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Assessment of the Deformability and Velocity of Healthy and Artificially Impaired Red Blood Cells in Narrow Polydimethylsiloxane (PDMS) Microchannels

Malaria is one of the leading causes of death in underdeveloped regions. Thus, the development of rapid, efficient, and competitive diagnostic techniques is essential. This work reports a study of the deformability and velocity assessment of healthy and artificially impaired red blood cells (RBCs),...

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Detalles Bibliográficos
Autores principales: Boas, Liliana Vilas, Faustino, Vera, Lima, Rui, Miranda, João Mário, Minas, Graça, Fernandes, Carla Sofia Veiga, Catarino, Susana Oliveira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187685/
https://www.ncbi.nlm.nih.gov/pubmed/30424317
http://dx.doi.org/10.3390/mi9080384
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author Boas, Liliana Vilas
Faustino, Vera
Lima, Rui
Miranda, João Mário
Minas, Graça
Fernandes, Carla Sofia Veiga
Catarino, Susana Oliveira
author_facet Boas, Liliana Vilas
Faustino, Vera
Lima, Rui
Miranda, João Mário
Minas, Graça
Fernandes, Carla Sofia Veiga
Catarino, Susana Oliveira
author_sort Boas, Liliana Vilas
collection PubMed
description Malaria is one of the leading causes of death in underdeveloped regions. Thus, the development of rapid, efficient, and competitive diagnostic techniques is essential. This work reports a study of the deformability and velocity assessment of healthy and artificially impaired red blood cells (RBCs), with the purpose of potentially mimicking malaria effects, in narrow polydimethylsiloxane microchannels. To obtain impaired RBCs, their properties were modified by adding, to the RBCs, different concentrations of glucose, glutaraldehyde, or diamide, in order to increase the cells’ rigidity. The effects of the RBCs’ artificial stiffening were evaluated by combining image analysis techniques with microchannels with a contraction width of 8 µm, making it possible to measure the cells’ deformability and velocity of both healthy and modified RBCs. The results showed that healthy RBCs naturally deform when they cross the contractions and rapidly recover their original shape. In contrast, for the modified samples with high concentration of chemicals, the same did not occur. Additionally, for all the tested modification methods, the results have shown a decrease in the RBCs’ deformability and velocity as the cells’ rigidity increases, when compared to the behavior of healthy RBCs samples. These results show the ability of the image analysis tools combined with microchannel contractions to obtain crucial information on the pathological blood phenomena in microcirculation. Particularly, it was possible to measure the deformability of the RBCs and their velocity, resulting in a velocity/deformability relation in the microchannel. This correlation shows great potential to relate the RBCs’ behavior with the various stages of malaria, helping to establish the development of new diagnostic systems towards point-of-care devices.
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spelling pubmed-61876852018-11-01 Assessment of the Deformability and Velocity of Healthy and Artificially Impaired Red Blood Cells in Narrow Polydimethylsiloxane (PDMS) Microchannels Boas, Liliana Vilas Faustino, Vera Lima, Rui Miranda, João Mário Minas, Graça Fernandes, Carla Sofia Veiga Catarino, Susana Oliveira Micromachines (Basel) Article Malaria is one of the leading causes of death in underdeveloped regions. Thus, the development of rapid, efficient, and competitive diagnostic techniques is essential. This work reports a study of the deformability and velocity assessment of healthy and artificially impaired red blood cells (RBCs), with the purpose of potentially mimicking malaria effects, in narrow polydimethylsiloxane microchannels. To obtain impaired RBCs, their properties were modified by adding, to the RBCs, different concentrations of glucose, glutaraldehyde, or diamide, in order to increase the cells’ rigidity. The effects of the RBCs’ artificial stiffening were evaluated by combining image analysis techniques with microchannels with a contraction width of 8 µm, making it possible to measure the cells’ deformability and velocity of both healthy and modified RBCs. The results showed that healthy RBCs naturally deform when they cross the contractions and rapidly recover their original shape. In contrast, for the modified samples with high concentration of chemicals, the same did not occur. Additionally, for all the tested modification methods, the results have shown a decrease in the RBCs’ deformability and velocity as the cells’ rigidity increases, when compared to the behavior of healthy RBCs samples. These results show the ability of the image analysis tools combined with microchannel contractions to obtain crucial information on the pathological blood phenomena in microcirculation. Particularly, it was possible to measure the deformability of the RBCs and their velocity, resulting in a velocity/deformability relation in the microchannel. This correlation shows great potential to relate the RBCs’ behavior with the various stages of malaria, helping to establish the development of new diagnostic systems towards point-of-care devices. MDPI 2018-08-02 /pmc/articles/PMC6187685/ /pubmed/30424317 http://dx.doi.org/10.3390/mi9080384 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Boas, Liliana Vilas
Faustino, Vera
Lima, Rui
Miranda, João Mário
Minas, Graça
Fernandes, Carla Sofia Veiga
Catarino, Susana Oliveira
Assessment of the Deformability and Velocity of Healthy and Artificially Impaired Red Blood Cells in Narrow Polydimethylsiloxane (PDMS) Microchannels
title Assessment of the Deformability and Velocity of Healthy and Artificially Impaired Red Blood Cells in Narrow Polydimethylsiloxane (PDMS) Microchannels
title_full Assessment of the Deformability and Velocity of Healthy and Artificially Impaired Red Blood Cells in Narrow Polydimethylsiloxane (PDMS) Microchannels
title_fullStr Assessment of the Deformability and Velocity of Healthy and Artificially Impaired Red Blood Cells in Narrow Polydimethylsiloxane (PDMS) Microchannels
title_full_unstemmed Assessment of the Deformability and Velocity of Healthy and Artificially Impaired Red Blood Cells in Narrow Polydimethylsiloxane (PDMS) Microchannels
title_short Assessment of the Deformability and Velocity of Healthy and Artificially Impaired Red Blood Cells in Narrow Polydimethylsiloxane (PDMS) Microchannels
title_sort assessment of the deformability and velocity of healthy and artificially impaired red blood cells in narrow polydimethylsiloxane (pdms) microchannels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187685/
https://www.ncbi.nlm.nih.gov/pubmed/30424317
http://dx.doi.org/10.3390/mi9080384
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