Cargando…
Protective Effects of Silymarin and Silibinin against DNA Damage in Human Blood Cells
Silymarin (SM), a standardized extract derived from Silybum marianum (L.) Gaertn, is primarily composed of flavonolignans, with silibinin (SB) as its major active constituent. The present study aimed to evaluate the antigenotoxic activities of SM and SB using the alkaline comet assay in whole blood...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189666/ https://www.ncbi.nlm.nih.gov/pubmed/30370304 http://dx.doi.org/10.1155/2018/6056948 |
_version_ | 1783363405047922688 |
---|---|
author | Fernandes Veloso Borges, Flávio Ribeiro e Silva, Carolina Moreira Goes, Wanessa Ribeiro Godoy, Fernanda Craveiro Franco, Fernanda Hollanda Véras, Jefferson Luiz Cardoso Bailão, Elisa Flávia de Melo e Silva, Daniela Gomes Cardoso, Clever Divino da Cruz, Aparecido Chen-Chen, Lee |
author_facet | Fernandes Veloso Borges, Flávio Ribeiro e Silva, Carolina Moreira Goes, Wanessa Ribeiro Godoy, Fernanda Craveiro Franco, Fernanda Hollanda Véras, Jefferson Luiz Cardoso Bailão, Elisa Flávia de Melo e Silva, Daniela Gomes Cardoso, Clever Divino da Cruz, Aparecido Chen-Chen, Lee |
author_sort | Fernandes Veloso Borges, Flávio |
collection | PubMed |
description | Silymarin (SM), a standardized extract derived from Silybum marianum (L.) Gaertn, is primarily composed of flavonolignans, with silibinin (SB) as its major active constituent. The present study aimed to evaluate the antigenotoxic activities of SM and SB using the alkaline comet assay in whole blood cells and to assess their effects on the expression of genes associated with carcinogenesis and chemopreventive processes. Different concentrations of SM or SB (1.0, 2.5, 5.0, and 7.5 mg/ml) were used in combination with the DNA damage-inducing agent methyl methanesulfonate (MMS, 800 μM) to evaluate their genoprotective potential. To investigate the role of SM and SB in modulating gene expression, we performed quantitative real-time PCR (qRT-PCR) analysis of five genes that are known to be involved in DNA damage, carcinogenesis, and/or chemopreventive mechanisms. Treatment with SM or SB was found to significantly reduce the genotoxicity of MMS, upregulate the expression of PTEN and BCL2, and downregulate the expression of BAX and ABL1. We observed no significant changes in ETV6 expression levels following treatment with SM or SB. In conclusion, both SM and SB exerted antigenotoxic activities and modulated the expression of genes related to cell protection against DNA damage. |
format | Online Article Text |
id | pubmed-6189666 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-61896662018-10-28 Protective Effects of Silymarin and Silibinin against DNA Damage in Human Blood Cells Fernandes Veloso Borges, Flávio Ribeiro e Silva, Carolina Moreira Goes, Wanessa Ribeiro Godoy, Fernanda Craveiro Franco, Fernanda Hollanda Véras, Jefferson Luiz Cardoso Bailão, Elisa Flávia de Melo e Silva, Daniela Gomes Cardoso, Clever Divino da Cruz, Aparecido Chen-Chen, Lee Biomed Res Int Research Article Silymarin (SM), a standardized extract derived from Silybum marianum (L.) Gaertn, is primarily composed of flavonolignans, with silibinin (SB) as its major active constituent. The present study aimed to evaluate the antigenotoxic activities of SM and SB using the alkaline comet assay in whole blood cells and to assess their effects on the expression of genes associated with carcinogenesis and chemopreventive processes. Different concentrations of SM or SB (1.0, 2.5, 5.0, and 7.5 mg/ml) were used in combination with the DNA damage-inducing agent methyl methanesulfonate (MMS, 800 μM) to evaluate their genoprotective potential. To investigate the role of SM and SB in modulating gene expression, we performed quantitative real-time PCR (qRT-PCR) analysis of five genes that are known to be involved in DNA damage, carcinogenesis, and/or chemopreventive mechanisms. Treatment with SM or SB was found to significantly reduce the genotoxicity of MMS, upregulate the expression of PTEN and BCL2, and downregulate the expression of BAX and ABL1. We observed no significant changes in ETV6 expression levels following treatment with SM or SB. In conclusion, both SM and SB exerted antigenotoxic activities and modulated the expression of genes related to cell protection against DNA damage. Hindawi 2018-10-02 /pmc/articles/PMC6189666/ /pubmed/30370304 http://dx.doi.org/10.1155/2018/6056948 Text en Copyright © 2018 Flávio Fernandes Veloso Borges et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Fernandes Veloso Borges, Flávio Ribeiro e Silva, Carolina Moreira Goes, Wanessa Ribeiro Godoy, Fernanda Craveiro Franco, Fernanda Hollanda Véras, Jefferson Luiz Cardoso Bailão, Elisa Flávia de Melo e Silva, Daniela Gomes Cardoso, Clever Divino da Cruz, Aparecido Chen-Chen, Lee Protective Effects of Silymarin and Silibinin against DNA Damage in Human Blood Cells |
title | Protective Effects of Silymarin and Silibinin against DNA Damage in Human Blood Cells |
title_full | Protective Effects of Silymarin and Silibinin against DNA Damage in Human Blood Cells |
title_fullStr | Protective Effects of Silymarin and Silibinin against DNA Damage in Human Blood Cells |
title_full_unstemmed | Protective Effects of Silymarin and Silibinin against DNA Damage in Human Blood Cells |
title_short | Protective Effects of Silymarin and Silibinin against DNA Damage in Human Blood Cells |
title_sort | protective effects of silymarin and silibinin against dna damage in human blood cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189666/ https://www.ncbi.nlm.nih.gov/pubmed/30370304 http://dx.doi.org/10.1155/2018/6056948 |
work_keys_str_mv | AT fernandesvelosoborgesflavio protectiveeffectsofsilymarinandsilibininagainstdnadamageinhumanbloodcells AT ribeiroesilvacarolina protectiveeffectsofsilymarinandsilibininagainstdnadamageinhumanbloodcells AT moreiragoeswanessa protectiveeffectsofsilymarinandsilibininagainstdnadamageinhumanbloodcells AT ribeirogodoyfernanda protectiveeffectsofsilymarinandsilibininagainstdnadamageinhumanbloodcells AT craveirofrancofernanda protectiveeffectsofsilymarinandsilibininagainstdnadamageinhumanbloodcells AT hollandaverasjefferson protectiveeffectsofsilymarinandsilibininagainstdnadamageinhumanbloodcells AT luizcardosobailaoelisaflavia protectiveeffectsofsilymarinandsilibininagainstdnadamageinhumanbloodcells AT demeloesilvadaniela protectiveeffectsofsilymarinandsilibininagainstdnadamageinhumanbloodcells AT gomescardosoclever protectiveeffectsofsilymarinandsilibininagainstdnadamageinhumanbloodcells AT divinodacruzaparecido protectiveeffectsofsilymarinandsilibininagainstdnadamageinhumanbloodcells AT chenchenlee protectiveeffectsofsilymarinandsilibininagainstdnadamageinhumanbloodcells |