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Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring

Laboratory-based testing does not allow for the sufficiently rapid return of data to enable optimal therapeutic monitoring of patients with metabolic diseases such as phenylketonuria (PKU). The typical turn-around time of several days for current laboratory-based testing is too slow to be practicall...

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Detalles Bibliográficos
Autores principales: Robinson, Robert, Wong, Liam, Monnat, Raymond J., Fu, Elain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190008/
https://www.ncbi.nlm.nih.gov/pubmed/30407401
http://dx.doi.org/10.3390/mi7020028
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author Robinson, Robert
Wong, Liam
Monnat, Raymond J.
Fu, Elain
author_facet Robinson, Robert
Wong, Liam
Monnat, Raymond J.
Fu, Elain
author_sort Robinson, Robert
collection PubMed
description Laboratory-based testing does not allow for the sufficiently rapid return of data to enable optimal therapeutic monitoring of patients with metabolic diseases such as phenylketonuria (PKU). The typical turn-around time of several days for current laboratory-based testing is too slow to be practically useful for effective monitoring or optimizing therapy. This report describes the development of a rapid, paper-based, point-of-care device for phenylalanine detection using a small volume (40 μL) of whole blood. The quantitative resolution and reproducibility of this device with instrumented readout are described, together with the potential use of this device for point-of-care monitoring by PKU patients.
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spelling pubmed-61900082018-11-01 Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring Robinson, Robert Wong, Liam Monnat, Raymond J. Fu, Elain Micromachines (Basel) Article Laboratory-based testing does not allow for the sufficiently rapid return of data to enable optimal therapeutic monitoring of patients with metabolic diseases such as phenylketonuria (PKU). The typical turn-around time of several days for current laboratory-based testing is too slow to be practically useful for effective monitoring or optimizing therapy. This report describes the development of a rapid, paper-based, point-of-care device for phenylalanine detection using a small volume (40 μL) of whole blood. The quantitative resolution and reproducibility of this device with instrumented readout are described, together with the potential use of this device for point-of-care monitoring by PKU patients. MDPI 2016-02-15 /pmc/articles/PMC6190008/ /pubmed/30407401 http://dx.doi.org/10.3390/mi7020028 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Robinson, Robert
Wong, Liam
Monnat, Raymond J.
Fu, Elain
Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring
title Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring
title_full Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring
title_fullStr Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring
title_full_unstemmed Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring
title_short Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring
title_sort development of a whole blood paper-based device for phenylalanine detection in the context of pku therapy monitoring
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190008/
https://www.ncbi.nlm.nih.gov/pubmed/30407401
http://dx.doi.org/10.3390/mi7020028
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