Cargando…

lncRNA LINC00673 induces proliferation, metastasis and epithelial-mesenchymal transition in thyroid carcinoma via Kruppel-like factor 2

The incidence of thyroid cancer has increased in the past decades; however, the underlying molecular mechanisms of thyroid cancer tumorigenesis remain unknown. Using sequencing technology, long intergenic non-protein coding RNA 673 (LINC00673) was identified to be upregulated in several tumor tissue...

Descripción completa

Detalles Bibliográficos
Autores principales: Xia, Erjie, Bhandari, Adheesh, Shen, Yanyan, Zhou, Xiaofen, Wang, Ouchen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6192783/
https://www.ncbi.nlm.nih.gov/pubmed/30106140
http://dx.doi.org/10.3892/ijo.2018.4524
_version_ 1783363961078415360
author Xia, Erjie
Bhandari, Adheesh
Shen, Yanyan
Zhou, Xiaofen
Wang, Ouchen
author_facet Xia, Erjie
Bhandari, Adheesh
Shen, Yanyan
Zhou, Xiaofen
Wang, Ouchen
author_sort Xia, Erjie
collection PubMed
description The incidence of thyroid cancer has increased in the past decades; however, the underlying molecular mechanisms of thyroid cancer tumorigenesis remain unknown. Using sequencing technology, long intergenic non-protein coding RNA 673 (LINC00673) was identified to be upregulated in several tumor tissues. However, the biological role of LINC00673 in thyroid carcinoma has yet to be determined. In this study, 60 matched pairs of thyroid tumor tissue and normal tissue were selected for study using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to validate previous findings; then, clinicopathologic features of the tissues were analyzed. Proliferation, colony formation, migration and invasion assays were performed, and epithelial-mesenchymal transition (EMT)- associated phenotypes were investigated following transfection with small interfering RNA to determine the specific role of LINC00673 in thyroid carcinoma cell lines (TPC1, KTC-1 and BCPAP). The study revealed that long non-coding RNA LINC00673 was significantly upregulated in thyroid cancer tissues compared with paired adjacent non-tumor tissues using RT-qPCR and that high expression of LINC00673 is was associated with larger tumor size and lymph node metastasis in the validated cohort. Knockdown of LINC00673 inhibited cell proliferation and metastasis, whereas, LINC00673 overexpression had the opposite effect. The results showed that LINC00673 may influence EMT and the expression of Kruppel-like factor 2 (KLF2). Notably, KLF2 is considered a tumor suppressor gene in a variety of tumors. Finally, knock down of KLF2 enhanced thyroid carcinoma cell proliferation, and invasion and migration. In this study, the function of LINC00673 in promoting the proliferation and metastasis of thyroid carcinoma cell lines was identified, and LINC00673 may act as a novel therapeutic target for treating thyroid carcinoma.
format Online
Article
Text
id pubmed-6192783
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-61927832018-10-22 lncRNA LINC00673 induces proliferation, metastasis and epithelial-mesenchymal transition in thyroid carcinoma via Kruppel-like factor 2 Xia, Erjie Bhandari, Adheesh Shen, Yanyan Zhou, Xiaofen Wang, Ouchen Int J Oncol Articles The incidence of thyroid cancer has increased in the past decades; however, the underlying molecular mechanisms of thyroid cancer tumorigenesis remain unknown. Using sequencing technology, long intergenic non-protein coding RNA 673 (LINC00673) was identified to be upregulated in several tumor tissues. However, the biological role of LINC00673 in thyroid carcinoma has yet to be determined. In this study, 60 matched pairs of thyroid tumor tissue and normal tissue were selected for study using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to validate previous findings; then, clinicopathologic features of the tissues were analyzed. Proliferation, colony formation, migration and invasion assays were performed, and epithelial-mesenchymal transition (EMT)- associated phenotypes were investigated following transfection with small interfering RNA to determine the specific role of LINC00673 in thyroid carcinoma cell lines (TPC1, KTC-1 and BCPAP). The study revealed that long non-coding RNA LINC00673 was significantly upregulated in thyroid cancer tissues compared with paired adjacent non-tumor tissues using RT-qPCR and that high expression of LINC00673 is was associated with larger tumor size and lymph node metastasis in the validated cohort. Knockdown of LINC00673 inhibited cell proliferation and metastasis, whereas, LINC00673 overexpression had the opposite effect. The results showed that LINC00673 may influence EMT and the expression of Kruppel-like factor 2 (KLF2). Notably, KLF2 is considered a tumor suppressor gene in a variety of tumors. Finally, knock down of KLF2 enhanced thyroid carcinoma cell proliferation, and invasion and migration. In this study, the function of LINC00673 in promoting the proliferation and metastasis of thyroid carcinoma cell lines was identified, and LINC00673 may act as a novel therapeutic target for treating thyroid carcinoma. D.A. Spandidos 2018-08-14 /pmc/articles/PMC6192783/ /pubmed/30106140 http://dx.doi.org/10.3892/ijo.2018.4524 Text en Copyright: © Xia et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Xia, Erjie
Bhandari, Adheesh
Shen, Yanyan
Zhou, Xiaofen
Wang, Ouchen
lncRNA LINC00673 induces proliferation, metastasis and epithelial-mesenchymal transition in thyroid carcinoma via Kruppel-like factor 2
title lncRNA LINC00673 induces proliferation, metastasis and epithelial-mesenchymal transition in thyroid carcinoma via Kruppel-like factor 2
title_full lncRNA LINC00673 induces proliferation, metastasis and epithelial-mesenchymal transition in thyroid carcinoma via Kruppel-like factor 2
title_fullStr lncRNA LINC00673 induces proliferation, metastasis and epithelial-mesenchymal transition in thyroid carcinoma via Kruppel-like factor 2
title_full_unstemmed lncRNA LINC00673 induces proliferation, metastasis and epithelial-mesenchymal transition in thyroid carcinoma via Kruppel-like factor 2
title_short lncRNA LINC00673 induces proliferation, metastasis and epithelial-mesenchymal transition in thyroid carcinoma via Kruppel-like factor 2
title_sort lncrna linc00673 induces proliferation, metastasis and epithelial-mesenchymal transition in thyroid carcinoma via kruppel-like factor 2
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6192783/
https://www.ncbi.nlm.nih.gov/pubmed/30106140
http://dx.doi.org/10.3892/ijo.2018.4524
work_keys_str_mv AT xiaerjie lncrnalinc00673inducesproliferationmetastasisandepithelialmesenchymaltransitioninthyroidcarcinomaviakruppellikefactor2
AT bhandariadheesh lncrnalinc00673inducesproliferationmetastasisandepithelialmesenchymaltransitioninthyroidcarcinomaviakruppellikefactor2
AT shenyanyan lncrnalinc00673inducesproliferationmetastasisandepithelialmesenchymaltransitioninthyroidcarcinomaviakruppellikefactor2
AT zhouxiaofen lncrnalinc00673inducesproliferationmetastasisandepithelialmesenchymaltransitioninthyroidcarcinomaviakruppellikefactor2
AT wangouchen lncrnalinc00673inducesproliferationmetastasisandepithelialmesenchymaltransitioninthyroidcarcinomaviakruppellikefactor2