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Numerical Investigation of a Novel Wiring Scheme Enabling Simple and Accurate Impedance Cytometry

Microfluidic impedance cytometry is a label-free approach for high-throughput analysis of particles and cells. It is based on the characterization of the dielectric properties of single particles as they flow through a microchannel with integrated electrodes. However, the measured signal depends not...

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Autores principales: Caselli, Federica, Reale, Riccardo, Nodargi, Nicola Antonio, Bisegna, Paolo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190262/
https://www.ncbi.nlm.nih.gov/pubmed/30400471
http://dx.doi.org/10.3390/mi8090283
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author Caselli, Federica
Reale, Riccardo
Nodargi, Nicola Antonio
Bisegna, Paolo
author_facet Caselli, Federica
Reale, Riccardo
Nodargi, Nicola Antonio
Bisegna, Paolo
author_sort Caselli, Federica
collection PubMed
description Microfluidic impedance cytometry is a label-free approach for high-throughput analysis of particles and cells. It is based on the characterization of the dielectric properties of single particles as they flow through a microchannel with integrated electrodes. However, the measured signal depends not only on the intrinsic particle properties, but also on the particle trajectory through the measuring region, thus challenging the resolution and accuracy of the technique. In this work we show via simulation that this issue can be overcome without resorting to particle focusing, by means of a straightforward modification of the wiring scheme for the most typical and widely used microfluidic impedance chip.
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spelling pubmed-61902622018-11-01 Numerical Investigation of a Novel Wiring Scheme Enabling Simple and Accurate Impedance Cytometry Caselli, Federica Reale, Riccardo Nodargi, Nicola Antonio Bisegna, Paolo Micromachines (Basel) Article Microfluidic impedance cytometry is a label-free approach for high-throughput analysis of particles and cells. It is based on the characterization of the dielectric properties of single particles as they flow through a microchannel with integrated electrodes. However, the measured signal depends not only on the intrinsic particle properties, but also on the particle trajectory through the measuring region, thus challenging the resolution and accuracy of the technique. In this work we show via simulation that this issue can be overcome without resorting to particle focusing, by means of a straightforward modification of the wiring scheme for the most typical and widely used microfluidic impedance chip. MDPI 2017-09-18 /pmc/articles/PMC6190262/ /pubmed/30400471 http://dx.doi.org/10.3390/mi8090283 Text en © 2017 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Caselli, Federica
Reale, Riccardo
Nodargi, Nicola Antonio
Bisegna, Paolo
Numerical Investigation of a Novel Wiring Scheme Enabling Simple and Accurate Impedance Cytometry
title Numerical Investigation of a Novel Wiring Scheme Enabling Simple and Accurate Impedance Cytometry
title_full Numerical Investigation of a Novel Wiring Scheme Enabling Simple and Accurate Impedance Cytometry
title_fullStr Numerical Investigation of a Novel Wiring Scheme Enabling Simple and Accurate Impedance Cytometry
title_full_unstemmed Numerical Investigation of a Novel Wiring Scheme Enabling Simple and Accurate Impedance Cytometry
title_short Numerical Investigation of a Novel Wiring Scheme Enabling Simple and Accurate Impedance Cytometry
title_sort numerical investigation of a novel wiring scheme enabling simple and accurate impedance cytometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190262/
https://www.ncbi.nlm.nih.gov/pubmed/30400471
http://dx.doi.org/10.3390/mi8090283
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