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Nano and Microsensors for Mammalian Cell Studies
This review presents several sensors with dimensions at the nano- and micro-scale used for biological applications. Two types of cantilever beams employed as highly sensitive temperature sensors with biological applications will be presented. One type of cantilever beam is fabricated from composite...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187600/ https://www.ncbi.nlm.nih.gov/pubmed/30424372 http://dx.doi.org/10.3390/mi9090439 |
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author | Voiculescu, Ioana Toda, Masaya Inomata, Naoki Ono, Takahito Li, Fang |
author_facet | Voiculescu, Ioana Toda, Masaya Inomata, Naoki Ono, Takahito Li, Fang |
author_sort | Voiculescu, Ioana |
collection | PubMed |
description | This review presents several sensors with dimensions at the nano- and micro-scale used for biological applications. Two types of cantilever beams employed as highly sensitive temperature sensors with biological applications will be presented. One type of cantilever beam is fabricated from composite materials and is operated in the deflection mode. In order to achieve the high sensitivity required for detection of heat generated by a single mammalian cell, the cantilever beam temperature sensor presented in this review was microprocessed with a length at the microscale and a thickness in the nanoscale dimension. The second type of cantilever beam presented in this review was operated in the resonant frequency regime. The working principle of the vibrating cantilever beam temperature sensor is based on shifts in resonant frequency in response to temperature variations generated by mammalian cells. Besides the cantilever beam biosensors, two biosensors based on the electric cell-substrate impedance sensing (ECIS) used to monitor mammalian cells attachment and viability will be presented in this review. These ECIS sensors have dimensions at the microscale, with the gold films used for electrodes having thickness at the nanoscale. These micro/nano biosensors and their mammalian cell applications presented in the review demonstrates the diversity of the biosensor technology and applications. |
format | Online Article Text |
id | pubmed-6187600 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61876002018-11-01 Nano and Microsensors for Mammalian Cell Studies Voiculescu, Ioana Toda, Masaya Inomata, Naoki Ono, Takahito Li, Fang Micromachines (Basel) Review This review presents several sensors with dimensions at the nano- and micro-scale used for biological applications. Two types of cantilever beams employed as highly sensitive temperature sensors with biological applications will be presented. One type of cantilever beam is fabricated from composite materials and is operated in the deflection mode. In order to achieve the high sensitivity required for detection of heat generated by a single mammalian cell, the cantilever beam temperature sensor presented in this review was microprocessed with a length at the microscale and a thickness in the nanoscale dimension. The second type of cantilever beam presented in this review was operated in the resonant frequency regime. The working principle of the vibrating cantilever beam temperature sensor is based on shifts in resonant frequency in response to temperature variations generated by mammalian cells. Besides the cantilever beam biosensors, two biosensors based on the electric cell-substrate impedance sensing (ECIS) used to monitor mammalian cells attachment and viability will be presented in this review. These ECIS sensors have dimensions at the microscale, with the gold films used for electrodes having thickness at the nanoscale. These micro/nano biosensors and their mammalian cell applications presented in the review demonstrates the diversity of the biosensor technology and applications. MDPI 2018-08-31 /pmc/articles/PMC6187600/ /pubmed/30424372 http://dx.doi.org/10.3390/mi9090439 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Voiculescu, Ioana Toda, Masaya Inomata, Naoki Ono, Takahito Li, Fang Nano and Microsensors for Mammalian Cell Studies |
title | Nano and Microsensors for Mammalian Cell Studies |
title_full | Nano and Microsensors for Mammalian Cell Studies |
title_fullStr | Nano and Microsensors for Mammalian Cell Studies |
title_full_unstemmed | Nano and Microsensors for Mammalian Cell Studies |
title_short | Nano and Microsensors for Mammalian Cell Studies |
title_sort | nano and microsensors for mammalian cell studies |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187600/ https://www.ncbi.nlm.nih.gov/pubmed/30424372 http://dx.doi.org/10.3390/mi9090439 |
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