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Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring
Laboratory-based testing does not allow for the sufficiently rapid return of data to enable optimal therapeutic monitoring of patients with metabolic diseases such as phenylketonuria (PKU). The typical turn-around time of several days for current laboratory-based testing is too slow to be practicall...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190008/ https://www.ncbi.nlm.nih.gov/pubmed/30407401 http://dx.doi.org/10.3390/mi7020028 |
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author | Robinson, Robert Wong, Liam Monnat, Raymond J. Fu, Elain |
author_facet | Robinson, Robert Wong, Liam Monnat, Raymond J. Fu, Elain |
author_sort | Robinson, Robert |
collection | PubMed |
description | Laboratory-based testing does not allow for the sufficiently rapid return of data to enable optimal therapeutic monitoring of patients with metabolic diseases such as phenylketonuria (PKU). The typical turn-around time of several days for current laboratory-based testing is too slow to be practically useful for effective monitoring or optimizing therapy. This report describes the development of a rapid, paper-based, point-of-care device for phenylalanine detection using a small volume (40 μL) of whole blood. The quantitative resolution and reproducibility of this device with instrumented readout are described, together with the potential use of this device for point-of-care monitoring by PKU patients. |
format | Online Article Text |
id | pubmed-6190008 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61900082018-11-01 Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring Robinson, Robert Wong, Liam Monnat, Raymond J. Fu, Elain Micromachines (Basel) Article Laboratory-based testing does not allow for the sufficiently rapid return of data to enable optimal therapeutic monitoring of patients with metabolic diseases such as phenylketonuria (PKU). The typical turn-around time of several days for current laboratory-based testing is too slow to be practically useful for effective monitoring or optimizing therapy. This report describes the development of a rapid, paper-based, point-of-care device for phenylalanine detection using a small volume (40 μL) of whole blood. The quantitative resolution and reproducibility of this device with instrumented readout are described, together with the potential use of this device for point-of-care monitoring by PKU patients. MDPI 2016-02-15 /pmc/articles/PMC6190008/ /pubmed/30407401 http://dx.doi.org/10.3390/mi7020028 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Robinson, Robert Wong, Liam Monnat, Raymond J. Fu, Elain Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring |
title | Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring |
title_full | Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring |
title_fullStr | Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring |
title_full_unstemmed | Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring |
title_short | Development of a Whole Blood Paper-Based Device for Phenylalanine Detection in the Context of PKU Therapy Monitoring |
title_sort | development of a whole blood paper-based device for phenylalanine detection in the context of pku therapy monitoring |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190008/ https://www.ncbi.nlm.nih.gov/pubmed/30407401 http://dx.doi.org/10.3390/mi7020028 |
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