Development of the Electric Equivalent Model for the Cytoplasmic Microinjection of Small Adherent Cells

A novel approach utilizing current feedback for the cytoplasmic microinjection of biological cells is proposed. In order to realize the cytoplasmic microinjection on small adherent cells (diameter < 30 μm and thickness < 10 μm), an electrical model is built and analyzed according to the electr...

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Autores principales: Chan, Florence Hiu Ling, Yang, Runhuai, Lai, King Wai Chiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189767/
https://www.ncbi.nlm.nih.gov/pubmed/30400407
http://dx.doi.org/10.3390/mi8070216
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author Chan, Florence Hiu Ling
Yang, Runhuai
Lai, King Wai Chiu
author_facet Chan, Florence Hiu Ling
Yang, Runhuai
Lai, King Wai Chiu
author_sort Chan, Florence Hiu Ling
collection PubMed
description A novel approach utilizing current feedback for the cytoplasmic microinjection of biological cells is proposed. In order to realize the cytoplasmic microinjection on small adherent cells (diameter < 30 μm and thickness < 10 μm), an electrical model is built and analyzed according to the electrochemical properties of target cells. In this study, we have verified the effectiveness of the current measurement for monitoring the injection process and the study of ion channel activities for verifying the cell viability of the cells after the microinjection.
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spelling pubmed-61897672018-11-01 Development of the Electric Equivalent Model for the Cytoplasmic Microinjection of Small Adherent Cells Chan, Florence Hiu Ling Yang, Runhuai Lai, King Wai Chiu Micromachines (Basel) Article A novel approach utilizing current feedback for the cytoplasmic microinjection of biological cells is proposed. In order to realize the cytoplasmic microinjection on small adherent cells (diameter < 30 μm and thickness < 10 μm), an electrical model is built and analyzed according to the electrochemical properties of target cells. In this study, we have verified the effectiveness of the current measurement for monitoring the injection process and the study of ion channel activities for verifying the cell viability of the cells after the microinjection. MDPI 2017-07-08 /pmc/articles/PMC6189767/ /pubmed/30400407 http://dx.doi.org/10.3390/mi8070216 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chan, Florence Hiu Ling
Yang, Runhuai
Lai, King Wai Chiu
Development of the Electric Equivalent Model for the Cytoplasmic Microinjection of Small Adherent Cells
title Development of the Electric Equivalent Model for the Cytoplasmic Microinjection of Small Adherent Cells
title_full Development of the Electric Equivalent Model for the Cytoplasmic Microinjection of Small Adherent Cells
title_fullStr Development of the Electric Equivalent Model for the Cytoplasmic Microinjection of Small Adherent Cells
title_full_unstemmed Development of the Electric Equivalent Model for the Cytoplasmic Microinjection of Small Adherent Cells
title_short Development of the Electric Equivalent Model for the Cytoplasmic Microinjection of Small Adherent Cells
title_sort development of the electric equivalent model for the cytoplasmic microinjection of small adherent cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189767/
https://www.ncbi.nlm.nih.gov/pubmed/30400407
http://dx.doi.org/10.3390/mi8070216
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AT yangrunhuai developmentoftheelectricequivalentmodelforthecytoplasmicmicroinjectionofsmalladherentcells
AT laikingwaichiu developmentoftheelectricequivalentmodelforthecytoplasmicmicroinjectionofsmalladherentcells

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