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Simplified immobilisation method for histidine-tagged enzymes in poly(methyl methacrylate) microfluidic devices

Poly(methyl methacrylate) (PMMA) microfluidic devices have become promising platforms for a wide range of applications. Here we report a simple method for immobilising histidine-tagged enzymes suitable for PMMA microfluidic devices. The 1-step-immobilisation described is based on the affinity of the...

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Detalles Bibliográficos
Autores principales:	Kulsharova, Gulsim, Dimov, Nikolay, Marques, Marco P.C., Szita, Nicolas, Baganz, Frank
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6191535/ https://www.ncbi.nlm.nih.gov/pubmed/29242048 http://dx.doi.org/10.1016/j.nbt.2017.12.004

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