exposure method using photolithographic technology. Second, we employed a 3D method using femtosecond laser and mask hybrid exposure (FMEx). Both methods made it possible to fabricate a millimeter-scale 3D structure with a submicrometer resolution and achieve an easy injection of solution. This is because it was possible to fabricate typical microfluidic channels used for model inlet and outlet ports. Furthermore, in the FMEx method, we employed an acid-diffusion effect using a chemically amplified resist to form a circular channel cross-section. The acid-diffusion effect made it realizable to fabricate a smooth surface independent of the laser scanning line width. Thus, we succeeded in establishing two methods for the fabrication of bionic 3D microfluidic devices with microfluidic channels having diameters of 15–16 µm for mimicking capillary vessels.
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spelling pubmed-61875552018-11-01 Fabrication of 3D Capillary Vessel Models with Circulatory Connection Ports Gallab, Mahmoud Tomita, Kyohei Omata, Seiji Arai, Fumihito Micromachines (Basel) Article Bionic microscopic vessel models can contribute to the development of vascular treatment skills and techniques for clinical training. Most microscopic vessel models are limited to two dimensions, but three-dimensional (3D) models are important for surgery, such as on retina microscopic vessels, for the observation of colon microvessels, for measuring the deformability of red blood cell (RBC), and so on. Therefore, bionic 3D blood vessel models are increasingly in demand. For this reason, it is necessary to establish 3D fabrication techniques for microchannels. In this study, we established two fabrication methods for 3D microfluidic devices for the development of microscopic vessel models. First, we employed an exposure method using photolithographic technology. Second, we employed a 3D method using femtosecond laser and mask hybrid exposure (FMEx). Both methods made it possible to fabricate a millimeter-scale 3D structure with a submicrometer resolution and achieve an easy injection of solution. This is because it was possible to fabricate typical microfluidic channels used for model inlet and outlet ports. Furthermore, in the FMEx method, we employed an acid-diffusion effect using a chemically amplified resist to form a circular channel cross-section. The acid-diffusion effect made it realizable to fabricate a smooth surface independent of the laser scanning line width. Thus, we succeeded in establishing two methods for the fabrication of bionic 3D microfluidic devices with microfluidic channels having diameters of 15–16 µm for mimicking capillary vessels. MDPI 2018-02-28 /pmc/articles/PMC6187555/ /pubmed/30424035 http://dx.doi.org/10.3390/mi9030101 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gallab, Mahmoud
Tomita, Kyohei
Omata, Seiji
Arai, Fumihito
Fabrication of 3D Capillary Vessel Models with Circulatory Connection Ports
title Fabrication of 3D Capillary Vessel Models with Circulatory Connection Ports
title_full Fabrication of 3D Capillary Vessel Models with Circulatory Connection Ports
title_fullStr Fabrication of 3D Capillary Vessel Models with Circulatory Connection Ports
title_full_unstemmed Fabrication of 3D Capillary Vessel Models with Circulatory Connection Ports
title_short Fabrication of 3D Capillary Vessel Models with Circulatory Connection Ports
title_sort fabrication of 3d capillary vessel models with circulatory connection ports
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187555/
https://www.ncbi.nlm.nih.gov/pubmed/30424035
http://dx.doi.org/10.3390/mi9030101
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