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Cytostretch, an Organ-on-Chip Platform

Organ-on-Chips (OOCs) are micro-fabricated devices which are used to culture cells in order to mimic functional units of human organs. The devices are designed to simulate the physiological environment of tissues in vivo. Cells in some types of OOCs can be stimulated in situ by electrical and/or mec...

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Autores principales: Gaio, Nikolas, van Meer, Berend, Quirós Solano, William, Bergers, Lambert, van de Stolpe, Anja, Mummery, Christine, Sarro, Pasqualina M., Dekker, Ronald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189941/
https://www.ncbi.nlm.nih.gov/pubmed/30404293
http://dx.doi.org/10.3390/mi7070120
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author Gaio, Nikolas
van Meer, Berend
Quirós Solano, William
Bergers, Lambert
van de Stolpe, Anja
Mummery, Christine
Sarro, Pasqualina M.
Dekker, Ronald
author_facet Gaio, Nikolas
van Meer, Berend
Quirós Solano, William
Bergers, Lambert
van de Stolpe, Anja
Mummery, Christine
Sarro, Pasqualina M.
Dekker, Ronald
author_sort Gaio, Nikolas
collection PubMed
description Organ-on-Chips (OOCs) are micro-fabricated devices which are used to culture cells in order to mimic functional units of human organs. The devices are designed to simulate the physiological environment of tissues in vivo. Cells in some types of OOCs can be stimulated in situ by electrical and/or mechanical actuators. These actuations can mimic physiological conditions in real tissue and may include fluid or air flow, or cyclic stretch and strain as they occur in the lung and heart. These conditions similarly affect cultured cells and may influence their ability to respond appropriately to physiological or pathological stimuli. To date, most focus has been on devices specifically designed to culture just one functional unit of a specific organ: lung alveoli, kidney nephrons or blood vessels, for example. In contrast, the modular Cytostretch membrane platform described here allows OOCs to be customized to different OOC applications. The platform utilizes silicon-based micro-fabrication techniques that allow low-cost, high-volume manufacturing. We describe the platform concept and its modules developed to date. Membrane variants include membranes with (i) through-membrane pores that allow biological signaling molecules to pass between two different tissue compartments; (ii) a stretchable micro-electrode array for electrical monitoring and stimulation; (iii) micro-patterning to promote cell alignment; and (iv) strain gauges to measure changes in substrate stress. This paper presents the fabrication and the proof of functionality for each module of the Cytostretch membrane. The assessment of each additional module demonstrate that a wide range of OOCs can be achieved.
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spelling pubmed-61899412018-11-01 Cytostretch, an Organ-on-Chip Platform Gaio, Nikolas van Meer, Berend Quirós Solano, William Bergers, Lambert van de Stolpe, Anja Mummery, Christine Sarro, Pasqualina M. Dekker, Ronald Micromachines (Basel) Article Organ-on-Chips (OOCs) are micro-fabricated devices which are used to culture cells in order to mimic functional units of human organs. The devices are designed to simulate the physiological environment of tissues in vivo. Cells in some types of OOCs can be stimulated in situ by electrical and/or mechanical actuators. These actuations can mimic physiological conditions in real tissue and may include fluid or air flow, or cyclic stretch and strain as they occur in the lung and heart. These conditions similarly affect cultured cells and may influence their ability to respond appropriately to physiological or pathological stimuli. To date, most focus has been on devices specifically designed to culture just one functional unit of a specific organ: lung alveoli, kidney nephrons or blood vessels, for example. In contrast, the modular Cytostretch membrane platform described here allows OOCs to be customized to different OOC applications. The platform utilizes silicon-based micro-fabrication techniques that allow low-cost, high-volume manufacturing. We describe the platform concept and its modules developed to date. Membrane variants include membranes with (i) through-membrane pores that allow biological signaling molecules to pass between two different tissue compartments; (ii) a stretchable micro-electrode array for electrical monitoring and stimulation; (iii) micro-patterning to promote cell alignment; and (iv) strain gauges to measure changes in substrate stress. This paper presents the fabrication and the proof of functionality for each module of the Cytostretch membrane. The assessment of each additional module demonstrate that a wide range of OOCs can be achieved. MDPI 2016-07-14 /pmc/articles/PMC6189941/ /pubmed/30404293 http://dx.doi.org/10.3390/mi7070120 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gaio, Nikolas
van Meer, Berend
Quirós Solano, William
Bergers, Lambert
van de Stolpe, Anja
Mummery, Christine
Sarro, Pasqualina M.
Dekker, Ronald
Cytostretch, an Organ-on-Chip Platform
title Cytostretch, an Organ-on-Chip Platform
title_full Cytostretch, an Organ-on-Chip Platform
title_fullStr Cytostretch, an Organ-on-Chip Platform
title_full_unstemmed Cytostretch, an Organ-on-Chip Platform
title_short Cytostretch, an Organ-on-Chip Platform
title_sort cytostretch, an organ-on-chip platform
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189941/
https://www.ncbi.nlm.nih.gov/pubmed/30404293
http://dx.doi.org/10.3390/mi7070120
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