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Reconsideration of the evaluation criteria for bull ejaculated sperm motility in the context of rotation

Progressive movement of spermatozoa has conventionally been regarded as a good indicator of motility. However, bull spermatozoa exhibit two types of progressive movement: progressive/planar movement without rotation and progressive/helical movement with rotation. The aim of this study was to reconsi...

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Autores principales: YAMADA, Ayano, SAKASE, Mitsuhiro, FUKUSHIMA, Moriyuki, HARAYAMA, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189569/
https://www.ncbi.nlm.nih.gov/pubmed/29952339
http://dx.doi.org/10.1262/jrd.2018-036
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author YAMADA, Ayano
SAKASE, Mitsuhiro
FUKUSHIMA, Moriyuki
HARAYAMA, Hiroshi
author_facet YAMADA, Ayano
SAKASE, Mitsuhiro
FUKUSHIMA, Moriyuki
HARAYAMA, Hiroshi
author_sort YAMADA, Ayano
collection PubMed
description Progressive movement of spermatozoa has conventionally been regarded as a good indicator of motility. However, bull spermatozoa exhibit two types of progressive movement: progressive/planar movement without rotation and progressive/helical movement with rotation. The aim of this study was to reconsider the evaluation criteria of bull ejaculated sperm motility in the context of rotation. Here, we compared the movement patterns of ejaculated spermatozoa with relatively high and low protein kinase A (PKA)-mediated signaling activities, because sperm motility is positively regulated by PKA-mediated signaling activities. We prepared sperm samples with high and low PKA-mediated signaling activities by suspending spermatozoa in media containing either the stimulator (NaHCO(3)) or inhibitor (KH-7) of adenylyl cyclase 10, and we then investigated movement patterns and relative velocities using a microscopic high-speed camera and recording system. In the control medium without NaHCO(3) and KH-7, most spermatozoa exhibited round/planar movement without rotation and asymmetrical bends in the principal pieces. NaHCO(3) significantly promoted changes in movement patterns from round/planar movement to progressive/planar movement (without rotation) as well as symmetrization of flagellar bends and increased relative velocities. KH-7 significantly increased spermatozoa exhibiting progressive/helical movement (with rotation), decreased relative velocities, and symmetrized flagellar bends with a reduction in their size. These indicate that progressive/planar movement (without rotation) and fast movement characterize the movement patterns of bull ejaculated spermatozoa with high PKA-mediated signaling activities. A sign of reduced PKA-mediated signaling activity is not only slow movement but also helical movement (with rotation). Thus, it is beneficial to add a new parameter of “rotation” to the evaluation criteria of bull ejaculated sperm motility.
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spelling pubmed-61895692018-10-19 Reconsideration of the evaluation criteria for bull ejaculated sperm motility in the context of rotation YAMADA, Ayano SAKASE, Mitsuhiro FUKUSHIMA, Moriyuki HARAYAMA, Hiroshi J Reprod Dev Original Article Progressive movement of spermatozoa has conventionally been regarded as a good indicator of motility. However, bull spermatozoa exhibit two types of progressive movement: progressive/planar movement without rotation and progressive/helical movement with rotation. The aim of this study was to reconsider the evaluation criteria of bull ejaculated sperm motility in the context of rotation. Here, we compared the movement patterns of ejaculated spermatozoa with relatively high and low protein kinase A (PKA)-mediated signaling activities, because sperm motility is positively regulated by PKA-mediated signaling activities. We prepared sperm samples with high and low PKA-mediated signaling activities by suspending spermatozoa in media containing either the stimulator (NaHCO(3)) or inhibitor (KH-7) of adenylyl cyclase 10, and we then investigated movement patterns and relative velocities using a microscopic high-speed camera and recording system. In the control medium without NaHCO(3) and KH-7, most spermatozoa exhibited round/planar movement without rotation and asymmetrical bends in the principal pieces. NaHCO(3) significantly promoted changes in movement patterns from round/planar movement to progressive/planar movement (without rotation) as well as symmetrization of flagellar bends and increased relative velocities. KH-7 significantly increased spermatozoa exhibiting progressive/helical movement (with rotation), decreased relative velocities, and symmetrized flagellar bends with a reduction in their size. These indicate that progressive/planar movement (without rotation) and fast movement characterize the movement patterns of bull ejaculated spermatozoa with high PKA-mediated signaling activities. A sign of reduced PKA-mediated signaling activity is not only slow movement but also helical movement (with rotation). Thus, it is beneficial to add a new parameter of “rotation” to the evaluation criteria of bull ejaculated sperm motility. The Society for Reproduction and Development 2018-06-27 2018-10 /pmc/articles/PMC6189569/ /pubmed/29952339 http://dx.doi.org/10.1262/jrd.2018-036 Text en ©2018 Society for Reproduction and Development This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Original Article
YAMADA, Ayano
SAKASE, Mitsuhiro
FUKUSHIMA, Moriyuki
HARAYAMA, Hiroshi
Reconsideration of the evaluation criteria for bull ejaculated sperm motility in the context of rotation
title Reconsideration of the evaluation criteria for bull ejaculated sperm motility in the context of rotation
title_full Reconsideration of the evaluation criteria for bull ejaculated sperm motility in the context of rotation
title_fullStr Reconsideration of the evaluation criteria for bull ejaculated sperm motility in the context of rotation
title_full_unstemmed Reconsideration of the evaluation criteria for bull ejaculated sperm motility in the context of rotation
title_short Reconsideration of the evaluation criteria for bull ejaculated sperm motility in the context of rotation
title_sort reconsideration of the evaluation criteria for bull ejaculated sperm motility in the context of rotation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189569/
https://www.ncbi.nlm.nih.gov/pubmed/29952339
http://dx.doi.org/10.1262/jrd.2018-036
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