Cargando…

Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria

Rod-shaped mycobacteria expand from their poles, yet d-amino acid probes label cell wall peptidoglycan in this genus at both the poles and sidewall. We sought to clarify the metabolic fates of these probes. Monopeptide incorporation was decreased by antibiotics that block peptidoglycan synthesis or...

Descripción completa

Detalles Bibliográficos
Autores principales: García-Heredia, Alam, Pohane, Amol Arunrao, Melzer, Emily S, Carr, Caleb R, Fiolek, Taylor J, Rundell, Sarah R, Lim, Hoong Chuin, Wagner, Jeffrey C, Morita, Yasu S, Swarts, Benjamin M, Siegrist, M Sloan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6191288/
https://www.ncbi.nlm.nih.gov/pubmed/30198841
http://dx.doi.org/10.7554/eLife.37243
_version_ 1783363699154616320
author García-Heredia, Alam
Pohane, Amol Arunrao
Melzer, Emily S
Carr, Caleb R
Fiolek, Taylor J
Rundell, Sarah R
Lim, Hoong Chuin
Wagner, Jeffrey C
Morita, Yasu S
Swarts, Benjamin M
Siegrist, M Sloan
author_facet García-Heredia, Alam
Pohane, Amol Arunrao
Melzer, Emily S
Carr, Caleb R
Fiolek, Taylor J
Rundell, Sarah R
Lim, Hoong Chuin
Wagner, Jeffrey C
Morita, Yasu S
Swarts, Benjamin M
Siegrist, M Sloan
author_sort García-Heredia, Alam
collection PubMed
description Rod-shaped mycobacteria expand from their poles, yet d-amino acid probes label cell wall peptidoglycan in this genus at both the poles and sidewall. We sought to clarify the metabolic fates of these probes. Monopeptide incorporation was decreased by antibiotics that block peptidoglycan synthesis or l,d-transpeptidation and in an l,d-transpeptidase mutant. Dipeptides complemented defects in d-alanine synthesis or ligation and were present in lipid-linked peptidoglycan precursors. Characterizing probe uptake pathways allowed us to localize peptidoglycan metabolism with precision: monopeptide-marked l,d-transpeptidase remodeling and dipeptide-marked synthesis were coincident with mycomembrane metabolism at the poles, septum and sidewall. Fluorescent pencillin-marked d,d-transpeptidation around the cell perimeter further suggested that the mycobacterial sidewall is a site of cell wall assembly. While polar peptidoglycan synthesis was associated with cell elongation, sidewall synthesis responded to cell wall damage. Peptidoglycan editing along the sidewall may support cell wall robustness in pole-growing mycobacteria.
format Online
Article
Text
id pubmed-6191288
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher eLife Sciences Publications, Ltd
record_format MEDLINE/PubMed
spelling pubmed-61912882018-10-21 Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria García-Heredia, Alam Pohane, Amol Arunrao Melzer, Emily S Carr, Caleb R Fiolek, Taylor J Rundell, Sarah R Lim, Hoong Chuin Wagner, Jeffrey C Morita, Yasu S Swarts, Benjamin M Siegrist, M Sloan eLife Biochemistry and Chemical Biology Rod-shaped mycobacteria expand from their poles, yet d-amino acid probes label cell wall peptidoglycan in this genus at both the poles and sidewall. We sought to clarify the metabolic fates of these probes. Monopeptide incorporation was decreased by antibiotics that block peptidoglycan synthesis or l,d-transpeptidation and in an l,d-transpeptidase mutant. Dipeptides complemented defects in d-alanine synthesis or ligation and were present in lipid-linked peptidoglycan precursors. Characterizing probe uptake pathways allowed us to localize peptidoglycan metabolism with precision: monopeptide-marked l,d-transpeptidase remodeling and dipeptide-marked synthesis were coincident with mycomembrane metabolism at the poles, septum and sidewall. Fluorescent pencillin-marked d,d-transpeptidation around the cell perimeter further suggested that the mycobacterial sidewall is a site of cell wall assembly. While polar peptidoglycan synthesis was associated with cell elongation, sidewall synthesis responded to cell wall damage. Peptidoglycan editing along the sidewall may support cell wall robustness in pole-growing mycobacteria. eLife Sciences Publications, Ltd 2018-09-10 /pmc/articles/PMC6191288/ /pubmed/30198841 http://dx.doi.org/10.7554/eLife.37243 Text en © 2018, García-Heredia et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biochemistry and Chemical Biology
García-Heredia, Alam
Pohane, Amol Arunrao
Melzer, Emily S
Carr, Caleb R
Fiolek, Taylor J
Rundell, Sarah R
Lim, Hoong Chuin
Wagner, Jeffrey C
Morita, Yasu S
Swarts, Benjamin M
Siegrist, M Sloan
Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria
title Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria
title_full Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria
title_fullStr Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria
title_full_unstemmed Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria
title_short Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria
title_sort peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria
topic Biochemistry and Chemical Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6191288/
https://www.ncbi.nlm.nih.gov/pubmed/30198841
http://dx.doi.org/10.7554/eLife.37243
work_keys_str_mv AT garciaherediaalam peptidoglycanprecursorsynthesisalongthesidewallofpolegrowingmycobacteria
AT pohaneamolarunrao peptidoglycanprecursorsynthesisalongthesidewallofpolegrowingmycobacteria
AT melzeremilys peptidoglycanprecursorsynthesisalongthesidewallofpolegrowingmycobacteria
AT carrcalebr peptidoglycanprecursorsynthesisalongthesidewallofpolegrowingmycobacteria
AT fiolektaylorj peptidoglycanprecursorsynthesisalongthesidewallofpolegrowingmycobacteria
AT rundellsarahr peptidoglycanprecursorsynthesisalongthesidewallofpolegrowingmycobacteria
AT limhoongchuin peptidoglycanprecursorsynthesisalongthesidewallofpolegrowingmycobacteria
AT wagnerjeffreyc peptidoglycanprecursorsynthesisalongthesidewallofpolegrowingmycobacteria
AT moritayasus peptidoglycanprecursorsynthesisalongthesidewallofpolegrowingmycobacteria
AT swartsbenjaminm peptidoglycanprecursorsynthesisalongthesidewallofpolegrowingmycobacteria
AT siegristmsloan peptidoglycanprecursorsynthesisalongthesidewallofpolegrowingmycobacteria