Cargando…
Circulating angiogenic stem cells in type 2 diabetes are associated with glycemic control and endothelial dysfunction
Circulating angiogenic cells (CACs) of various described phenotypes participate in the regeneration of the damaged endothelium, but the abundance of these cells is highly influenced by external cues including diabetes. It is not entirely clear which CAC populations are most reflective of endothelial...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6188890/ https://www.ncbi.nlm.nih.gov/pubmed/30321232 http://dx.doi.org/10.1371/journal.pone.0205851 |
_version_ | 1783363256338874368 |
---|---|
author | Zafar, Nagma Krishnasamy, Sathya S. Shah, Jasmit Rai, Shesh N. Riggs, Daniel W. Bhatnagar, Aruni O’Toole, Timothy E. |
author_facet | Zafar, Nagma Krishnasamy, Sathya S. Shah, Jasmit Rai, Shesh N. Riggs, Daniel W. Bhatnagar, Aruni O’Toole, Timothy E. |
author_sort | Zafar, Nagma |
collection | PubMed |
description | Circulating angiogenic cells (CACs) of various described phenotypes participate in the regeneration of the damaged endothelium, but the abundance of these cells is highly influenced by external cues including diabetes. It is not entirely clear which CAC populations are most reflective of endothelial function nor which are impacted by diabetes. To answer these questions, we enrolled a human cohort with variable CVD risk and determined relationships between stratified levels of CACs and indices of diabetes and vascular function. We also determined associations between CAC functional markers and diabetes and identified pro-angiogenic molecules which are impacted by diabetes. We found that subjects with low levels of CD34(+)/AC133(+)/CD31(+)/CD45(dim) cells (CAC-3) had a significantly higher incidence of diabetes (p = 0.004), higher HbA1c levels (p = 0.049) and higher CVD risk scores. Furthermore, there was an association between low CAC-3 levels and impaired vascular function (p = 0.023). These cells from diabetics had reduced levels of CXCR4 and VEGFR2, while diabetics had higher levels of certain cytokines and pro-angiogenic molecules. These results suggest that quantitative and functional defects of CD34(+)/AC133(+)/CD31(+)/CD45(dim) cells are associated with diabetes and vascular impairment and that this cell type may be a prognostic indicator of CVD and vascular dysfunction. |
format | Online Article Text |
id | pubmed-6188890 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-61888902018-10-25 Circulating angiogenic stem cells in type 2 diabetes are associated with glycemic control and endothelial dysfunction Zafar, Nagma Krishnasamy, Sathya S. Shah, Jasmit Rai, Shesh N. Riggs, Daniel W. Bhatnagar, Aruni O’Toole, Timothy E. PLoS One Research Article Circulating angiogenic cells (CACs) of various described phenotypes participate in the regeneration of the damaged endothelium, but the abundance of these cells is highly influenced by external cues including diabetes. It is not entirely clear which CAC populations are most reflective of endothelial function nor which are impacted by diabetes. To answer these questions, we enrolled a human cohort with variable CVD risk and determined relationships between stratified levels of CACs and indices of diabetes and vascular function. We also determined associations between CAC functional markers and diabetes and identified pro-angiogenic molecules which are impacted by diabetes. We found that subjects with low levels of CD34(+)/AC133(+)/CD31(+)/CD45(dim) cells (CAC-3) had a significantly higher incidence of diabetes (p = 0.004), higher HbA1c levels (p = 0.049) and higher CVD risk scores. Furthermore, there was an association between low CAC-3 levels and impaired vascular function (p = 0.023). These cells from diabetics had reduced levels of CXCR4 and VEGFR2, while diabetics had higher levels of certain cytokines and pro-angiogenic molecules. These results suggest that quantitative and functional defects of CD34(+)/AC133(+)/CD31(+)/CD45(dim) cells are associated with diabetes and vascular impairment and that this cell type may be a prognostic indicator of CVD and vascular dysfunction. Public Library of Science 2018-10-15 /pmc/articles/PMC6188890/ /pubmed/30321232 http://dx.doi.org/10.1371/journal.pone.0205851 Text en © 2018 Zafar et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zafar, Nagma Krishnasamy, Sathya S. Shah, Jasmit Rai, Shesh N. Riggs, Daniel W. Bhatnagar, Aruni O’Toole, Timothy E. Circulating angiogenic stem cells in type 2 diabetes are associated with glycemic control and endothelial dysfunction |
title | Circulating angiogenic stem cells in type 2 diabetes are associated with glycemic control and endothelial dysfunction |
title_full | Circulating angiogenic stem cells in type 2 diabetes are associated with glycemic control and endothelial dysfunction |
title_fullStr | Circulating angiogenic stem cells in type 2 diabetes are associated with glycemic control and endothelial dysfunction |
title_full_unstemmed | Circulating angiogenic stem cells in type 2 diabetes are associated with glycemic control and endothelial dysfunction |
title_short | Circulating angiogenic stem cells in type 2 diabetes are associated with glycemic control and endothelial dysfunction |
title_sort | circulating angiogenic stem cells in type 2 diabetes are associated with glycemic control and endothelial dysfunction |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6188890/ https://www.ncbi.nlm.nih.gov/pubmed/30321232 http://dx.doi.org/10.1371/journal.pone.0205851 |
work_keys_str_mv | AT zafarnagma circulatingangiogenicstemcellsintype2diabetesareassociatedwithglycemiccontrolandendothelialdysfunction AT krishnasamysathyas circulatingangiogenicstemcellsintype2diabetesareassociatedwithglycemiccontrolandendothelialdysfunction AT shahjasmit circulatingangiogenicstemcellsintype2diabetesareassociatedwithglycemiccontrolandendothelialdysfunction AT raisheshn circulatingangiogenicstemcellsintype2diabetesareassociatedwithglycemiccontrolandendothelialdysfunction AT riggsdanielw circulatingangiogenicstemcellsintype2diabetesareassociatedwithglycemiccontrolandendothelialdysfunction AT bhatnagararuni circulatingangiogenicstemcellsintype2diabetesareassociatedwithglycemiccontrolandendothelialdysfunction AT otooletimothye circulatingangiogenicstemcellsintype2diabetesareassociatedwithglycemiccontrolandendothelialdysfunction |