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HIV-1 Tat interactions with cellular 7SK and viral TAR RNAs identifies dual structural mimicry
The HIV Tat protein competes with the 7SK:HEXIM interaction to hijack pTEFb from 7SK snRNP and recruit it to the TAR motif on stalled viral transcripts. Here we solve structures of 7SK stemloop-1 and TAR in complex with Tat’s RNA binding domain (RBD) to gain insights into this process. We find that...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189040/ https://www.ncbi.nlm.nih.gov/pubmed/30323330 http://dx.doi.org/10.1038/s41467-018-06591-6 |
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author | Pham, Vincent V. Salguero, Carolina Khan, Shamsun Nahar Meagher, Jennifer L. Brown, W. Clay Humbert, Nicolas de Rocquigny, Hugues Smith, Janet L. D’Souza, Victoria M. |
author_facet | Pham, Vincent V. Salguero, Carolina Khan, Shamsun Nahar Meagher, Jennifer L. Brown, W. Clay Humbert, Nicolas de Rocquigny, Hugues Smith, Janet L. D’Souza, Victoria M. |
author_sort | Pham, Vincent V. |
collection | PubMed |
description | The HIV Tat protein competes with the 7SK:HEXIM interaction to hijack pTEFb from 7SK snRNP and recruit it to the TAR motif on stalled viral transcripts. Here we solve structures of 7SK stemloop-1 and TAR in complex with Tat’s RNA binding domain (RBD) to gain insights into this process. We find that 7SK is peppered with arginine sandwich motifs (ASM)—three classical and one with a pseudo configuration. Despite having similar RBDs, the presence of an additional arginine, R52, confers Tat the ability to remodel the pseudo configuration, required for HEXIM binding, into a classical sandwich, thus displacing HEXIM. Tat also uses R52 to remodel the TAR bulge into an ASM whose structure is identical to that of the remodeled ASM in 7SK. Together, our structures reveal a dual structural mimicry wherein viral Tat and TAR have co-opted structural motifs present in cellular HEXIM and 7SK for productive transcription of its genome. |
format | Online Article Text |
id | pubmed-6189040 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-61890402018-10-17 HIV-1 Tat interactions with cellular 7SK and viral TAR RNAs identifies dual structural mimicry Pham, Vincent V. Salguero, Carolina Khan, Shamsun Nahar Meagher, Jennifer L. Brown, W. Clay Humbert, Nicolas de Rocquigny, Hugues Smith, Janet L. D’Souza, Victoria M. Nat Commun Article The HIV Tat protein competes with the 7SK:HEXIM interaction to hijack pTEFb from 7SK snRNP and recruit it to the TAR motif on stalled viral transcripts. Here we solve structures of 7SK stemloop-1 and TAR in complex with Tat’s RNA binding domain (RBD) to gain insights into this process. We find that 7SK is peppered with arginine sandwich motifs (ASM)—three classical and one with a pseudo configuration. Despite having similar RBDs, the presence of an additional arginine, R52, confers Tat the ability to remodel the pseudo configuration, required for HEXIM binding, into a classical sandwich, thus displacing HEXIM. Tat also uses R52 to remodel the TAR bulge into an ASM whose structure is identical to that of the remodeled ASM in 7SK. Together, our structures reveal a dual structural mimicry wherein viral Tat and TAR have co-opted structural motifs present in cellular HEXIM and 7SK for productive transcription of its genome. Nature Publishing Group UK 2018-10-15 /pmc/articles/PMC6189040/ /pubmed/30323330 http://dx.doi.org/10.1038/s41467-018-06591-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Pham, Vincent V. Salguero, Carolina Khan, Shamsun Nahar Meagher, Jennifer L. Brown, W. Clay Humbert, Nicolas de Rocquigny, Hugues Smith, Janet L. D’Souza, Victoria M. HIV-1 Tat interactions with cellular 7SK and viral TAR RNAs identifies dual structural mimicry |
title | HIV-1 Tat interactions with cellular 7SK and viral TAR RNAs identifies dual structural mimicry |
title_full | HIV-1 Tat interactions with cellular 7SK and viral TAR RNAs identifies dual structural mimicry |
title_fullStr | HIV-1 Tat interactions with cellular 7SK and viral TAR RNAs identifies dual structural mimicry |
title_full_unstemmed | HIV-1 Tat interactions with cellular 7SK and viral TAR RNAs identifies dual structural mimicry |
title_short | HIV-1 Tat interactions with cellular 7SK and viral TAR RNAs identifies dual structural mimicry |
title_sort | hiv-1 tat interactions with cellular 7sk and viral tar rnas identifies dual structural mimicry |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189040/ https://www.ncbi.nlm.nih.gov/pubmed/30323330 http://dx.doi.org/10.1038/s41467-018-06591-6 |
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