Fabrication of a Cell Fixation Device for Robotic Cell Microinjection

Automation of cell microinjection greatly reduces operational difficulty, but cell fixation remains a challenge. Here, we describe an innovative device that solves the fixation problem without single-cell operation. The microarray cylinder is designed with a polydimethylsiloxane (PDMS) material surf...

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Detalles Bibliográficos
Autores principales: Xie, Yu, Zhou, Yunlei, Xi, Wenming, Zeng, Feng, Chen, Songyue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190162/
https://www.ncbi.nlm.nih.gov/pubmed/30404304
http://dx.doi.org/10.3390/mi7080131
Descripción
Sumario:Automation of cell microinjection greatly reduces operational difficulty, but cell fixation remains a challenge. Here, we describe an innovative device that solves the fixation problem without single-cell operation. The microarray cylinder is designed with a polydimethylsiloxane (PDMS) material surface to control the contact force between cells and the material. Data show that when the injection velocity exceeds 1.5 mm/s, microinjection success rate is over 80%. The maximum value of the adhesion force between the PDMS plate and the cell is 0.0138 N, and the need can be met in practical use of the robotic microinjection.

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