Cargando…

Electroceutical Approach for Impairing the Motility of Pathogenic Bacterium Using a Microfluidic Platform

Electrotaxis, or galvanotaxis, refers to the migration pattern of cells induced in response to electrical potential. Electrotaxis has not been explored in detail in bacterial cells; information regarding the impact of current on pathogenic bacteria is severely lacking. Using microfluidic platforms a...

Descripción completa

Detalles Bibliográficos
Autores principales: Berthelot, Ryan, Doxsee, Kristina, Neethirajan, Suresh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189992/
https://www.ncbi.nlm.nih.gov/pubmed/30400398
http://dx.doi.org/10.3390/mi8070207
_version_ 1783363474242404352
author Berthelot, Ryan
Doxsee, Kristina
Neethirajan, Suresh
author_facet Berthelot, Ryan
Doxsee, Kristina
Neethirajan, Suresh
author_sort Berthelot, Ryan
collection PubMed
description Electrotaxis, or galvanotaxis, refers to the migration pattern of cells induced in response to electrical potential. Electrotaxis has not been explored in detail in bacterial cells; information regarding the impact of current on pathogenic bacteria is severely lacking. Using microfluidic platforms and optical microscopy, we designed a series of single- and multi-cue experiments to assess the impact of varying electrical currents and acetic acid concentrations on bacterial motility dynamics in pathogenic multi-drug resistant (MDR) strains of Pseudomonas aeruginosa and Escherichia coli. The use of the microfluidic platform allows for single-cue experiments where electrical current is supplied at a range that is biocidal to bacteria and multi-cue experiments where acetic acid is combined with current to enhance disinfection. These strategies may offer substantial therapeutic benefits, specifically for the treatment of biofilm infections, such as those found in the wound environment. Our results showed that an application of current in combination with acetic acid has profound inhibitory effects on MDR strains of P. aeruginosa and E. coli, even with brief applications. Specifically, E. coli motility dynamics and cell survival were significantly impaired starting at a concentration of 0.125 mA of direct current (DC) and 0.31% acetic acid, while P. aeruginosa was impaired at 0.70 mA and 0.31% acetic acid. As these strains are relevant wound pathogens, it is likely that this strategy would be effective against similar strains in vivo and could represent a new approach to hasten wound healing.
format Online
Article
Text
id pubmed-6189992
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-61899922018-11-01 Electroceutical Approach for Impairing the Motility of Pathogenic Bacterium Using a Microfluidic Platform Berthelot, Ryan Doxsee, Kristina Neethirajan, Suresh Micromachines (Basel) Article Electrotaxis, or galvanotaxis, refers to the migration pattern of cells induced in response to electrical potential. Electrotaxis has not been explored in detail in bacterial cells; information regarding the impact of current on pathogenic bacteria is severely lacking. Using microfluidic platforms and optical microscopy, we designed a series of single- and multi-cue experiments to assess the impact of varying electrical currents and acetic acid concentrations on bacterial motility dynamics in pathogenic multi-drug resistant (MDR) strains of Pseudomonas aeruginosa and Escherichia coli. The use of the microfluidic platform allows for single-cue experiments where electrical current is supplied at a range that is biocidal to bacteria and multi-cue experiments where acetic acid is combined with current to enhance disinfection. These strategies may offer substantial therapeutic benefits, specifically for the treatment of biofilm infections, such as those found in the wound environment. Our results showed that an application of current in combination with acetic acid has profound inhibitory effects on MDR strains of P. aeruginosa and E. coli, even with brief applications. Specifically, E. coli motility dynamics and cell survival were significantly impaired starting at a concentration of 0.125 mA of direct current (DC) and 0.31% acetic acid, while P. aeruginosa was impaired at 0.70 mA and 0.31% acetic acid. As these strains are relevant wound pathogens, it is likely that this strategy would be effective against similar strains in vivo and could represent a new approach to hasten wound healing. MDPI 2017-06-29 /pmc/articles/PMC6189992/ /pubmed/30400398 http://dx.doi.org/10.3390/mi8070207 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Berthelot, Ryan
Doxsee, Kristina
Neethirajan, Suresh
Electroceutical Approach for Impairing the Motility of Pathogenic Bacterium Using a Microfluidic Platform
title Electroceutical Approach for Impairing the Motility of Pathogenic Bacterium Using a Microfluidic Platform
title_full Electroceutical Approach for Impairing the Motility of Pathogenic Bacterium Using a Microfluidic Platform
title_fullStr Electroceutical Approach for Impairing the Motility of Pathogenic Bacterium Using a Microfluidic Platform
title_full_unstemmed Electroceutical Approach for Impairing the Motility of Pathogenic Bacterium Using a Microfluidic Platform
title_short Electroceutical Approach for Impairing the Motility of Pathogenic Bacterium Using a Microfluidic Platform
title_sort electroceutical approach for impairing the motility of pathogenic bacterium using a microfluidic platform
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189992/
https://www.ncbi.nlm.nih.gov/pubmed/30400398
http://dx.doi.org/10.3390/mi8070207
work_keys_str_mv AT berthelotryan electroceuticalapproachforimpairingthemotilityofpathogenicbacteriumusingamicrofluidicplatform
AT doxseekristina electroceuticalapproachforimpairingthemotilityofpathogenicbacteriumusingamicrofluidicplatform
AT neethirajansuresh electroceuticalapproachforimpairingthemotilityofpathogenicbacteriumusingamicrofluidicplatform