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Comparison of the microdrop and minimum volume cooling methods for vitrification of porcine in vitro-produced zygotes and blastocysts after equilibration in low concentrations of cryoprotectant agents
We compared the efficacy of the microdrop and minimum volume cooling (MVC) methods for the vitrification of in vitro-produced porcine zygotes and blastocysts after equilibration in low concentrations of cryoprotectant agents. Zygotes and blastocysts were equilibrated in 2% (v/v) ethylene glycol and...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Society for Reproduction and Development
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189571/ https://www.ncbi.nlm.nih.gov/pubmed/30101829 http://dx.doi.org/10.1262/jrd.2018-047 |
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author | NGUYEN, Van Khanh VU, Huong Thi Thu NGUYEN, Huong Thi QUAN, Huu Xuan PHAM, Lan Doan KIKUCHI, Kazuhiro NGUYEN, Son Thanh SOMFAI, Tamas |
author_facet | NGUYEN, Van Khanh VU, Huong Thi Thu NGUYEN, Huong Thi QUAN, Huu Xuan PHAM, Lan Doan KIKUCHI, Kazuhiro NGUYEN, Son Thanh SOMFAI, Tamas |
author_sort | NGUYEN, Van Khanh |
collection | PubMed |
description | We compared the efficacy of the microdrop and minimum volume cooling (MVC) methods for the vitrification of in vitro-produced porcine zygotes and blastocysts after equilibration in low concentrations of cryoprotectant agents. Zygotes and blastocysts were equilibrated in 2% (v/v) ethylene glycol and 2% (v/v) propylene glycol for 13–15 min. Then, they were vitrified in a medium comprised of 17.5% ethylene glycol, 17.5% propylene glycol, 0.3 M sucrose, and 50 mg/ml polyvinylpyrrolidone either by either dropping them directly into liquid nitrogen (microdrop method) or placing them on Cryotop sheets in a minimum volume of medium and plunging into liquid nitrogen (MVC method). Both zygotes and blastocysts were successfully vitrified. For the vitrification of zygotes, the MVC and microdrop methods were equally effective; however, for blastocyst vitrification, MVC was superior. For both methods, the vitrification of zygotes produced higher-quality embryos than the vitrification of blastocysts. |
format | Online Article Text |
id | pubmed-6189571 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Society for Reproduction and Development |
record_format | MEDLINE/PubMed |
spelling | pubmed-61895712018-10-19 Comparison of the microdrop and minimum volume cooling methods for vitrification of porcine in vitro-produced zygotes and blastocysts after equilibration in low concentrations of cryoprotectant agents NGUYEN, Van Khanh VU, Huong Thi Thu NGUYEN, Huong Thi QUAN, Huu Xuan PHAM, Lan Doan KIKUCHI, Kazuhiro NGUYEN, Son Thanh SOMFAI, Tamas J Reprod Dev Technology Report We compared the efficacy of the microdrop and minimum volume cooling (MVC) methods for the vitrification of in vitro-produced porcine zygotes and blastocysts after equilibration in low concentrations of cryoprotectant agents. Zygotes and blastocysts were equilibrated in 2% (v/v) ethylene glycol and 2% (v/v) propylene glycol for 13–15 min. Then, they were vitrified in a medium comprised of 17.5% ethylene glycol, 17.5% propylene glycol, 0.3 M sucrose, and 50 mg/ml polyvinylpyrrolidone either by either dropping them directly into liquid nitrogen (microdrop method) or placing them on Cryotop sheets in a minimum volume of medium and plunging into liquid nitrogen (MVC method). Both zygotes and blastocysts were successfully vitrified. For the vitrification of zygotes, the MVC and microdrop methods were equally effective; however, for blastocyst vitrification, MVC was superior. For both methods, the vitrification of zygotes produced higher-quality embryos than the vitrification of blastocysts. The Society for Reproduction and Development 2018-08-10 2018-10 /pmc/articles/PMC6189571/ /pubmed/30101829 http://dx.doi.org/10.1262/jrd.2018-047 Text en ©2018 Society for Reproduction and Development This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Technology Report NGUYEN, Van Khanh VU, Huong Thi Thu NGUYEN, Huong Thi QUAN, Huu Xuan PHAM, Lan Doan KIKUCHI, Kazuhiro NGUYEN, Son Thanh SOMFAI, Tamas Comparison of the microdrop and minimum volume cooling methods for vitrification of porcine in vitro-produced zygotes and blastocysts after equilibration in low concentrations of cryoprotectant agents |
title | Comparison of the microdrop and minimum volume cooling methods for vitrification of porcine in vitro-produced zygotes and blastocysts after equilibration in low
concentrations of cryoprotectant agents |
title_full | Comparison of the microdrop and minimum volume cooling methods for vitrification of porcine in vitro-produced zygotes and blastocysts after equilibration in low
concentrations of cryoprotectant agents |
title_fullStr | Comparison of the microdrop and minimum volume cooling methods for vitrification of porcine in vitro-produced zygotes and blastocysts after equilibration in low
concentrations of cryoprotectant agents |
title_full_unstemmed | Comparison of the microdrop and minimum volume cooling methods for vitrification of porcine in vitro-produced zygotes and blastocysts after equilibration in low
concentrations of cryoprotectant agents |
title_short | Comparison of the microdrop and minimum volume cooling methods for vitrification of porcine in vitro-produced zygotes and blastocysts after equilibration in low
concentrations of cryoprotectant agents |
title_sort | comparison of the microdrop and minimum volume cooling methods for vitrification of porcine in vitro-produced zygotes and blastocysts after equilibration in low
concentrations of cryoprotectant agents |
topic | Technology Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189571/ https://www.ncbi.nlm.nih.gov/pubmed/30101829 http://dx.doi.org/10.1262/jrd.2018-047 |
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