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Fluorescent Nanodiamond Applications for Cellular Process Sensing and Cell Tracking

Diamond nanocrystals smaller than 100 nm (nanodiamonds) are now recognized to be highly biocompatible. They can be made fluorescent with perfect photostability by creating nitrogen-vacancy (NV) color centers in the diamond lattice. The resulting fluorescent nanodiamonds (FND) have been used since th...

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Autores principales: Claveau, Sandra, Bertrand, Jean-Rémi, Treussart, François
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187705/
https://www.ncbi.nlm.nih.gov/pubmed/30424180
http://dx.doi.org/10.3390/mi9050247
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author Claveau, Sandra
Bertrand, Jean-Rémi
Treussart, François
author_facet Claveau, Sandra
Bertrand, Jean-Rémi
Treussart, François
author_sort Claveau, Sandra
collection PubMed
description Diamond nanocrystals smaller than 100 nm (nanodiamonds) are now recognized to be highly biocompatible. They can be made fluorescent with perfect photostability by creating nitrogen-vacancy (NV) color centers in the diamond lattice. The resulting fluorescent nanodiamonds (FND) have been used since the late 2000s as fluorescent probes for short- or long-term analysis. FND can be used both at the subcellular scale and the single cell scale. Their limited sub-diffraction size allows them to track intracellular processes with high spatio-temporal resolution and high contrast from the surrounding environment. FND can also track the fate of therapeutic compounds or whole cells in the organs of an organism. This review presents examples of FND applications (1) for intra and intercellular molecular processes sensing, also introducing the different potential biosensing applications based on the optically detectable electron spin resonance of NV(−) centers; and (2) for tracking, firstly, FND themselves to determine their biodistribution, and secondly, using FND as cell tracking probes for diagnosis or follow-up purposes in oncology and regenerative medicine.
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spelling pubmed-61877052018-11-01 Fluorescent Nanodiamond Applications for Cellular Process Sensing and Cell Tracking Claveau, Sandra Bertrand, Jean-Rémi Treussart, François Micromachines (Basel) Review Diamond nanocrystals smaller than 100 nm (nanodiamonds) are now recognized to be highly biocompatible. They can be made fluorescent with perfect photostability by creating nitrogen-vacancy (NV) color centers in the diamond lattice. The resulting fluorescent nanodiamonds (FND) have been used since the late 2000s as fluorescent probes for short- or long-term analysis. FND can be used both at the subcellular scale and the single cell scale. Their limited sub-diffraction size allows them to track intracellular processes with high spatio-temporal resolution and high contrast from the surrounding environment. FND can also track the fate of therapeutic compounds or whole cells in the organs of an organism. This review presents examples of FND applications (1) for intra and intercellular molecular processes sensing, also introducing the different potential biosensing applications based on the optically detectable electron spin resonance of NV(−) centers; and (2) for tracking, firstly, FND themselves to determine their biodistribution, and secondly, using FND as cell tracking probes for diagnosis or follow-up purposes in oncology and regenerative medicine. MDPI 2018-05-18 /pmc/articles/PMC6187705/ /pubmed/30424180 http://dx.doi.org/10.3390/mi9050247 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Claveau, Sandra
Bertrand, Jean-Rémi
Treussart, François
Fluorescent Nanodiamond Applications for Cellular Process Sensing and Cell Tracking
title Fluorescent Nanodiamond Applications for Cellular Process Sensing and Cell Tracking
title_full Fluorescent Nanodiamond Applications for Cellular Process Sensing and Cell Tracking
title_fullStr Fluorescent Nanodiamond Applications for Cellular Process Sensing and Cell Tracking
title_full_unstemmed Fluorescent Nanodiamond Applications for Cellular Process Sensing and Cell Tracking
title_short Fluorescent Nanodiamond Applications for Cellular Process Sensing and Cell Tracking
title_sort fluorescent nanodiamond applications for cellular process sensing and cell tracking
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187705/
https://www.ncbi.nlm.nih.gov/pubmed/30424180
http://dx.doi.org/10.3390/mi9050247
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