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Carbonic anhydrase IX enhances tumor cell proliferation and tumor progression in osteosarcoma
PURPOSE: We investigated the effect of carbonic anhydrase IX (CA IX) inhibitor under hypoxia and normoxia in SaOS2 human osteosarcoma cell line. We also evaluated the expression of CA IX in 27 patients diagnosed with osteosarcoma. MATERIALS AND METHODS: CA IX expression in SaOS2 cells cultured under...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190629/ https://www.ncbi.nlm.nih.gov/pubmed/30349321 http://dx.doi.org/10.2147/OTT.S177605 |
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author | Okuno, Kazuma Matsubara, Takao Nakamura, Tomoki Iino, Takahiro Kakimoto, Takuya Asanuma, Kunihiro Matsumine, Akihiko Sudo, Akihiro |
author_facet | Okuno, Kazuma Matsubara, Takao Nakamura, Tomoki Iino, Takahiro Kakimoto, Takuya Asanuma, Kunihiro Matsumine, Akihiko Sudo, Akihiro |
author_sort | Okuno, Kazuma |
collection | PubMed |
description | PURPOSE: We investigated the effect of carbonic anhydrase IX (CA IX) inhibitor under hypoxia and normoxia in SaOS2 human osteosarcoma cell line. We also evaluated the expression of CA IX in 27 patients diagnosed with osteosarcoma. MATERIALS AND METHODS: CA IX expression in SaOS2 cells cultured under different oxygen tensions was analyzed by Western blotting. To evaluate the effect of CA IX inhibitor, MTS cell viability assay was performed after cells were treated with various concentrations of doxorubicin with or without a CA IX inhibitor. Finally, CA IX expression in patient-derived osteosarcoma samples was evaluated by immunohistochemistry. RESULTS: Treatment with CA IX inhibitor significantly suppressed cell proliferation and migration under hypoxic conditions. CA IX expression was observed in 81% of 27 patients. The 5-year survival rates in patients with high and low stain scores were 43.8% and 81.8%, respectively. CONCLUSION: CA IX inhibitors have the potential to suppress cell proliferation, migration, and chemoresistance. |
format | Online Article Text |
id | pubmed-6190629 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-61906292018-10-22 Carbonic anhydrase IX enhances tumor cell proliferation and tumor progression in osteosarcoma Okuno, Kazuma Matsubara, Takao Nakamura, Tomoki Iino, Takahiro Kakimoto, Takuya Asanuma, Kunihiro Matsumine, Akihiko Sudo, Akihiro Onco Targets Ther Original Research PURPOSE: We investigated the effect of carbonic anhydrase IX (CA IX) inhibitor under hypoxia and normoxia in SaOS2 human osteosarcoma cell line. We also evaluated the expression of CA IX in 27 patients diagnosed with osteosarcoma. MATERIALS AND METHODS: CA IX expression in SaOS2 cells cultured under different oxygen tensions was analyzed by Western blotting. To evaluate the effect of CA IX inhibitor, MTS cell viability assay was performed after cells were treated with various concentrations of doxorubicin with or without a CA IX inhibitor. Finally, CA IX expression in patient-derived osteosarcoma samples was evaluated by immunohistochemistry. RESULTS: Treatment with CA IX inhibitor significantly suppressed cell proliferation and migration under hypoxic conditions. CA IX expression was observed in 81% of 27 patients. The 5-year survival rates in patients with high and low stain scores were 43.8% and 81.8%, respectively. CONCLUSION: CA IX inhibitors have the potential to suppress cell proliferation, migration, and chemoresistance. Dove Medical Press 2018-10-12 /pmc/articles/PMC6190629/ /pubmed/30349321 http://dx.doi.org/10.2147/OTT.S177605 Text en © 2018 Okuno et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Okuno, Kazuma Matsubara, Takao Nakamura, Tomoki Iino, Takahiro Kakimoto, Takuya Asanuma, Kunihiro Matsumine, Akihiko Sudo, Akihiro Carbonic anhydrase IX enhances tumor cell proliferation and tumor progression in osteosarcoma |
title | Carbonic anhydrase IX enhances tumor cell proliferation and tumor progression in osteosarcoma |
title_full | Carbonic anhydrase IX enhances tumor cell proliferation and tumor progression in osteosarcoma |
title_fullStr | Carbonic anhydrase IX enhances tumor cell proliferation and tumor progression in osteosarcoma |
title_full_unstemmed | Carbonic anhydrase IX enhances tumor cell proliferation and tumor progression in osteosarcoma |
title_short | Carbonic anhydrase IX enhances tumor cell proliferation and tumor progression in osteosarcoma |
title_sort | carbonic anhydrase ix enhances tumor cell proliferation and tumor progression in osteosarcoma |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190629/ https://www.ncbi.nlm.nih.gov/pubmed/30349321 http://dx.doi.org/10.2147/OTT.S177605 |
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