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Cell Migration According to Shape of Graphene Oxide Micropatterns

Photolithography is a unique process that can effectively manufacture micro/nano-sized patterns on various substrates. On the other hand, the meniscus-dragging deposition (MDD) process can produce a uniform surface of the substrate. Graphene oxide (GO) is the oxidized form of graphene that has high...

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Autores principales: Kim, Sung Eun, Kim, Min Sung, Shin, Yong Cheol, Eom, Seong Un, Lee, Jong Ho, Shin, Dong-Myeong, Hong, Suck Won, Kim, Bongju, Park, Jong-Chul, Shin, Bo Sung, Lim, Dohyung, Han, Dong-Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189709/
https://www.ncbi.nlm.nih.gov/pubmed/30404359
http://dx.doi.org/10.3390/mi7100186
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author Kim, Sung Eun
Kim, Min Sung
Shin, Yong Cheol
Eom, Seong Un
Lee, Jong Ho
Shin, Dong-Myeong
Hong, Suck Won
Kim, Bongju
Park, Jong-Chul
Shin, Bo Sung
Lim, Dohyung
Han, Dong-Wook
author_facet Kim, Sung Eun
Kim, Min Sung
Shin, Yong Cheol
Eom, Seong Un
Lee, Jong Ho
Shin, Dong-Myeong
Hong, Suck Won
Kim, Bongju
Park, Jong-Chul
Shin, Bo Sung
Lim, Dohyung
Han, Dong-Wook
author_sort Kim, Sung Eun
collection PubMed
description Photolithography is a unique process that can effectively manufacture micro/nano-sized patterns on various substrates. On the other hand, the meniscus-dragging deposition (MDD) process can produce a uniform surface of the substrate. Graphene oxide (GO) is the oxidized form of graphene that has high hydrophilicity and protein absorption. It is widely used in biomedical fields such as drug delivery, regenerative medicine, and tissue engineering. Herein, we fabricated uniform GO micropatterns via MDD and photolithography. The physicochemical properties of the GO micropatterns were characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM), and Raman spectroscopy. Furthermore, cell migration on the GO micropatterns was investigated, and the difference in cell migration on triangle and square GO micropatterns was examined for their effects on cell migration. Our results demonstrated that the GO micropatterns with a desired shape can be finely fabricated via MDD and photolithography. Moreover, it was revealed that the shape of GO micropatterns plays a crucial role in cell migration distance, speed, and directionality. Therefore, our findings suggest that the GO micropatterns can serve as a promising biofunctional platform and cell-guiding substrate for applications to bioelectric devices, cell-on-a-chip, and tissue engineering scaffolds.
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spelling pubmed-61897092018-11-01 Cell Migration According to Shape of Graphene Oxide Micropatterns Kim, Sung Eun Kim, Min Sung Shin, Yong Cheol Eom, Seong Un Lee, Jong Ho Shin, Dong-Myeong Hong, Suck Won Kim, Bongju Park, Jong-Chul Shin, Bo Sung Lim, Dohyung Han, Dong-Wook Micromachines (Basel) Article Photolithography is a unique process that can effectively manufacture micro/nano-sized patterns on various substrates. On the other hand, the meniscus-dragging deposition (MDD) process can produce a uniform surface of the substrate. Graphene oxide (GO) is the oxidized form of graphene that has high hydrophilicity and protein absorption. It is widely used in biomedical fields such as drug delivery, regenerative medicine, and tissue engineering. Herein, we fabricated uniform GO micropatterns via MDD and photolithography. The physicochemical properties of the GO micropatterns were characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM), and Raman spectroscopy. Furthermore, cell migration on the GO micropatterns was investigated, and the difference in cell migration on triangle and square GO micropatterns was examined for their effects on cell migration. Our results demonstrated that the GO micropatterns with a desired shape can be finely fabricated via MDD and photolithography. Moreover, it was revealed that the shape of GO micropatterns plays a crucial role in cell migration distance, speed, and directionality. Therefore, our findings suggest that the GO micropatterns can serve as a promising biofunctional platform and cell-guiding substrate for applications to bioelectric devices, cell-on-a-chip, and tissue engineering scaffolds. MDPI 2016-10-14 /pmc/articles/PMC6189709/ /pubmed/30404359 http://dx.doi.org/10.3390/mi7100186 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Sung Eun
Kim, Min Sung
Shin, Yong Cheol
Eom, Seong Un
Lee, Jong Ho
Shin, Dong-Myeong
Hong, Suck Won
Kim, Bongju
Park, Jong-Chul
Shin, Bo Sung
Lim, Dohyung
Han, Dong-Wook
Cell Migration According to Shape of Graphene Oxide Micropatterns
title Cell Migration According to Shape of Graphene Oxide Micropatterns
title_full Cell Migration According to Shape of Graphene Oxide Micropatterns
title_fullStr Cell Migration According to Shape of Graphene Oxide Micropatterns
title_full_unstemmed Cell Migration According to Shape of Graphene Oxide Micropatterns
title_short Cell Migration According to Shape of Graphene Oxide Micropatterns
title_sort cell migration according to shape of graphene oxide micropatterns
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189709/
https://www.ncbi.nlm.nih.gov/pubmed/30404359
http://dx.doi.org/10.3390/mi7100186
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