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Developing specific molecular biomarkers for thermal stress in salmonids

BACKGROUND: Pacific salmon (Oncorhynchus spp.) serve as good biological indicators of the breadth of climate warming effects on fish because their anadromous life cycle exposes them to environmental challenges in both marine and freshwater environments. Our study sought to mine the extensive functio...

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Autores principales: Akbarzadeh, Arash, Günther, Oliver P, Houde, Aimee Lee, Li, Shaorong, Ming, Tobi J, Jeffries, Kenneth M, Hinch, Scott G, Miller, Kristina M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6192343/
https://www.ncbi.nlm.nih.gov/pubmed/30326831
http://dx.doi.org/10.1186/s12864-018-5108-9
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author Akbarzadeh, Arash
Günther, Oliver P
Houde, Aimee Lee
Li, Shaorong
Ming, Tobi J
Jeffries, Kenneth M
Hinch, Scott G
Miller, Kristina M
author_facet Akbarzadeh, Arash
Günther, Oliver P
Houde, Aimee Lee
Li, Shaorong
Ming, Tobi J
Jeffries, Kenneth M
Hinch, Scott G
Miller, Kristina M
author_sort Akbarzadeh, Arash
collection PubMed
description BACKGROUND: Pacific salmon (Oncorhynchus spp.) serve as good biological indicators of the breadth of climate warming effects on fish because their anadromous life cycle exposes them to environmental challenges in both marine and freshwater environments. Our study sought to mine the extensive functional genomic studies in fishes to identify robust thermally-responsive biomarkers that could monitor molecular physiological signatures of chronic thermal stress in fish using non-lethal sampling of gill tissue. RESULTS: Candidate thermal stress biomarkers for gill tissue were identified using comparisons among microarray datasets produced in the Molecular Genetics Laboratory, Pacific Biological Station, Nanaimo, BC, six external, published microarray studies on chronic and acute temperature stress in salmon, and a comparison of significant genes across published studies in multiple fishes using deep literature mining. Eighty-two microarray features related to 39 unique gene IDs were selected as candidate chronic thermal stress biomarkers. Most of these genes were identified both in the meta-analysis of salmon microarray data and in the literature mining for thermal stress markers in salmonids and other fishes. Quantitative reverse transcription PCR (qRT-PCR) assays for 32 unique genes with good efficiencies across salmon species were developed, and their activity in response to thermally challenged sockeye salmon (O. nerka) and Chinook salmon (O. tshawytscha) (cool, 13–14 °C and warm temperatures 18–19 °C) over 5–7 days was assessed. Eight genes, including two transcripts of each SERPINH1 and HSP90AA1, FKBP10, MAP3K14, SFRS2, and EEF2 showed strong and robust chronic temperature stress response consistently in the discovery analysis and both sockeye and Chinook salmon validation studies. CONCLUSIONS: The results of both discovery analysis and gene expression showed that a panel of genes involved in chaperoning and protein rescue, oxidative stress, and protein biosynthesis were differentially activated in gill tissue of Pacific salmon in response to elevated temperatures. While individually, some of these biomarkers may also respond to other stressors or biological processes, when expressed in concert, we argue that a biomarker panel comprised of some or all of these genes could provide a reliable means to specifically detect thermal stress in field-caught salmon. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-5108-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-61923432018-10-22 Developing specific molecular biomarkers for thermal stress in salmonids Akbarzadeh, Arash Günther, Oliver P Houde, Aimee Lee Li, Shaorong Ming, Tobi J Jeffries, Kenneth M Hinch, Scott G Miller, Kristina M BMC Genomics Research Article BACKGROUND: Pacific salmon (Oncorhynchus spp.) serve as good biological indicators of the breadth of climate warming effects on fish because their anadromous life cycle exposes them to environmental challenges in both marine and freshwater environments. Our study sought to mine the extensive functional genomic studies in fishes to identify robust thermally-responsive biomarkers that could monitor molecular physiological signatures of chronic thermal stress in fish using non-lethal sampling of gill tissue. RESULTS: Candidate thermal stress biomarkers for gill tissue were identified using comparisons among microarray datasets produced in the Molecular Genetics Laboratory, Pacific Biological Station, Nanaimo, BC, six external, published microarray studies on chronic and acute temperature stress in salmon, and a comparison of significant genes across published studies in multiple fishes using deep literature mining. Eighty-two microarray features related to 39 unique gene IDs were selected as candidate chronic thermal stress biomarkers. Most of these genes were identified both in the meta-analysis of salmon microarray data and in the literature mining for thermal stress markers in salmonids and other fishes. Quantitative reverse transcription PCR (qRT-PCR) assays for 32 unique genes with good efficiencies across salmon species were developed, and their activity in response to thermally challenged sockeye salmon (O. nerka) and Chinook salmon (O. tshawytscha) (cool, 13–14 °C and warm temperatures 18–19 °C) over 5–7 days was assessed. Eight genes, including two transcripts of each SERPINH1 and HSP90AA1, FKBP10, MAP3K14, SFRS2, and EEF2 showed strong and robust chronic temperature stress response consistently in the discovery analysis and both sockeye and Chinook salmon validation studies. CONCLUSIONS: The results of both discovery analysis and gene expression showed that a panel of genes involved in chaperoning and protein rescue, oxidative stress, and protein biosynthesis were differentially activated in gill tissue of Pacific salmon in response to elevated temperatures. While individually, some of these biomarkers may also respond to other stressors or biological processes, when expressed in concert, we argue that a biomarker panel comprised of some or all of these genes could provide a reliable means to specifically detect thermal stress in field-caught salmon. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-5108-9) contains supplementary material, which is available to authorized users. BioMed Central 2018-10-16 /pmc/articles/PMC6192343/ /pubmed/30326831 http://dx.doi.org/10.1186/s12864-018-5108-9 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Akbarzadeh, Arash
Günther, Oliver P
Houde, Aimee Lee
Li, Shaorong
Ming, Tobi J
Jeffries, Kenneth M
Hinch, Scott G
Miller, Kristina M
Developing specific molecular biomarkers for thermal stress in salmonids
title Developing specific molecular biomarkers for thermal stress in salmonids
title_full Developing specific molecular biomarkers for thermal stress in salmonids
title_fullStr Developing specific molecular biomarkers for thermal stress in salmonids
title_full_unstemmed Developing specific molecular biomarkers for thermal stress in salmonids
title_short Developing specific molecular biomarkers for thermal stress in salmonids
title_sort developing specific molecular biomarkers for thermal stress in salmonids
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6192343/
https://www.ncbi.nlm.nih.gov/pubmed/30326831
http://dx.doi.org/10.1186/s12864-018-5108-9
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