Cargando…

Pasteurization Preserves IL-8 in Human Milk

Background: Pasteurized donor human milk is an alternative feeding when mothers' own milk is not available for premature infants. The effects of pasteurization on the host defense properties of human milk are unclear. We investigated the effects of Holder pasteurization on concentrations of ant...

Descripción completa

Detalles Bibliográficos
Autores principales: Giorgi, Marilyn V., Codipilly, Champa N., Potak, Debra, Heiman, Howard S., Schanler, Richard J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6191475/
https://www.ncbi.nlm.nih.gov/pubmed/30364085
http://dx.doi.org/10.3389/fped.2018.00281
_version_ 1783363722848239616
author Giorgi, Marilyn V.
Codipilly, Champa N.
Potak, Debra
Heiman, Howard S.
Schanler, Richard J.
author_facet Giorgi, Marilyn V.
Codipilly, Champa N.
Potak, Debra
Heiman, Howard S.
Schanler, Richard J.
author_sort Giorgi, Marilyn V.
collection PubMed
description Background: Pasteurized donor human milk is an alternative feeding when mothers' own milk is not available for premature infants. The effects of pasteurization on the host defense properties of human milk are unclear. We investigated the effects of Holder pasteurization on concentrations of anti-inflammatory and pro-inflammatory cytokines in human milk. Objective: To compare concentrations of anti-inflammatory and pro-inflammatory cytokines before and after pasteurization of donor human milk. Study Design: A single milk sample was obtained from each of 24 mothers of premature infants in the neonatal intensive care unit by electric breast pump and was stored at −80°C. At the time of pasteurization, milk samples were thawed and divided into two aliquots. The first aliquot was re-stored at −80°C and the second aliquot was heat-treated at 62.5°C for 30 min and then re-stored at −80°C. At the time of batch cytokine analyses samples were thawed rapidly. Results: Most cytokine concentrations declined following pasteurization. The most prevalent cytokine, IL-8, was preserved (89%) following pasteurization. There were no relationships between gestational age, postnatal age of milk collection, duration of milk storage, and the concentrations cytokines. Conclusion: In contrast to most cytokines after pasteurization, IL-8 is preserved or liberated from another compartment. The maintenance of IL-8 in human milk after pasteurization and the loss of anti-inflammatory cytokines following pasteurization, suggests that the effects of inflammatory activity in pasteurized human milk should be evaluated. These data may account, in part, for the lesser protective effect on the host of pasteurized donor human milk compared with mother's own milk.
format Online
Article
Text
id pubmed-6191475
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-61914752018-10-24 Pasteurization Preserves IL-8 in Human Milk Giorgi, Marilyn V. Codipilly, Champa N. Potak, Debra Heiman, Howard S. Schanler, Richard J. Front Pediatr Pediatrics Background: Pasteurized donor human milk is an alternative feeding when mothers' own milk is not available for premature infants. The effects of pasteurization on the host defense properties of human milk are unclear. We investigated the effects of Holder pasteurization on concentrations of anti-inflammatory and pro-inflammatory cytokines in human milk. Objective: To compare concentrations of anti-inflammatory and pro-inflammatory cytokines before and after pasteurization of donor human milk. Study Design: A single milk sample was obtained from each of 24 mothers of premature infants in the neonatal intensive care unit by electric breast pump and was stored at −80°C. At the time of pasteurization, milk samples were thawed and divided into two aliquots. The first aliquot was re-stored at −80°C and the second aliquot was heat-treated at 62.5°C for 30 min and then re-stored at −80°C. At the time of batch cytokine analyses samples were thawed rapidly. Results: Most cytokine concentrations declined following pasteurization. The most prevalent cytokine, IL-8, was preserved (89%) following pasteurization. There were no relationships between gestational age, postnatal age of milk collection, duration of milk storage, and the concentrations cytokines. Conclusion: In contrast to most cytokines after pasteurization, IL-8 is preserved or liberated from another compartment. The maintenance of IL-8 in human milk after pasteurization and the loss of anti-inflammatory cytokines following pasteurization, suggests that the effects of inflammatory activity in pasteurized human milk should be evaluated. These data may account, in part, for the lesser protective effect on the host of pasteurized donor human milk compared with mother's own milk. Frontiers Media S.A. 2018-10-10 /pmc/articles/PMC6191475/ /pubmed/30364085 http://dx.doi.org/10.3389/fped.2018.00281 Text en Copyright © 2018 Giorgi, Codipilly, Potak, Heiman and Schanler. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pediatrics
Giorgi, Marilyn V.
Codipilly, Champa N.
Potak, Debra
Heiman, Howard S.
Schanler, Richard J.
Pasteurization Preserves IL-8 in Human Milk
title Pasteurization Preserves IL-8 in Human Milk
title_full Pasteurization Preserves IL-8 in Human Milk
title_fullStr Pasteurization Preserves IL-8 in Human Milk
title_full_unstemmed Pasteurization Preserves IL-8 in Human Milk
title_short Pasteurization Preserves IL-8 in Human Milk
title_sort pasteurization preserves il-8 in human milk
topic Pediatrics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6191475/
https://www.ncbi.nlm.nih.gov/pubmed/30364085
http://dx.doi.org/10.3389/fped.2018.00281
work_keys_str_mv AT giorgimarilynv pasteurizationpreservesil8inhumanmilk
AT codipillychampan pasteurizationpreservesil8inhumanmilk
AT potakdebra pasteurizationpreservesil8inhumanmilk
AT heimanhowards pasteurizationpreservesil8inhumanmilk
AT schanlerrichardj pasteurizationpreservesil8inhumanmilk