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Chemical and molecular characterization of metabolites from Flavobacterium sp.
In this study, a Flavobacterium sp. is isolated from natural spring, and identified using molecular techniques. Extracellular and intracellular secondary metabolites are identified using solid phase microextraction gas chromatography-mass spectrometry and ultra performance liquid chromatography. Cyt...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6192653/ https://www.ncbi.nlm.nih.gov/pubmed/30332474 http://dx.doi.org/10.1371/journal.pone.0205817 |
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author | Enisoglu-Atalay, Vildan Atasever-Arslan, Belkis Yaman, Bugra Cebecioglu, Rumeysa Kul, Aykut Ozilhan, Selma Ozen, Fatih Catal, Tunc |
author_facet | Enisoglu-Atalay, Vildan Atasever-Arslan, Belkis Yaman, Bugra Cebecioglu, Rumeysa Kul, Aykut Ozilhan, Selma Ozen, Fatih Catal, Tunc |
author_sort | Enisoglu-Atalay, Vildan |
collection | PubMed |
description | In this study, a Flavobacterium sp. is isolated from natural spring, and identified using molecular techniques. Extracellular and intracellular secondary metabolites are identified using solid phase microextraction gas chromatography-mass spectrometry and ultra performance liquid chromatography. Cytotoxic activity of the extracellular compounds produced by the Flavobacterium sp. and quercetin as the standard are measured using ECV304 human endothelial cells in vitro. Our results show that Flavobacterim sp. isolate has the highest percentage of similarity with Flavobacterium cheonhonense strain ARSA-15 (99%). Quercetin is detected as the major extracellular compound produced by the Flavobacterium sp. Methanol extract of Flavobacterium sp. resulted in a higher cell viability results when compared to DMSO extracts. Computational chemistry approach was used and it has been found that polar solvent (methanol) contributed to higher antioxidant activity. In conclusion, Flavobacterium sp. can be used to produce quercetin for industrial purposes. |
format | Online Article Text |
id | pubmed-6192653 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-61926532018-11-05 Chemical and molecular characterization of metabolites from Flavobacterium sp. Enisoglu-Atalay, Vildan Atasever-Arslan, Belkis Yaman, Bugra Cebecioglu, Rumeysa Kul, Aykut Ozilhan, Selma Ozen, Fatih Catal, Tunc PLoS One Research Article In this study, a Flavobacterium sp. is isolated from natural spring, and identified using molecular techniques. Extracellular and intracellular secondary metabolites are identified using solid phase microextraction gas chromatography-mass spectrometry and ultra performance liquid chromatography. Cytotoxic activity of the extracellular compounds produced by the Flavobacterium sp. and quercetin as the standard are measured using ECV304 human endothelial cells in vitro. Our results show that Flavobacterim sp. isolate has the highest percentage of similarity with Flavobacterium cheonhonense strain ARSA-15 (99%). Quercetin is detected as the major extracellular compound produced by the Flavobacterium sp. Methanol extract of Flavobacterium sp. resulted in a higher cell viability results when compared to DMSO extracts. Computational chemistry approach was used and it has been found that polar solvent (methanol) contributed to higher antioxidant activity. In conclusion, Flavobacterium sp. can be used to produce quercetin for industrial purposes. Public Library of Science 2018-10-17 /pmc/articles/PMC6192653/ /pubmed/30332474 http://dx.doi.org/10.1371/journal.pone.0205817 Text en © 2018 Enisoglu-Atalay et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Enisoglu-Atalay, Vildan Atasever-Arslan, Belkis Yaman, Bugra Cebecioglu, Rumeysa Kul, Aykut Ozilhan, Selma Ozen, Fatih Catal, Tunc Chemical and molecular characterization of metabolites from Flavobacterium sp. |
title | Chemical and molecular characterization of metabolites from Flavobacterium sp. |
title_full | Chemical and molecular characterization of metabolites from Flavobacterium sp. |
title_fullStr | Chemical and molecular characterization of metabolites from Flavobacterium sp. |
title_full_unstemmed | Chemical and molecular characterization of metabolites from Flavobacterium sp. |
title_short | Chemical and molecular characterization of metabolites from Flavobacterium sp. |
title_sort | chemical and molecular characterization of metabolites from flavobacterium sp. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6192653/ https://www.ncbi.nlm.nih.gov/pubmed/30332474 http://dx.doi.org/10.1371/journal.pone.0205817 |
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