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Microfluidic Paper-Based Sample Concentration Using Ion Concentration Polarization with Smartphone Detection

A simple method for microfluidic paper-based sample concentration using ion concentration polarization (ICP) with smartphone detection is developed. The concise and low-cost microfluidic paper-based ICP analytical device, which consists of a black backing layer, a nitrocellulose membrane, and two ab...

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Detalles Bibliográficos
Autores principales: Li, Xue, Niu, Yanan, Chen, Yunyi, Wu, Di, Yi, Long, Qiu, Xianbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189865/
https://www.ncbi.nlm.nih.gov/pubmed/30404372
http://dx.doi.org/10.3390/mi7110199
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author Li, Xue
Niu, Yanan
Chen, Yunyi
Wu, Di
Yi, Long
Qiu, Xianbo
author_facet Li, Xue
Niu, Yanan
Chen, Yunyi
Wu, Di
Yi, Long
Qiu, Xianbo
author_sort Li, Xue
collection PubMed
description A simple method for microfluidic paper-based sample concentration using ion concentration polarization (ICP) with smartphone detection is developed. The concise and low-cost microfluidic paper-based ICP analytical device, which consists of a black backing layer, a nitrocellulose membrane, and two absorbent pads, is fabricated with the simple lamination method which is widely used for lateral flow strips. Sample concentration on the nitrocellulose membrane is monitored in real time by a smartphone whose camera is used to collect the fluorescence images from the ICP device. A custom image processing algorithm running on the smartphone is used to track the concentrated sample and obtain its fluorescence signal intensity for quantitative analysis. Two different methods for Nafion coating are evaluated and their performances are compared. The characteristics of the ICP analytical device especially with intentionally adjusted physical properties are fully evaluated to optimize its performance as well as to extend its potential applications. Experimental results show that significant concentration enhancement with fluorescence dye sample is obtained with the developed ICP device when a fast depletion of fluorescent dye is observed. The platform based on the simply laminated ICP device with smartphone detection is desired for point-of-care testing in settings with poor resources.
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spelling pubmed-61898652018-11-01 Microfluidic Paper-Based Sample Concentration Using Ion Concentration Polarization with Smartphone Detection Li, Xue Niu, Yanan Chen, Yunyi Wu, Di Yi, Long Qiu, Xianbo Micromachines (Basel) Article A simple method for microfluidic paper-based sample concentration using ion concentration polarization (ICP) with smartphone detection is developed. The concise and low-cost microfluidic paper-based ICP analytical device, which consists of a black backing layer, a nitrocellulose membrane, and two absorbent pads, is fabricated with the simple lamination method which is widely used for lateral flow strips. Sample concentration on the nitrocellulose membrane is monitored in real time by a smartphone whose camera is used to collect the fluorescence images from the ICP device. A custom image processing algorithm running on the smartphone is used to track the concentrated sample and obtain its fluorescence signal intensity for quantitative analysis. Two different methods for Nafion coating are evaluated and their performances are compared. The characteristics of the ICP analytical device especially with intentionally adjusted physical properties are fully evaluated to optimize its performance as well as to extend its potential applications. Experimental results show that significant concentration enhancement with fluorescence dye sample is obtained with the developed ICP device when a fast depletion of fluorescent dye is observed. The platform based on the simply laminated ICP device with smartphone detection is desired for point-of-care testing in settings with poor resources. MDPI 2016-11-04 /pmc/articles/PMC6189865/ /pubmed/30404372 http://dx.doi.org/10.3390/mi7110199 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Xue
Niu, Yanan
Chen, Yunyi
Wu, Di
Yi, Long
Qiu, Xianbo
Microfluidic Paper-Based Sample Concentration Using Ion Concentration Polarization with Smartphone Detection
title Microfluidic Paper-Based Sample Concentration Using Ion Concentration Polarization with Smartphone Detection
title_full Microfluidic Paper-Based Sample Concentration Using Ion Concentration Polarization with Smartphone Detection
title_fullStr Microfluidic Paper-Based Sample Concentration Using Ion Concentration Polarization with Smartphone Detection
title_full_unstemmed Microfluidic Paper-Based Sample Concentration Using Ion Concentration Polarization with Smartphone Detection
title_short Microfluidic Paper-Based Sample Concentration Using Ion Concentration Polarization with Smartphone Detection
title_sort microfluidic paper-based sample concentration using ion concentration polarization with smartphone detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189865/
https://www.ncbi.nlm.nih.gov/pubmed/30404372
http://dx.doi.org/10.3390/mi7110199
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