Cargando…

An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design

Although most in vitro (cell-free) synthetic biology projects are usually used for the purposes of fundamental research or the formation of high-value products, in vitro synthetic biology platform, which can implement complicated biochemical reactions by the in vitro assembly of numerous enzymes and...

Descripción completa

Detalles Bibliográficos
Autores principales: Shi, Ting, Han, Pingping, You, Chun, Zhang, Yi-Heng P. Job
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190512/
https://www.ncbi.nlm.nih.gov/pubmed/30345404
http://dx.doi.org/10.1016/j.synbio.2018.05.002
_version_ 1783363588803526656
author Shi, Ting
Han, Pingping
You, Chun
Zhang, Yi-Heng P. Job
author_facet Shi, Ting
Han, Pingping
You, Chun
Zhang, Yi-Heng P. Job
author_sort Shi, Ting
collection PubMed
description Although most in vitro (cell-free) synthetic biology projects are usually used for the purposes of fundamental research or the formation of high-value products, in vitro synthetic biology platform, which can implement complicated biochemical reactions by the in vitro assembly of numerous enzymes and coenzymes, has been proposed for low-cost biomanufacturing of bioenergy, food, biochemicals, and nutraceuticals. In addition to the most important advantage-high product yield, in vitro synthetic biology platform features several other biomanufacturing advantages, such as fast reaction rate, easy product separation, open process control, broad reaction condition, tolerance to toxic substrates or products, and so on. In this article, we present the basic bottom-up design principles of in vitro synthetic pathway from basic building blocks-BioBricks (thermoenzymes and/or immobilized enzymes) to building modules (e.g., enzyme complexes or multiple enzymes as a module) with specific functions. With development in thermostable building blocks-BioBricks and modules, the in vitro synthetic biology platform would open a new biomanufacturing age for the cost-competitive production of biocommodities.
format Online
Article
Text
id pubmed-6190512
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher KeAi Publishing
record_format MEDLINE/PubMed
spelling pubmed-61905122018-10-19 An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design Shi, Ting Han, Pingping You, Chun Zhang, Yi-Heng P. Job Synth Syst Biotechnol Article Although most in vitro (cell-free) synthetic biology projects are usually used for the purposes of fundamental research or the formation of high-value products, in vitro synthetic biology platform, which can implement complicated biochemical reactions by the in vitro assembly of numerous enzymes and coenzymes, has been proposed for low-cost biomanufacturing of bioenergy, food, biochemicals, and nutraceuticals. In addition to the most important advantage-high product yield, in vitro synthetic biology platform features several other biomanufacturing advantages, such as fast reaction rate, easy product separation, open process control, broad reaction condition, tolerance to toxic substrates or products, and so on. In this article, we present the basic bottom-up design principles of in vitro synthetic pathway from basic building blocks-BioBricks (thermoenzymes and/or immobilized enzymes) to building modules (e.g., enzyme complexes or multiple enzymes as a module) with specific functions. With development in thermostable building blocks-BioBricks and modules, the in vitro synthetic biology platform would open a new biomanufacturing age for the cost-competitive production of biocommodities. KeAi Publishing 2018-05-30 /pmc/articles/PMC6190512/ /pubmed/30345404 http://dx.doi.org/10.1016/j.synbio.2018.05.002 Text en © 2018 Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Shi, Ting
Han, Pingping
You, Chun
Zhang, Yi-Heng P. Job
An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design
title An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design
title_full An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design
title_fullStr An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design
title_full_unstemmed An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design
title_short An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design
title_sort in vitro synthetic biology platform for emerging industrial biomanufacturing: bottom-up pathway design
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190512/
https://www.ncbi.nlm.nih.gov/pubmed/30345404
http://dx.doi.org/10.1016/j.synbio.2018.05.002
work_keys_str_mv AT shiting aninvitrosyntheticbiologyplatformforemergingindustrialbiomanufacturingbottomuppathwaydesign
AT hanpingping aninvitrosyntheticbiologyplatformforemergingindustrialbiomanufacturingbottomuppathwaydesign
AT youchun aninvitrosyntheticbiologyplatformforemergingindustrialbiomanufacturingbottomuppathwaydesign
AT zhangyihengpjob aninvitrosyntheticbiologyplatformforemergingindustrialbiomanufacturingbottomuppathwaydesign
AT shiting invitrosyntheticbiologyplatformforemergingindustrialbiomanufacturingbottomuppathwaydesign
AT hanpingping invitrosyntheticbiologyplatformforemergingindustrialbiomanufacturingbottomuppathwaydesign
AT youchun invitrosyntheticbiologyplatformforemergingindustrialbiomanufacturingbottomuppathwaydesign
AT zhangyihengpjob invitrosyntheticbiologyplatformforemergingindustrialbiomanufacturingbottomuppathwaydesign