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Rat BodyMap transcriptomes reveal unique circular RNA features across tissue types and developmental stages

Circular RNAs (circRNAs) are a novel class of regulatory RNAs. Here, we present a comprehensive investigation of circRNA expression profiles across 11 tissues and four developmental stages in rats, along with cross-species analyses in humans and mice. Although the expression of circRNAs is positivel...

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Detalles Bibliográficos
Autores principales: Zhou, Tong, Xie, Xueying, Li, Musheng, Shi, Junchao, Zhou, Jin J., Knox, Kenneth S., Wang, Ting, Chen, Qi, Gu, Wanjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6191709/
https://www.ncbi.nlm.nih.gov/pubmed/30093490
http://dx.doi.org/10.1261/rna.067132.118
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author Zhou, Tong
Xie, Xueying
Li, Musheng
Shi, Junchao
Zhou, Jin J.
Knox, Kenneth S.
Wang, Ting
Chen, Qi
Gu, Wanjun
author_facet Zhou, Tong
Xie, Xueying
Li, Musheng
Shi, Junchao
Zhou, Jin J.
Knox, Kenneth S.
Wang, Ting
Chen, Qi
Gu, Wanjun
author_sort Zhou, Tong
collection PubMed
description Circular RNAs (circRNAs) are a novel class of regulatory RNAs. Here, we present a comprehensive investigation of circRNA expression profiles across 11 tissues and four developmental stages in rats, along with cross-species analyses in humans and mice. Although the expression of circRNAs is positively correlated with that of cognate mRNAs, highly expressed genes tend to splice a larger fraction of circular transcripts. Moreover, circRNAs exhibit higher tissue specificity than cognate mRNAs. Intriguingly, while we observed a monotonic increase of circRNA abundance with age in the rat brain, we further discovered a dynamic, age-dependent pattern of circRNA expression in the testes that is characterized by a dramatic increase with advancing stages of sexual maturity and a decrease with aging. The age-sensitive testicular circRNAs are highly associated with spermatogenesis, independent of cognate mRNA expression. The tissue/age implications of circRNAs suggest that they present unique physiological functions rather than simply occurring as occasional by-products of gene transcription.
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spelling pubmed-61917092018-11-02 Rat BodyMap transcriptomes reveal unique circular RNA features across tissue types and developmental stages Zhou, Tong Xie, Xueying Li, Musheng Shi, Junchao Zhou, Jin J. Knox, Kenneth S. Wang, Ting Chen, Qi Gu, Wanjun RNA Bioinformatics Circular RNAs (circRNAs) are a novel class of regulatory RNAs. Here, we present a comprehensive investigation of circRNA expression profiles across 11 tissues and four developmental stages in rats, along with cross-species analyses in humans and mice. Although the expression of circRNAs is positively correlated with that of cognate mRNAs, highly expressed genes tend to splice a larger fraction of circular transcripts. Moreover, circRNAs exhibit higher tissue specificity than cognate mRNAs. Intriguingly, while we observed a monotonic increase of circRNA abundance with age in the rat brain, we further discovered a dynamic, age-dependent pattern of circRNA expression in the testes that is characterized by a dramatic increase with advancing stages of sexual maturity and a decrease with aging. The age-sensitive testicular circRNAs are highly associated with spermatogenesis, independent of cognate mRNA expression. The tissue/age implications of circRNAs suggest that they present unique physiological functions rather than simply occurring as occasional by-products of gene transcription. Cold Spring Harbor Laboratory Press 2018-11 /pmc/articles/PMC6191709/ /pubmed/30093490 http://dx.doi.org/10.1261/rna.067132.118 Text en © 2018 Zhou et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by/4.0/ This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.
spellingShingle Bioinformatics
Zhou, Tong
Xie, Xueying
Li, Musheng
Shi, Junchao
Zhou, Jin J.
Knox, Kenneth S.
Wang, Ting
Chen, Qi
Gu, Wanjun
Rat BodyMap transcriptomes reveal unique circular RNA features across tissue types and developmental stages
title Rat BodyMap transcriptomes reveal unique circular RNA features across tissue types and developmental stages
title_full Rat BodyMap transcriptomes reveal unique circular RNA features across tissue types and developmental stages
title_fullStr Rat BodyMap transcriptomes reveal unique circular RNA features across tissue types and developmental stages
title_full_unstemmed Rat BodyMap transcriptomes reveal unique circular RNA features across tissue types and developmental stages
title_short Rat BodyMap transcriptomes reveal unique circular RNA features across tissue types and developmental stages
title_sort rat bodymap transcriptomes reveal unique circular rna features across tissue types and developmental stages
topic Bioinformatics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6191709/
https://www.ncbi.nlm.nih.gov/pubmed/30093490
http://dx.doi.org/10.1261/rna.067132.118
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