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Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria
Rod-shaped mycobacteria expand from their poles, yet d-amino acid probes label cell wall peptidoglycan in this genus at both the poles and sidewall. We sought to clarify the metabolic fates of these probes. Monopeptide incorporation was decreased by antibiotics that block peptidoglycan synthesis or...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6191288/ https://www.ncbi.nlm.nih.gov/pubmed/30198841 http://dx.doi.org/10.7554/eLife.37243 |
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author | García-Heredia, Alam Pohane, Amol Arunrao Melzer, Emily S Carr, Caleb R Fiolek, Taylor J Rundell, Sarah R Lim, Hoong Chuin Wagner, Jeffrey C Morita, Yasu S Swarts, Benjamin M Siegrist, M Sloan |
author_facet | García-Heredia, Alam Pohane, Amol Arunrao Melzer, Emily S Carr, Caleb R Fiolek, Taylor J Rundell, Sarah R Lim, Hoong Chuin Wagner, Jeffrey C Morita, Yasu S Swarts, Benjamin M Siegrist, M Sloan |
author_sort | García-Heredia, Alam |
collection | PubMed |
description | Rod-shaped mycobacteria expand from their poles, yet d-amino acid probes label cell wall peptidoglycan in this genus at both the poles and sidewall. We sought to clarify the metabolic fates of these probes. Monopeptide incorporation was decreased by antibiotics that block peptidoglycan synthesis or l,d-transpeptidation and in an l,d-transpeptidase mutant. Dipeptides complemented defects in d-alanine synthesis or ligation and were present in lipid-linked peptidoglycan precursors. Characterizing probe uptake pathways allowed us to localize peptidoglycan metabolism with precision: monopeptide-marked l,d-transpeptidase remodeling and dipeptide-marked synthesis were coincident with mycomembrane metabolism at the poles, septum and sidewall. Fluorescent pencillin-marked d,d-transpeptidation around the cell perimeter further suggested that the mycobacterial sidewall is a site of cell wall assembly. While polar peptidoglycan synthesis was associated with cell elongation, sidewall synthesis responded to cell wall damage. Peptidoglycan editing along the sidewall may support cell wall robustness in pole-growing mycobacteria. |
format | Online Article Text |
id | pubmed-6191288 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-61912882018-10-21 Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria García-Heredia, Alam Pohane, Amol Arunrao Melzer, Emily S Carr, Caleb R Fiolek, Taylor J Rundell, Sarah R Lim, Hoong Chuin Wagner, Jeffrey C Morita, Yasu S Swarts, Benjamin M Siegrist, M Sloan eLife Biochemistry and Chemical Biology Rod-shaped mycobacteria expand from their poles, yet d-amino acid probes label cell wall peptidoglycan in this genus at both the poles and sidewall. We sought to clarify the metabolic fates of these probes. Monopeptide incorporation was decreased by antibiotics that block peptidoglycan synthesis or l,d-transpeptidation and in an l,d-transpeptidase mutant. Dipeptides complemented defects in d-alanine synthesis or ligation and were present in lipid-linked peptidoglycan precursors. Characterizing probe uptake pathways allowed us to localize peptidoglycan metabolism with precision: monopeptide-marked l,d-transpeptidase remodeling and dipeptide-marked synthesis were coincident with mycomembrane metabolism at the poles, septum and sidewall. Fluorescent pencillin-marked d,d-transpeptidation around the cell perimeter further suggested that the mycobacterial sidewall is a site of cell wall assembly. While polar peptidoglycan synthesis was associated with cell elongation, sidewall synthesis responded to cell wall damage. Peptidoglycan editing along the sidewall may support cell wall robustness in pole-growing mycobacteria. eLife Sciences Publications, Ltd 2018-09-10 /pmc/articles/PMC6191288/ /pubmed/30198841 http://dx.doi.org/10.7554/eLife.37243 Text en © 2018, García-Heredia et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Biochemistry and Chemical Biology García-Heredia, Alam Pohane, Amol Arunrao Melzer, Emily S Carr, Caleb R Fiolek, Taylor J Rundell, Sarah R Lim, Hoong Chuin Wagner, Jeffrey C Morita, Yasu S Swarts, Benjamin M Siegrist, M Sloan Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria |
title | Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria |
title_full | Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria |
title_fullStr | Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria |
title_full_unstemmed | Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria |
title_short | Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria |
title_sort | peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria |
topic | Biochemistry and Chemical Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6191288/ https://www.ncbi.nlm.nih.gov/pubmed/30198841 http://dx.doi.org/10.7554/eLife.37243 |
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