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Effects of Cotton Seed Powder as the Seed Medium Nitrogen Source on the Morphology and Pneumocandin B(0) Yield of Glarea lozoyensis
Pneumocandin B(0) is an important antifungal drug precursor produced by filamentous fungus Glarea lozoyensis. The high broth viscosity of cultures of this organism results in lower oxygen solubility and higher energy consumption for agitation and aeration, which mostly caused by the morphologies of...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6191469/ https://www.ncbi.nlm.nih.gov/pubmed/30364147 http://dx.doi.org/10.3389/fmicb.2018.02352 |
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author | Song, Ping Yuan, Kai Ji, Xiao-Jun Ren, Lu-Jing Zhang, Sen Wen, Jian-Ping Huang, He |
author_facet | Song, Ping Yuan, Kai Ji, Xiao-Jun Ren, Lu-Jing Zhang, Sen Wen, Jian-Ping Huang, He |
author_sort | Song, Ping |
collection | PubMed |
description | Pneumocandin B(0) is an important antifungal drug precursor produced by filamentous fungus Glarea lozoyensis. The high broth viscosity of cultures of this organism results in lower oxygen solubility and higher energy consumption for agitation and aeration, which mostly caused by the morphologies of filamentous fungi in submerged culture. In this study, the effects of different seed medium nitrogen sources on morphology and fermentation behavior of G. lozoyensis were investigated, and cotton seed powder resulted in small, compact pellets. Moreover, pneumocandin B(0) yield in Erlenmeyer flasks were increased by 22.9%. Furthermore, pneumocandin B(0) yield in a 50-L fermenter reached 2,100 mg/L and the dissolved oxygen maintained above 30%. Additionally, activities of phosphofructokinase (PFK), isocitrate dehydrogenase (ICDH), glucose 6-phosphate dehydrogenase (G6PDH), and malic enzyme (ME) were increased by 87.5, 50, 41.6, and 10.7%, respectively. This study demonstrates the feasibility and advantages of using cotton seed powder for controlling the fungal morphology and improving the product yield in pneumocandin fermentations. |
format | Online Article Text |
id | pubmed-6191469 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-61914692018-10-24 Effects of Cotton Seed Powder as the Seed Medium Nitrogen Source on the Morphology and Pneumocandin B(0) Yield of Glarea lozoyensis Song, Ping Yuan, Kai Ji, Xiao-Jun Ren, Lu-Jing Zhang, Sen Wen, Jian-Ping Huang, He Front Microbiol Microbiology Pneumocandin B(0) is an important antifungal drug precursor produced by filamentous fungus Glarea lozoyensis. The high broth viscosity of cultures of this organism results in lower oxygen solubility and higher energy consumption for agitation and aeration, which mostly caused by the morphologies of filamentous fungi in submerged culture. In this study, the effects of different seed medium nitrogen sources on morphology and fermentation behavior of G. lozoyensis were investigated, and cotton seed powder resulted in small, compact pellets. Moreover, pneumocandin B(0) yield in Erlenmeyer flasks were increased by 22.9%. Furthermore, pneumocandin B(0) yield in a 50-L fermenter reached 2,100 mg/L and the dissolved oxygen maintained above 30%. Additionally, activities of phosphofructokinase (PFK), isocitrate dehydrogenase (ICDH), glucose 6-phosphate dehydrogenase (G6PDH), and malic enzyme (ME) were increased by 87.5, 50, 41.6, and 10.7%, respectively. This study demonstrates the feasibility and advantages of using cotton seed powder for controlling the fungal morphology and improving the product yield in pneumocandin fermentations. Frontiers Media S.A. 2018-10-10 /pmc/articles/PMC6191469/ /pubmed/30364147 http://dx.doi.org/10.3389/fmicb.2018.02352 Text en Copyright © 2018 Song, Yuan, Ji, Ren, Zhang, Wen and Huang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Song, Ping Yuan, Kai Ji, Xiao-Jun Ren, Lu-Jing Zhang, Sen Wen, Jian-Ping Huang, He Effects of Cotton Seed Powder as the Seed Medium Nitrogen Source on the Morphology and Pneumocandin B(0) Yield of Glarea lozoyensis |
title | Effects of Cotton Seed Powder as the Seed Medium Nitrogen Source on the Morphology and Pneumocandin B(0) Yield of Glarea lozoyensis |
title_full | Effects of Cotton Seed Powder as the Seed Medium Nitrogen Source on the Morphology and Pneumocandin B(0) Yield of Glarea lozoyensis |
title_fullStr | Effects of Cotton Seed Powder as the Seed Medium Nitrogen Source on the Morphology and Pneumocandin B(0) Yield of Glarea lozoyensis |
title_full_unstemmed | Effects of Cotton Seed Powder as the Seed Medium Nitrogen Source on the Morphology and Pneumocandin B(0) Yield of Glarea lozoyensis |
title_short | Effects of Cotton Seed Powder as the Seed Medium Nitrogen Source on the Morphology and Pneumocandin B(0) Yield of Glarea lozoyensis |
title_sort | effects of cotton seed powder as the seed medium nitrogen source on the morphology and pneumocandin b(0) yield of glarea lozoyensis |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6191469/ https://www.ncbi.nlm.nih.gov/pubmed/30364147 http://dx.doi.org/10.3389/fmicb.2018.02352 |
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