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Reconstitution of 30S ribosomal subunits in vitro using ribosome biogenesis factors

Reconstitution of ribosomes in vitro from individual ribosomal proteins provides a powerful tool for understanding the ribosome assembly process including the sequential incorporation of ribosomal proteins. However, conventional assembly methods require high-salt conditions for efficient ribosome as...

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Detalles Bibliográficos
Autores principales: Tamaru, Daichi, Amikura, Kazuaki, Shimizu, Yoshihiro, Nierhaus, Knud H., Ueda, Takuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6191716/
https://www.ncbi.nlm.nih.gov/pubmed/30076205
http://dx.doi.org/10.1261/rna.065615.118