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High Throughput Studies of Cell Migration in 3D Microtissues Fabricated by a Droplet Microfluidic Chip

Arrayed three-dimensional (3D) micro-sized tissues with encapsulated cells (microtissues) have been fabricated by a droplet microfluidic chip. The extracellular matrix (ECM) is a polymerized collagen network. One or multiple breast cancer cells were embedded within the microtissues, which were store...

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Detalles Bibliográficos
Autores principales: Che, Xiangchen, Nuhn, Jacob, Schneider, Ian, Que, Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190366/
https://www.ncbi.nlm.nih.gov/pubmed/30404258
http://dx.doi.org/10.3390/mi7050084
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author Che, Xiangchen
Nuhn, Jacob
Schneider, Ian
Que, Long
author_facet Che, Xiangchen
Nuhn, Jacob
Schneider, Ian
Que, Long
author_sort Che, Xiangchen
collection PubMed
description Arrayed three-dimensional (3D) micro-sized tissues with encapsulated cells (microtissues) have been fabricated by a droplet microfluidic chip. The extracellular matrix (ECM) is a polymerized collagen network. One or multiple breast cancer cells were embedded within the microtissues, which were stored in arrayed microchambers on the same chip without ECM droplet shrinkage over 48 h. The migration trajectory of the cells was recorded by optical microscopy. The migration speed was calculated in the range of 3–6 µm/h. Interestingly, cells in devices filled with a continuous collagen network migrated faster than those where only droplets were arrayed in the chambers. This is likely due to differences in the length scales of the ECM network, as cells embedded in thin collagen slabs also migrate slower than those in thick collagen slabs. In addition to migration, this technical platform can be potentially used to study cancer cell-stromal cell interactions and ECM remodeling in 3D tumor-mimicking environments.
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spelling pubmed-61903662018-11-01 High Throughput Studies of Cell Migration in 3D Microtissues Fabricated by a Droplet Microfluidic Chip Che, Xiangchen Nuhn, Jacob Schneider, Ian Que, Long Micromachines (Basel) Communication Arrayed three-dimensional (3D) micro-sized tissues with encapsulated cells (microtissues) have been fabricated by a droplet microfluidic chip. The extracellular matrix (ECM) is a polymerized collagen network. One or multiple breast cancer cells were embedded within the microtissues, which were stored in arrayed microchambers on the same chip without ECM droplet shrinkage over 48 h. The migration trajectory of the cells was recorded by optical microscopy. The migration speed was calculated in the range of 3–6 µm/h. Interestingly, cells in devices filled with a continuous collagen network migrated faster than those where only droplets were arrayed in the chambers. This is likely due to differences in the length scales of the ECM network, as cells embedded in thin collagen slabs also migrate slower than those in thick collagen slabs. In addition to migration, this technical platform can be potentially used to study cancer cell-stromal cell interactions and ECM remodeling in 3D tumor-mimicking environments. MDPI 2016-05-05 /pmc/articles/PMC6190366/ /pubmed/30404258 http://dx.doi.org/10.3390/mi7050084 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Che, Xiangchen
Nuhn, Jacob
Schneider, Ian
Que, Long
High Throughput Studies of Cell Migration in 3D Microtissues Fabricated by a Droplet Microfluidic Chip
title High Throughput Studies of Cell Migration in 3D Microtissues Fabricated by a Droplet Microfluidic Chip
title_full High Throughput Studies of Cell Migration in 3D Microtissues Fabricated by a Droplet Microfluidic Chip
title_fullStr High Throughput Studies of Cell Migration in 3D Microtissues Fabricated by a Droplet Microfluidic Chip
title_full_unstemmed High Throughput Studies of Cell Migration in 3D Microtissues Fabricated by a Droplet Microfluidic Chip
title_short High Throughput Studies of Cell Migration in 3D Microtissues Fabricated by a Droplet Microfluidic Chip
title_sort high throughput studies of cell migration in 3d microtissues fabricated by a droplet microfluidic chip
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190366/
https://www.ncbi.nlm.nih.gov/pubmed/30404258
http://dx.doi.org/10.3390/mi7050084
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