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Size-Adjustable Microdroplets Generation Based on Microinjection

Microinjection is a promising tool for microdroplet generation, while the microinjection for microdroplets generation still remains a challenging issue due to the Laplace pressure at the micropipette opening. Here, we apply a simple and robust substrate-contacting microinjection method to microdropl...

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Autores principales: Li, Shibao, Zheng, Deyin, Li, Na, Wang, Xuefeng, Liu, Yaowei, Sun, Mingzhu, Zhao, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189902/
http://dx.doi.org/10.3390/mi8030088
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author Li, Shibao
Zheng, Deyin
Li, Na
Wang, Xuefeng
Liu, Yaowei
Sun, Mingzhu
Zhao, Xin
author_facet Li, Shibao
Zheng, Deyin
Li, Na
Wang, Xuefeng
Liu, Yaowei
Sun, Mingzhu
Zhao, Xin
author_sort Li, Shibao
collection PubMed
description Microinjection is a promising tool for microdroplet generation, while the microinjection for microdroplets generation still remains a challenging issue due to the Laplace pressure at the micropipette opening. Here, we apply a simple and robust substrate-contacting microinjection method to microdroplet generation, presenting a size-adjustable microdroplets generation method based on a critical injection (CI) model. Firstly, the micropipette is adjusted to a preset injection pressure. Secondly, the micropipette is moved down to contact the substrate, then, the Laplace pressure in the droplet is no longer relevant and the liquid flows out in time. The liquid constantly flows out until the micropipette is lifted, ending the substrate-contacting situation, which results in the recovery of the Laplace pressure at the micropipette opening, and the liquid injection is terminated. We carry out five groups of experiments whereupon 1600 images are captured within each group and the microdroplet radius of each image is detected. Then we determine the relationship among microdroplet radius, radius at the micropipette opening, time, and pressure, and, two more experiments are conducted to verify the relationship. To verify the effectiveness of the substrate-contacting method and the relationship, we conducted two experiments with six desired microdroplet radii are set in each experiment, by adjusting the injection time with a given pressure, and adjusting the injection pressure with a given time. Then, six arrays of microdroplets are obtained in each experiment. The results of the experiments show that the standard errors of the microdroplet radii are less than 2% and the experimental errors fall in the range of ±5%. The average operating speed is 20 microdroplets/min and the minimum radius of the microdroplets is 25 μm. This method has a simple experimental setup that enables easy manipulation and lower cost.
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spelling pubmed-61899022018-11-01 Size-Adjustable Microdroplets Generation Based on Microinjection Li, Shibao Zheng, Deyin Li, Na Wang, Xuefeng Liu, Yaowei Sun, Mingzhu Zhao, Xin Micromachines (Basel) Article Microinjection is a promising tool for microdroplet generation, while the microinjection for microdroplets generation still remains a challenging issue due to the Laplace pressure at the micropipette opening. Here, we apply a simple and robust substrate-contacting microinjection method to microdroplet generation, presenting a size-adjustable microdroplets generation method based on a critical injection (CI) model. Firstly, the micropipette is adjusted to a preset injection pressure. Secondly, the micropipette is moved down to contact the substrate, then, the Laplace pressure in the droplet is no longer relevant and the liquid flows out in time. The liquid constantly flows out until the micropipette is lifted, ending the substrate-contacting situation, which results in the recovery of the Laplace pressure at the micropipette opening, and the liquid injection is terminated. We carry out five groups of experiments whereupon 1600 images are captured within each group and the microdroplet radius of each image is detected. Then we determine the relationship among microdroplet radius, radius at the micropipette opening, time, and pressure, and, two more experiments are conducted to verify the relationship. To verify the effectiveness of the substrate-contacting method and the relationship, we conducted two experiments with six desired microdroplet radii are set in each experiment, by adjusting the injection time with a given pressure, and adjusting the injection pressure with a given time. Then, six arrays of microdroplets are obtained in each experiment. The results of the experiments show that the standard errors of the microdroplet radii are less than 2% and the experimental errors fall in the range of ±5%. The average operating speed is 20 microdroplets/min and the minimum radius of the microdroplets is 25 μm. This method has a simple experimental setup that enables easy manipulation and lower cost. MDPI 2017-03-12 /pmc/articles/PMC6189902/ http://dx.doi.org/10.3390/mi8030088 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Shibao
Zheng, Deyin
Li, Na
Wang, Xuefeng
Liu, Yaowei
Sun, Mingzhu
Zhao, Xin
Size-Adjustable Microdroplets Generation Based on Microinjection
title Size-Adjustable Microdroplets Generation Based on Microinjection
title_full Size-Adjustable Microdroplets Generation Based on Microinjection
title_fullStr Size-Adjustable Microdroplets Generation Based on Microinjection
title_full_unstemmed Size-Adjustable Microdroplets Generation Based on Microinjection
title_short Size-Adjustable Microdroplets Generation Based on Microinjection
title_sort size-adjustable microdroplets generation based on microinjection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189902/
http://dx.doi.org/10.3390/mi8030088
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