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Rapid Fabrication of Electrophoretic Microfluidic Devices from Polyester, Adhesives and Gold Leaf

In the last decade, the microfluidic community has witnessed an evolution in fabrication methodologies that deviate from using conventional glass and polymer-based materials. A leading example within this group is the print, cut and laminate (PCL) approach, which entails the laser cutting of microfl...

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Autores principales: Birch, Christopher, DuVall, Jacquelyn A., Le Roux, Delphine, Thompson, Brandon L., Tsuei, An-Chi, Li, Jingyi, Nelson, Daniel A., Mills, Daniel L., Landers, James P., Root, Brian E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190314/
http://dx.doi.org/10.3390/mi8010017
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author Birch, Christopher
DuVall, Jacquelyn A.
Le Roux, Delphine
Thompson, Brandon L.
Tsuei, An-Chi
Li, Jingyi
Nelson, Daniel A.
Mills, Daniel L.
Landers, James P.
Root, Brian E.
author_facet Birch, Christopher
DuVall, Jacquelyn A.
Le Roux, Delphine
Thompson, Brandon L.
Tsuei, An-Chi
Li, Jingyi
Nelson, Daniel A.
Mills, Daniel L.
Landers, James P.
Root, Brian E.
author_sort Birch, Christopher
collection PubMed
description In the last decade, the microfluidic community has witnessed an evolution in fabrication methodologies that deviate from using conventional glass and polymer-based materials. A leading example within this group is the print, cut and laminate (PCL) approach, which entails the laser cutting of microfluidic architecture into ink toner-laden polyester sheets, followed by the lamination of these layers for device assembly. Recent success when applying this method to human genetic fingerprinting has highlighted that it is now ripe for the refinements necessary to render it amenable to mass-manufacture. In this communication, we detail those modifications by identifying and implementing a suitable heat-sensitive adhesive (HSA) material to equip the devices with the durability and resilience required for commercialization and fieldwork. Importantly, this augmentation is achieved without sacrificing any of the characteristics which make the PCL approach attractive for prototyping. Exemplary HSA-devices performed DNA extraction, amplification and separation which, when combined, constitute the complete sequence necessary for human profiling and other DNA-based analyses.
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spelling pubmed-61903142018-11-01 Rapid Fabrication of Electrophoretic Microfluidic Devices from Polyester, Adhesives and Gold Leaf Birch, Christopher DuVall, Jacquelyn A. Le Roux, Delphine Thompson, Brandon L. Tsuei, An-Chi Li, Jingyi Nelson, Daniel A. Mills, Daniel L. Landers, James P. Root, Brian E. Micromachines (Basel) Communication In the last decade, the microfluidic community has witnessed an evolution in fabrication methodologies that deviate from using conventional glass and polymer-based materials. A leading example within this group is the print, cut and laminate (PCL) approach, which entails the laser cutting of microfluidic architecture into ink toner-laden polyester sheets, followed by the lamination of these layers for device assembly. Recent success when applying this method to human genetic fingerprinting has highlighted that it is now ripe for the refinements necessary to render it amenable to mass-manufacture. In this communication, we detail those modifications by identifying and implementing a suitable heat-sensitive adhesive (HSA) material to equip the devices with the durability and resilience required for commercialization and fieldwork. Importantly, this augmentation is achieved without sacrificing any of the characteristics which make the PCL approach attractive for prototyping. Exemplary HSA-devices performed DNA extraction, amplification and separation which, when combined, constitute the complete sequence necessary for human profiling and other DNA-based analyses. MDPI 2017-01-09 /pmc/articles/PMC6190314/ http://dx.doi.org/10.3390/mi8010017 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Birch, Christopher
DuVall, Jacquelyn A.
Le Roux, Delphine
Thompson, Brandon L.
Tsuei, An-Chi
Li, Jingyi
Nelson, Daniel A.
Mills, Daniel L.
Landers, James P.
Root, Brian E.
Rapid Fabrication of Electrophoretic Microfluidic Devices from Polyester, Adhesives and Gold Leaf
title Rapid Fabrication of Electrophoretic Microfluidic Devices from Polyester, Adhesives and Gold Leaf
title_full Rapid Fabrication of Electrophoretic Microfluidic Devices from Polyester, Adhesives and Gold Leaf
title_fullStr Rapid Fabrication of Electrophoretic Microfluidic Devices from Polyester, Adhesives and Gold Leaf
title_full_unstemmed Rapid Fabrication of Electrophoretic Microfluidic Devices from Polyester, Adhesives and Gold Leaf
title_short Rapid Fabrication of Electrophoretic Microfluidic Devices from Polyester, Adhesives and Gold Leaf
title_sort rapid fabrication of electrophoretic microfluidic devices from polyester, adhesives and gold leaf
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190314/
http://dx.doi.org/10.3390/mi8010017
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