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Integration of an Optical Ring Resonator Biosensor into a Self-Contained Microfluidic Cartridge with Active, Single-Shot Micropumps

While there have been huge advances in the field of biosensors during the last decade, their integration into a microfluidic environment avoiding external tubing and pumping is still neglected. Herein, we show a new microfluidic design that integrates multiple reservoirs for reagent storage and sing...

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Autores principales: Geidel, Sascha, Peransi Llopis, Sergio, Rodrigo, Manuel, de Diego-Castilla, Graciela, Sousa, Antonio, Nestler, Jörg, Otto, Thomas, Gessner, Thomas, Parro, Victor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189757/
https://www.ncbi.nlm.nih.gov/pubmed/30404337
http://dx.doi.org/10.3390/mi7090153
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author Geidel, Sascha
Peransi Llopis, Sergio
Rodrigo, Manuel
de Diego-Castilla, Graciela
Sousa, Antonio
Nestler, Jörg
Otto, Thomas
Gessner, Thomas
Parro, Victor
author_facet Geidel, Sascha
Peransi Llopis, Sergio
Rodrigo, Manuel
de Diego-Castilla, Graciela
Sousa, Antonio
Nestler, Jörg
Otto, Thomas
Gessner, Thomas
Parro, Victor
author_sort Geidel, Sascha
collection PubMed
description While there have been huge advances in the field of biosensors during the last decade, their integration into a microfluidic environment avoiding external tubing and pumping is still neglected. Herein, we show a new microfluidic design that integrates multiple reservoirs for reagent storage and single-use electrochemical pumps for time-controlled delivery of the liquids. The cartridge has been tested and validated with a silicon nitride-based photonic biosensor incorporating multiple optical ring resonators as sensing elements and an immunoassay as a potential target application. Based on experimental results obtained with a demonstration model, subcomponents were designed and existing protocols were adapted. The newly-designed microfluidic cartridges and photonic sensors were separately characterized on a technical basis and performed well. Afterwards, the sensor was functionalized for a protein detection. The microfluidic cartridge was loaded with the necessary assay reagents. The integrated pumps were programmed to drive the single process steps of an immunoassay. The prototype worked selectively, but only with a low sensitivity. Further work must be carried out to optimize biofunctionalization of the optical ring resonators and to have a more suitable flow velocity progression to enhance the system’s reproducibility.
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spelling pubmed-61897572018-11-01 Integration of an Optical Ring Resonator Biosensor into a Self-Contained Microfluidic Cartridge with Active, Single-Shot Micropumps Geidel, Sascha Peransi Llopis, Sergio Rodrigo, Manuel de Diego-Castilla, Graciela Sousa, Antonio Nestler, Jörg Otto, Thomas Gessner, Thomas Parro, Victor Micromachines (Basel) Article While there have been huge advances in the field of biosensors during the last decade, their integration into a microfluidic environment avoiding external tubing and pumping is still neglected. Herein, we show a new microfluidic design that integrates multiple reservoirs for reagent storage and single-use electrochemical pumps for time-controlled delivery of the liquids. The cartridge has been tested and validated with a silicon nitride-based photonic biosensor incorporating multiple optical ring resonators as sensing elements and an immunoassay as a potential target application. Based on experimental results obtained with a demonstration model, subcomponents were designed and existing protocols were adapted. The newly-designed microfluidic cartridges and photonic sensors were separately characterized on a technical basis and performed well. Afterwards, the sensor was functionalized for a protein detection. The microfluidic cartridge was loaded with the necessary assay reagents. The integrated pumps were programmed to drive the single process steps of an immunoassay. The prototype worked selectively, but only with a low sensitivity. Further work must be carried out to optimize biofunctionalization of the optical ring resonators and to have a more suitable flow velocity progression to enhance the system’s reproducibility. MDPI 2016-09-13 /pmc/articles/PMC6189757/ /pubmed/30404337 http://dx.doi.org/10.3390/mi7090153 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Geidel, Sascha
Peransi Llopis, Sergio
Rodrigo, Manuel
de Diego-Castilla, Graciela
Sousa, Antonio
Nestler, Jörg
Otto, Thomas
Gessner, Thomas
Parro, Victor
Integration of an Optical Ring Resonator Biosensor into a Self-Contained Microfluidic Cartridge with Active, Single-Shot Micropumps
title Integration of an Optical Ring Resonator Biosensor into a Self-Contained Microfluidic Cartridge with Active, Single-Shot Micropumps
title_full Integration of an Optical Ring Resonator Biosensor into a Self-Contained Microfluidic Cartridge with Active, Single-Shot Micropumps
title_fullStr Integration of an Optical Ring Resonator Biosensor into a Self-Contained Microfluidic Cartridge with Active, Single-Shot Micropumps
title_full_unstemmed Integration of an Optical Ring Resonator Biosensor into a Self-Contained Microfluidic Cartridge with Active, Single-Shot Micropumps
title_short Integration of an Optical Ring Resonator Biosensor into a Self-Contained Microfluidic Cartridge with Active, Single-Shot Micropumps
title_sort integration of an optical ring resonator biosensor into a self-contained microfluidic cartridge with active, single-shot micropumps
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189757/
https://www.ncbi.nlm.nih.gov/pubmed/30404337
http://dx.doi.org/10.3390/mi7090153
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