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Combinatorial interplay of RNA-binding proteins tunes levels of mitochondrial mRNA in trypanosomes

MRP1/2 is a heteromeric protein complex that functions in the trypanosomatid mitochondrion as part of the RNA editing machinery, which facilitates multiple targeted insertions and deletions of uridines. MRP1/2 was shown to interact with MRB8170, which initiates RNA editing by marking pre-edited mRNA...

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Detalles Bibliográficos
Autores principales: Dixit, Sameer, Lukeš, Julius
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6191715/
https://www.ncbi.nlm.nih.gov/pubmed/30120147
http://dx.doi.org/10.1261/rna.066233.118
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author Dixit, Sameer
Lukeš, Julius
author_facet Dixit, Sameer
Lukeš, Julius
author_sort Dixit, Sameer
collection PubMed
description MRP1/2 is a heteromeric protein complex that functions in the trypanosomatid mitochondrion as part of the RNA editing machinery, which facilitates multiple targeted insertions and deletions of uridines. MRP1/2 was shown to interact with MRB8170, which initiates RNA editing by marking pre-edited mRNAs, while TbRGG2 is required for its efficient progression on pan-edited mRNAs. Both MRP1/2 and TbRGG2 are capable of modulating RNA–RNA interactions in vitro. As determined by using iCLIP and RIP-qPCR, RNAs bound to MRP1/2 are characterized and compared with those associated with MRB8170 and TbRGG2. We provide evidence that MRP1 and MRB8170 have correlated binding and similar RNA crosslinking peak profiles over minimally and never-edited mRNAs. Our results suggest that MRP1 assists MRB8170 in RNA editing on minimally edited mRNAs.
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spelling pubmed-61917152019-11-01 Combinatorial interplay of RNA-binding proteins tunes levels of mitochondrial mRNA in trypanosomes Dixit, Sameer Lukeš, Julius RNA Article MRP1/2 is a heteromeric protein complex that functions in the trypanosomatid mitochondrion as part of the RNA editing machinery, which facilitates multiple targeted insertions and deletions of uridines. MRP1/2 was shown to interact with MRB8170, which initiates RNA editing by marking pre-edited mRNAs, while TbRGG2 is required for its efficient progression on pan-edited mRNAs. Both MRP1/2 and TbRGG2 are capable of modulating RNA–RNA interactions in vitro. As determined by using iCLIP and RIP-qPCR, RNAs bound to MRP1/2 are characterized and compared with those associated with MRB8170 and TbRGG2. We provide evidence that MRP1 and MRB8170 have correlated binding and similar RNA crosslinking peak profiles over minimally and never-edited mRNAs. Our results suggest that MRP1 assists MRB8170 in RNA editing on minimally edited mRNAs. Cold Spring Harbor Laboratory Press 2018-11 /pmc/articles/PMC6191715/ /pubmed/30120147 http://dx.doi.org/10.1261/rna.066233.118 Text en © 2018 Dixit and Lukeš; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Article
Dixit, Sameer
Lukeš, Julius
Combinatorial interplay of RNA-binding proteins tunes levels of mitochondrial mRNA in trypanosomes
title Combinatorial interplay of RNA-binding proteins tunes levels of mitochondrial mRNA in trypanosomes
title_full Combinatorial interplay of RNA-binding proteins tunes levels of mitochondrial mRNA in trypanosomes
title_fullStr Combinatorial interplay of RNA-binding proteins tunes levels of mitochondrial mRNA in trypanosomes
title_full_unstemmed Combinatorial interplay of RNA-binding proteins tunes levels of mitochondrial mRNA in trypanosomes
title_short Combinatorial interplay of RNA-binding proteins tunes levels of mitochondrial mRNA in trypanosomes
title_sort combinatorial interplay of rna-binding proteins tunes levels of mitochondrial mrna in trypanosomes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6191715/
https://www.ncbi.nlm.nih.gov/pubmed/30120147
http://dx.doi.org/10.1261/rna.066233.118
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