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High-Resolution Microfluidic Paper-Based Analytical Devices for Sub-Microliter Sample Analysis
This work demonstrates the fabrication of microfluidic paper-based analytical devices (µPADs) suitable for the analysis of sub-microliter sample volumes. The wax-printing approach widely used for the patterning of paper substrates has been adapted to obtain high-resolution microfluidic structures pa...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189915/ https://www.ncbi.nlm.nih.gov/pubmed/30404255 http://dx.doi.org/10.3390/mi7050080 |
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author | Tenda, Keisuke Ota, Riki Yamada, Kentaro Henares, Terence G. Suzuki, Koji Citterio, Daniel |
author_facet | Tenda, Keisuke Ota, Riki Yamada, Kentaro Henares, Terence G. Suzuki, Koji Citterio, Daniel |
author_sort | Tenda, Keisuke |
collection | PubMed |
description | This work demonstrates the fabrication of microfluidic paper-based analytical devices (µPADs) suitable for the analysis of sub-microliter sample volumes. The wax-printing approach widely used for the patterning of paper substrates has been adapted to obtain high-resolution microfluidic structures patterned in filter paper. This has been achieved by replacing the hot plate heating method conventionally used to melt printed wax features into paper by simple hot lamination. This patterning technique, in combination with the consideration of device geometry and the influence of cellulose fiber direction in filter paper, led to a model µPAD design with four microfluidic channels that can be filled with as low as 0.5 µL of liquid. Finally, the application to a colorimetric model assay targeting total protein concentrations is shown. Calibration curves for human serum albumin (HSA) were recorded from sub-microliter samples (0.8 µL), with tolerance against ±0.1 µL variations in the applied liquid volume. |
format | Online Article Text |
id | pubmed-6189915 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61899152018-11-01 High-Resolution Microfluidic Paper-Based Analytical Devices for Sub-Microliter Sample Analysis Tenda, Keisuke Ota, Riki Yamada, Kentaro Henares, Terence G. Suzuki, Koji Citterio, Daniel Micromachines (Basel) Article This work demonstrates the fabrication of microfluidic paper-based analytical devices (µPADs) suitable for the analysis of sub-microliter sample volumes. The wax-printing approach widely used for the patterning of paper substrates has been adapted to obtain high-resolution microfluidic structures patterned in filter paper. This has been achieved by replacing the hot plate heating method conventionally used to melt printed wax features into paper by simple hot lamination. This patterning technique, in combination with the consideration of device geometry and the influence of cellulose fiber direction in filter paper, led to a model µPAD design with four microfluidic channels that can be filled with as low as 0.5 µL of liquid. Finally, the application to a colorimetric model assay targeting total protein concentrations is shown. Calibration curves for human serum albumin (HSA) were recorded from sub-microliter samples (0.8 µL), with tolerance against ±0.1 µL variations in the applied liquid volume. MDPI 2016-05-02 /pmc/articles/PMC6189915/ /pubmed/30404255 http://dx.doi.org/10.3390/mi7050080 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tenda, Keisuke Ota, Riki Yamada, Kentaro Henares, Terence G. Suzuki, Koji Citterio, Daniel High-Resolution Microfluidic Paper-Based Analytical Devices for Sub-Microliter Sample Analysis |
title | High-Resolution Microfluidic Paper-Based Analytical Devices for Sub-Microliter Sample Analysis |
title_full | High-Resolution Microfluidic Paper-Based Analytical Devices for Sub-Microliter Sample Analysis |
title_fullStr | High-Resolution Microfluidic Paper-Based Analytical Devices for Sub-Microliter Sample Analysis |
title_full_unstemmed | High-Resolution Microfluidic Paper-Based Analytical Devices for Sub-Microliter Sample Analysis |
title_short | High-Resolution Microfluidic Paper-Based Analytical Devices for Sub-Microliter Sample Analysis |
title_sort | high-resolution microfluidic paper-based analytical devices for sub-microliter sample analysis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189915/ https://www.ncbi.nlm.nih.gov/pubmed/30404255 http://dx.doi.org/10.3390/mi7050080 |
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