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Reconstitution of 30S ribosomal subunits in vitro using ribosome biogenesis factors
Reconstitution of ribosomes in vitro from individual ribosomal proteins provides a powerful tool for understanding the ribosome assembly process including the sequential incorporation of ribosomal proteins. However, conventional assembly methods require high-salt conditions for efficient ribosome as...
Autores principales: | Tamaru, Daichi, Amikura, Kazuaki, Shimizu, Yoshihiro, Nierhaus, Knud H., Ueda, Takuya |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6191716/ https://www.ncbi.nlm.nih.gov/pubmed/30076205 http://dx.doi.org/10.1261/rna.065615.118 |
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