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Numerical Investigation of a Novel Wiring Scheme Enabling Simple and Accurate Impedance Cytometry
Microfluidic impedance cytometry is a label-free approach for high-throughput analysis of particles and cells. It is based on the characterization of the dielectric properties of single particles as they flow through a microchannel with integrated electrodes. However, the measured signal depends not...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190262/ https://www.ncbi.nlm.nih.gov/pubmed/30400471 http://dx.doi.org/10.3390/mi8090283 |
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author | Caselli, Federica Reale, Riccardo Nodargi, Nicola Antonio Bisegna, Paolo |
author_facet | Caselli, Federica Reale, Riccardo Nodargi, Nicola Antonio Bisegna, Paolo |
author_sort | Caselli, Federica |
collection | PubMed |
description | Microfluidic impedance cytometry is a label-free approach for high-throughput analysis of particles and cells. It is based on the characterization of the dielectric properties of single particles as they flow through a microchannel with integrated electrodes. However, the measured signal depends not only on the intrinsic particle properties, but also on the particle trajectory through the measuring region, thus challenging the resolution and accuracy of the technique. In this work we show via simulation that this issue can be overcome without resorting to particle focusing, by means of a straightforward modification of the wiring scheme for the most typical and widely used microfluidic impedance chip. |
format | Online Article Text |
id | pubmed-6190262 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61902622018-11-01 Numerical Investigation of a Novel Wiring Scheme Enabling Simple and Accurate Impedance Cytometry Caselli, Federica Reale, Riccardo Nodargi, Nicola Antonio Bisegna, Paolo Micromachines (Basel) Article Microfluidic impedance cytometry is a label-free approach for high-throughput analysis of particles and cells. It is based on the characterization of the dielectric properties of single particles as they flow through a microchannel with integrated electrodes. However, the measured signal depends not only on the intrinsic particle properties, but also on the particle trajectory through the measuring region, thus challenging the resolution and accuracy of the technique. In this work we show via simulation that this issue can be overcome without resorting to particle focusing, by means of a straightforward modification of the wiring scheme for the most typical and widely used microfluidic impedance chip. MDPI 2017-09-18 /pmc/articles/PMC6190262/ /pubmed/30400471 http://dx.doi.org/10.3390/mi8090283 Text en © 2017 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Caselli, Federica Reale, Riccardo Nodargi, Nicola Antonio Bisegna, Paolo Numerical Investigation of a Novel Wiring Scheme Enabling Simple and Accurate Impedance Cytometry |
title | Numerical Investigation of a Novel Wiring Scheme Enabling Simple and Accurate Impedance Cytometry |
title_full | Numerical Investigation of a Novel Wiring Scheme Enabling Simple and Accurate Impedance Cytometry |
title_fullStr | Numerical Investigation of a Novel Wiring Scheme Enabling Simple and Accurate Impedance Cytometry |
title_full_unstemmed | Numerical Investigation of a Novel Wiring Scheme Enabling Simple and Accurate Impedance Cytometry |
title_short | Numerical Investigation of a Novel Wiring Scheme Enabling Simple and Accurate Impedance Cytometry |
title_sort | numerical investigation of a novel wiring scheme enabling simple and accurate impedance cytometry |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190262/ https://www.ncbi.nlm.nih.gov/pubmed/30400471 http://dx.doi.org/10.3390/mi8090283 |
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