Fabrication of a Cell Fixation Device for Robotic Cell Microinjection

Automation of cell microinjection greatly reduces operational difficulty, but cell fixation remains a challenge. Here, we describe an innovative device that solves the fixation problem without single-cell operation. The microarray cylinder is designed with a polydimethylsiloxane (PDMS) material surf...

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Detalles Bibliográficos
Autores principales: Xie, Yu, Zhou, Yunlei, Xi, Wenming, Zeng, Feng, Chen, Songyue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190162/
https://www.ncbi.nlm.nih.gov/pubmed/30404304
http://dx.doi.org/10.3390/mi7080131
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author Xie, Yu
Zhou, Yunlei
Xi, Wenming
Zeng, Feng
Chen, Songyue
author_facet Xie, Yu
Zhou, Yunlei
Xi, Wenming
Zeng, Feng
Chen, Songyue
author_sort Xie, Yu
collection PubMed
description Automation of cell microinjection greatly reduces operational difficulty, but cell fixation remains a challenge. Here, we describe an innovative device that solves the fixation problem without single-cell operation. The microarray cylinder is designed with a polydimethylsiloxane (PDMS) material surface to control the contact force between cells and the material. Data show that when the injection velocity exceeds 1.5 mm/s, microinjection success rate is over 80%. The maximum value of the adhesion force between the PDMS plate and the cell is 0.0138 N, and the need can be met in practical use of the robotic microinjection.
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spelling pubmed-61901622018-11-01 Fabrication of a Cell Fixation Device for Robotic Cell Microinjection Xie, Yu Zhou, Yunlei Xi, Wenming Zeng, Feng Chen, Songyue Micromachines (Basel) Letter Automation of cell microinjection greatly reduces operational difficulty, but cell fixation remains a challenge. Here, we describe an innovative device that solves the fixation problem without single-cell operation. The microarray cylinder is designed with a polydimethylsiloxane (PDMS) material surface to control the contact force between cells and the material. Data show that when the injection velocity exceeds 1.5 mm/s, microinjection success rate is over 80%. The maximum value of the adhesion force between the PDMS plate and the cell is 0.0138 N, and the need can be met in practical use of the robotic microinjection. MDPI 2016-08-04 /pmc/articles/PMC6190162/ /pubmed/30404304 http://dx.doi.org/10.3390/mi7080131 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Letter
Xie, Yu
Zhou, Yunlei
Xi, Wenming
Zeng, Feng
Chen, Songyue
Fabrication of a Cell Fixation Device for Robotic Cell Microinjection
title Fabrication of a Cell Fixation Device for Robotic Cell Microinjection
title_full Fabrication of a Cell Fixation Device for Robotic Cell Microinjection
title_fullStr Fabrication of a Cell Fixation Device for Robotic Cell Microinjection
title_full_unstemmed Fabrication of a Cell Fixation Device for Robotic Cell Microinjection
title_short Fabrication of a Cell Fixation Device for Robotic Cell Microinjection
title_sort fabrication of a cell fixation device for robotic cell microinjection
topic Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190162/
https://www.ncbi.nlm.nih.gov/pubmed/30404304
http://dx.doi.org/10.3390/mi7080131
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AT zhouyunlei fabricationofacellfixationdeviceforroboticcellmicroinjection
AT xiwenming fabricationofacellfixationdeviceforroboticcellmicroinjection
AT zengfeng fabricationofacellfixationdeviceforroboticcellmicroinjection
AT chensongyue fabricationofacellfixationdeviceforroboticcellmicroinjection

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