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High-Resolution Microfluidic Paper-Based Analytical Devices for Sub-Microliter Sample Analysis

This work demonstrates the fabrication of microfluidic paper-based analytical devices (µPADs) suitable for the analysis of sub-microliter sample volumes. The wax-printing approach widely used for the patterning of paper substrates has been adapted to obtain high-resolution microfluidic structures pa...

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Autores principales: Tenda, Keisuke, Ota, Riki, Yamada, Kentaro, Henares, Terence G., Suzuki, Koji, Citterio, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189915/
https://www.ncbi.nlm.nih.gov/pubmed/30404255
http://dx.doi.org/10.3390/mi7050080
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author Tenda, Keisuke
Ota, Riki
Yamada, Kentaro
Henares, Terence G.
Suzuki, Koji
Citterio, Daniel
author_facet Tenda, Keisuke
Ota, Riki
Yamada, Kentaro
Henares, Terence G.
Suzuki, Koji
Citterio, Daniel
author_sort Tenda, Keisuke
collection PubMed
description This work demonstrates the fabrication of microfluidic paper-based analytical devices (µPADs) suitable for the analysis of sub-microliter sample volumes. The wax-printing approach widely used for the patterning of paper substrates has been adapted to obtain high-resolution microfluidic structures patterned in filter paper. This has been achieved by replacing the hot plate heating method conventionally used to melt printed wax features into paper by simple hot lamination. This patterning technique, in combination with the consideration of device geometry and the influence of cellulose fiber direction in filter paper, led to a model µPAD design with four microfluidic channels that can be filled with as low as 0.5 µL of liquid. Finally, the application to a colorimetric model assay targeting total protein concentrations is shown. Calibration curves for human serum albumin (HSA) were recorded from sub-microliter samples (0.8 µL), with tolerance against ±0.1 µL variations in the applied liquid volume.
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spelling pubmed-61899152018-11-01 High-Resolution Microfluidic Paper-Based Analytical Devices for Sub-Microliter Sample Analysis Tenda, Keisuke Ota, Riki Yamada, Kentaro Henares, Terence G. Suzuki, Koji Citterio, Daniel Micromachines (Basel) Article This work demonstrates the fabrication of microfluidic paper-based analytical devices (µPADs) suitable for the analysis of sub-microliter sample volumes. The wax-printing approach widely used for the patterning of paper substrates has been adapted to obtain high-resolution microfluidic structures patterned in filter paper. This has been achieved by replacing the hot plate heating method conventionally used to melt printed wax features into paper by simple hot lamination. This patterning technique, in combination with the consideration of device geometry and the influence of cellulose fiber direction in filter paper, led to a model µPAD design with four microfluidic channels that can be filled with as low as 0.5 µL of liquid. Finally, the application to a colorimetric model assay targeting total protein concentrations is shown. Calibration curves for human serum albumin (HSA) were recorded from sub-microliter samples (0.8 µL), with tolerance against ±0.1 µL variations in the applied liquid volume. MDPI 2016-05-02 /pmc/articles/PMC6189915/ /pubmed/30404255 http://dx.doi.org/10.3390/mi7050080 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tenda, Keisuke
Ota, Riki
Yamada, Kentaro
Henares, Terence G.
Suzuki, Koji
Citterio, Daniel
High-Resolution Microfluidic Paper-Based Analytical Devices for Sub-Microliter Sample Analysis
title High-Resolution Microfluidic Paper-Based Analytical Devices for Sub-Microliter Sample Analysis
title_full High-Resolution Microfluidic Paper-Based Analytical Devices for Sub-Microliter Sample Analysis
title_fullStr High-Resolution Microfluidic Paper-Based Analytical Devices for Sub-Microliter Sample Analysis
title_full_unstemmed High-Resolution Microfluidic Paper-Based Analytical Devices for Sub-Microliter Sample Analysis
title_short High-Resolution Microfluidic Paper-Based Analytical Devices for Sub-Microliter Sample Analysis
title_sort high-resolution microfluidic paper-based analytical devices for sub-microliter sample analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189915/
https://www.ncbi.nlm.nih.gov/pubmed/30404255
http://dx.doi.org/10.3390/mi7050080
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