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Simulation Analysis of Improving Microfluidic Heterogeneous Immunoassay Using Induced Charge Electroosmosis on a Floating Gate

On-chip immuno-sensors are a hot topic in the microfluidic community, which is usually limited by slow diffusion-dominated transport of analytes in confined microchannels. Specifically, the antigen-antibody binding reaction at a functionalized area cannot be provided with enough antigen source near...

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Detalles Bibliográficos
Autores principales: Hu, Qingming, Ren, Yukun, Liu, Weiyu, Tao, Ye, Jiang, Hongyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190211/
https://www.ncbi.nlm.nih.gov/pubmed/30400403
http://dx.doi.org/10.3390/mi8070212
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author Hu, Qingming
Ren, Yukun
Liu, Weiyu
Tao, Ye
Jiang, Hongyuan
author_facet Hu, Qingming
Ren, Yukun
Liu, Weiyu
Tao, Ye
Jiang, Hongyuan
author_sort Hu, Qingming
collection PubMed
description On-chip immuno-sensors are a hot topic in the microfluidic community, which is usually limited by slow diffusion-dominated transport of analytes in confined microchannels. Specifically, the antigen-antibody binding reaction at a functionalized area cannot be provided with enough antigen source near the reaction surface, since a small diffusion flux cannot match with the quick rate of surface reaction, which influences the response time and sensitivity of on-chip heterogeneous immunoassay. In this work, we propose a method to enhance the transportation of biomolecules to the surface of an antibody-immobilized electrode with induce charge electroosmotic (ICEO) convection in a low concentration suspension, so as to improve the binding efficiency of microfluidic heterogeneous immunoassays. The circular stirring fluid motion of ICEO on the surface of a floating gate electrode at the channel bottom accelerates the transport of freely suspended antigen towards the wall-immobilized antibodies. We investigate the dependence of binding efficiency on voltage magnitude and field frequency of the applied alternate current (AC) electrical field. The binding rate yields a factor of 5.4 higher binding for an applied voltage of 4 V at 10 Hz when the Damkohler number is 1000. The proposed microfluidic immuno-sensor technology of a simple electrode structure using ICEO convective fluid flow around floating conductors could offer exciting opportunities for diffusion-limited on-chip bio-microfluidic sensors.
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spelling pubmed-61902112018-11-01 Simulation Analysis of Improving Microfluidic Heterogeneous Immunoassay Using Induced Charge Electroosmosis on a Floating Gate Hu, Qingming Ren, Yukun Liu, Weiyu Tao, Ye Jiang, Hongyuan Micromachines (Basel) Article On-chip immuno-sensors are a hot topic in the microfluidic community, which is usually limited by slow diffusion-dominated transport of analytes in confined microchannels. Specifically, the antigen-antibody binding reaction at a functionalized area cannot be provided with enough antigen source near the reaction surface, since a small diffusion flux cannot match with the quick rate of surface reaction, which influences the response time and sensitivity of on-chip heterogeneous immunoassay. In this work, we propose a method to enhance the transportation of biomolecules to the surface of an antibody-immobilized electrode with induce charge electroosmotic (ICEO) convection in a low concentration suspension, so as to improve the binding efficiency of microfluidic heterogeneous immunoassays. The circular stirring fluid motion of ICEO on the surface of a floating gate electrode at the channel bottom accelerates the transport of freely suspended antigen towards the wall-immobilized antibodies. We investigate the dependence of binding efficiency on voltage magnitude and field frequency of the applied alternate current (AC) electrical field. The binding rate yields a factor of 5.4 higher binding for an applied voltage of 4 V at 10 Hz when the Damkohler number is 1000. The proposed microfluidic immuno-sensor technology of a simple electrode structure using ICEO convective fluid flow around floating conductors could offer exciting opportunities for diffusion-limited on-chip bio-microfluidic sensors. MDPI 2017-07-04 /pmc/articles/PMC6190211/ /pubmed/30400403 http://dx.doi.org/10.3390/mi8070212 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hu, Qingming
Ren, Yukun
Liu, Weiyu
Tao, Ye
Jiang, Hongyuan
Simulation Analysis of Improving Microfluidic Heterogeneous Immunoassay Using Induced Charge Electroosmosis on a Floating Gate
title Simulation Analysis of Improving Microfluidic Heterogeneous Immunoassay Using Induced Charge Electroosmosis on a Floating Gate
title_full Simulation Analysis of Improving Microfluidic Heterogeneous Immunoassay Using Induced Charge Electroosmosis on a Floating Gate
title_fullStr Simulation Analysis of Improving Microfluidic Heterogeneous Immunoassay Using Induced Charge Electroosmosis on a Floating Gate
title_full_unstemmed Simulation Analysis of Improving Microfluidic Heterogeneous Immunoassay Using Induced Charge Electroosmosis on a Floating Gate
title_short Simulation Analysis of Improving Microfluidic Heterogeneous Immunoassay Using Induced Charge Electroosmosis on a Floating Gate
title_sort simulation analysis of improving microfluidic heterogeneous immunoassay using induced charge electroosmosis on a floating gate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190211/
https://www.ncbi.nlm.nih.gov/pubmed/30400403
http://dx.doi.org/10.3390/mi8070212
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