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Potential anti-vitiligo properties of cynarine extracted from Vernonia anthelmintica (L.) Willd
Vitiligo is a depigmentation disorder of the skin. It is primarily caused by the destruction of melanocytes or obstruction of the melanin synthesis pathway. Melanin is a type of skin pigment that determines skin color. The seeds of Vernonia anthelmintica (L.) Willd (Kaliziri) are used for treating s...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6192770/ https://www.ncbi.nlm.nih.gov/pubmed/30226537 http://dx.doi.org/10.3892/ijmm.2018.3861 |
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author | Mamat, Nuramina Lu, Xue Ying Kabas, Maidina Aisa, Haji Akber |
author_facet | Mamat, Nuramina Lu, Xue Ying Kabas, Maidina Aisa, Haji Akber |
author_sort | Mamat, Nuramina |
collection | PubMed |
description | Vitiligo is a depigmentation disorder of the skin. It is primarily caused by the destruction of melanocytes or obstruction of the melanin synthesis pathway. Melanin is a type of skin pigment that determines skin color. The seeds of Vernonia anthelmintica (L.) Willd (Kaliziri) are used for treating skin diseases including vitiligo in traditional Uyghur medicine. 1,5-Dicaffeoylquinic acid (1,5-diCQA) is a natural polyphenolic compound widely distributed in plants and extracted from Kaliziri seeds. Therefore, in the present study, the effect of 1,5-diCQA on melanin synthesis in B16 cell was evaluated, and its molecular mechanism was explored. The results indicated that 1,5-diCQA treatment of B16 cells stimulated an increase of intracellular melanin level and tyrosinase (TYR) activity without cytotoxicity. Reverse transcription quantitative polymerase chain reaction results also indicated that 1,5-diCQA may markedly improve the protein expression and RNA transcription of microphthalmia-associated transcription factor (MITF), melanogenic enzyme Tyr, tyrosinase-related protein 1 (TRP 1) and tyrosinase-related protein 2 (TRP 2). Additional results identified that 1,5-diCQA may promote the phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK) and extracellular signal-regulated kinase (ERK) MAPK. Notably, the increased levels of intracellular melanin synthesis and tyrosinase expression induced by 1,5-diCQA treatment were significantly attenuated by the protein kinase A (PKA) inhibitor H-89. Intracellular cyclic adenosine monophosphate (cAMP) concentration and phosphorylation of cAMP-response element binding protein was increased following 1,5-diCQA treatment. These results indicated that 1,5-diCQA stimulated melanogenesis via the MAPK and cAMP/PKA signaling pathways in B16 cells, which has potential therapeutic implications for vitiligo. |
format | Online Article Text |
id | pubmed-6192770 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-61927702018-10-22 Potential anti-vitiligo properties of cynarine extracted from Vernonia anthelmintica (L.) Willd Mamat, Nuramina Lu, Xue Ying Kabas, Maidina Aisa, Haji Akber Int J Mol Med Articles Vitiligo is a depigmentation disorder of the skin. It is primarily caused by the destruction of melanocytes or obstruction of the melanin synthesis pathway. Melanin is a type of skin pigment that determines skin color. The seeds of Vernonia anthelmintica (L.) Willd (Kaliziri) are used for treating skin diseases including vitiligo in traditional Uyghur medicine. 1,5-Dicaffeoylquinic acid (1,5-diCQA) is a natural polyphenolic compound widely distributed in plants and extracted from Kaliziri seeds. Therefore, in the present study, the effect of 1,5-diCQA on melanin synthesis in B16 cell was evaluated, and its molecular mechanism was explored. The results indicated that 1,5-diCQA treatment of B16 cells stimulated an increase of intracellular melanin level and tyrosinase (TYR) activity without cytotoxicity. Reverse transcription quantitative polymerase chain reaction results also indicated that 1,5-diCQA may markedly improve the protein expression and RNA transcription of microphthalmia-associated transcription factor (MITF), melanogenic enzyme Tyr, tyrosinase-related protein 1 (TRP 1) and tyrosinase-related protein 2 (TRP 2). Additional results identified that 1,5-diCQA may promote the phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK) and extracellular signal-regulated kinase (ERK) MAPK. Notably, the increased levels of intracellular melanin synthesis and tyrosinase expression induced by 1,5-diCQA treatment were significantly attenuated by the protein kinase A (PKA) inhibitor H-89. Intracellular cyclic adenosine monophosphate (cAMP) concentration and phosphorylation of cAMP-response element binding protein was increased following 1,5-diCQA treatment. These results indicated that 1,5-diCQA stimulated melanogenesis via the MAPK and cAMP/PKA signaling pathways in B16 cells, which has potential therapeutic implications for vitiligo. D.A. Spandidos 2018-11 2018-09-06 /pmc/articles/PMC6192770/ /pubmed/30226537 http://dx.doi.org/10.3892/ijmm.2018.3861 Text en Copyright: © Mamat et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Mamat, Nuramina Lu, Xue Ying Kabas, Maidina Aisa, Haji Akber Potential anti-vitiligo properties of cynarine extracted from Vernonia anthelmintica (L.) Willd |
title | Potential anti-vitiligo properties of cynarine extracted from Vernonia anthelmintica (L.) Willd |
title_full | Potential anti-vitiligo properties of cynarine extracted from Vernonia anthelmintica (L.) Willd |
title_fullStr | Potential anti-vitiligo properties of cynarine extracted from Vernonia anthelmintica (L.) Willd |
title_full_unstemmed | Potential anti-vitiligo properties of cynarine extracted from Vernonia anthelmintica (L.) Willd |
title_short | Potential anti-vitiligo properties of cynarine extracted from Vernonia anthelmintica (L.) Willd |
title_sort | potential anti-vitiligo properties of cynarine extracted from vernonia anthelmintica (l.) willd |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6192770/ https://www.ncbi.nlm.nih.gov/pubmed/30226537 http://dx.doi.org/10.3892/ijmm.2018.3861 |
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