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Tridimensional infiltration of DNA viruses into the host genome shows preferential contact with active chromatin

Whether non-integrated viral DNAs distribute randomly or target specific positions within the higher-order architecture of mammalian genomes remains largely unknown. Here we use Hi-C and viral DNA capture (CHi-C) in primary human hepatocytes infected by either hepatitis B virus (HBV) or adenovirus t...

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Autores principales: Moreau, Pierrick, Cournac, Axel, Palumbo, Gianna Aurora, Marbouty, Martial, Mortaza, Shogofa, Thierry, Agnes, Cairo, Stefano, Lavigne, Marc, Koszul, Romain, Neuveut, Christine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189100/
https://www.ncbi.nlm.nih.gov/pubmed/30323189
http://dx.doi.org/10.1038/s41467-018-06739-4
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author Moreau, Pierrick
Cournac, Axel
Palumbo, Gianna Aurora
Marbouty, Martial
Mortaza, Shogofa
Thierry, Agnes
Cairo, Stefano
Lavigne, Marc
Koszul, Romain
Neuveut, Christine
author_facet Moreau, Pierrick
Cournac, Axel
Palumbo, Gianna Aurora
Marbouty, Martial
Mortaza, Shogofa
Thierry, Agnes
Cairo, Stefano
Lavigne, Marc
Koszul, Romain
Neuveut, Christine
author_sort Moreau, Pierrick
collection PubMed
description Whether non-integrated viral DNAs distribute randomly or target specific positions within the higher-order architecture of mammalian genomes remains largely unknown. Here we use Hi-C and viral DNA capture (CHi-C) in primary human hepatocytes infected by either hepatitis B virus (HBV) or adenovirus type 5 (Ad5) virus to show that they adopt different strategies in their respective positioning at active chromatin. HBV contacts preferentially CpG islands (CGIs) enriched in Cfp1 a factor required for its transcription. These CGIs are often associated with highly expressed genes (HEG) and genes deregulated during infection. Ad5 DNA interacts preferentially with transcription start sites (TSSs) and enhancers of HEG, as well as genes upregulated during infection. These results show that DNA viruses use different strategies to infiltrate genomic 3D networks and target specific regions. This targeting may facilitate the recruitment of transcription factors necessary for their own replication and contribute to the deregulation of cellular gene expression.
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spelling pubmed-61891002018-10-17 Tridimensional infiltration of DNA viruses into the host genome shows preferential contact with active chromatin Moreau, Pierrick Cournac, Axel Palumbo, Gianna Aurora Marbouty, Martial Mortaza, Shogofa Thierry, Agnes Cairo, Stefano Lavigne, Marc Koszul, Romain Neuveut, Christine Nat Commun Article Whether non-integrated viral DNAs distribute randomly or target specific positions within the higher-order architecture of mammalian genomes remains largely unknown. Here we use Hi-C and viral DNA capture (CHi-C) in primary human hepatocytes infected by either hepatitis B virus (HBV) or adenovirus type 5 (Ad5) virus to show that they adopt different strategies in their respective positioning at active chromatin. HBV contacts preferentially CpG islands (CGIs) enriched in Cfp1 a factor required for its transcription. These CGIs are often associated with highly expressed genes (HEG) and genes deregulated during infection. Ad5 DNA interacts preferentially with transcription start sites (TSSs) and enhancers of HEG, as well as genes upregulated during infection. These results show that DNA viruses use different strategies to infiltrate genomic 3D networks and target specific regions. This targeting may facilitate the recruitment of transcription factors necessary for their own replication and contribute to the deregulation of cellular gene expression. Nature Publishing Group UK 2018-10-15 /pmc/articles/PMC6189100/ /pubmed/30323189 http://dx.doi.org/10.1038/s41467-018-06739-4 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Moreau, Pierrick
Cournac, Axel
Palumbo, Gianna Aurora
Marbouty, Martial
Mortaza, Shogofa
Thierry, Agnes
Cairo, Stefano
Lavigne, Marc
Koszul, Romain
Neuveut, Christine
Tridimensional infiltration of DNA viruses into the host genome shows preferential contact with active chromatin
title Tridimensional infiltration of DNA viruses into the host genome shows preferential contact with active chromatin
title_full Tridimensional infiltration of DNA viruses into the host genome shows preferential contact with active chromatin
title_fullStr Tridimensional infiltration of DNA viruses into the host genome shows preferential contact with active chromatin
title_full_unstemmed Tridimensional infiltration of DNA viruses into the host genome shows preferential contact with active chromatin
title_short Tridimensional infiltration of DNA viruses into the host genome shows preferential contact with active chromatin
title_sort tridimensional infiltration of dna viruses into the host genome shows preferential contact with active chromatin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189100/
https://www.ncbi.nlm.nih.gov/pubmed/30323189
http://dx.doi.org/10.1038/s41467-018-06739-4
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