CRISPR-induced exon skipping is dependent on premature termination codon mutations

In previous studies, CRISPR/Cas9 was shown to induce unexpected exon skipping; however, the mechanism by which this phenomenon is triggered is controversial. By analyzing 22 gene-edited rabbit lines generated using CRISPR/Cas9, we provide evidence of exon skipping at high frequency in premature term...

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Autores principales: Sui, Tingting, Song, Yuning, Liu, Zhiquan, Chen, Mao, Deng, Jichao, Xu, Yuanyuan, Lai, Liangxue, Li, Zhanjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6193291/
https://www.ncbi.nlm.nih.gov/pubmed/30333044
http://dx.doi.org/10.1186/s13059-018-1532-z
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author Sui, Tingting
Song, Yuning
Liu, Zhiquan
Chen, Mao
Deng, Jichao
Xu, Yuanyuan
Lai, Liangxue
Li, Zhanjun
author_facet Sui, Tingting
Song, Yuning
Liu, Zhiquan
Chen, Mao
Deng, Jichao
Xu, Yuanyuan
Lai, Liangxue
Li, Zhanjun
author_sort Sui, Tingting
collection PubMed
description In previous studies, CRISPR/Cas9 was shown to induce unexpected exon skipping; however, the mechanism by which this phenomenon is triggered is controversial. By analyzing 22 gene-edited rabbit lines generated using CRISPR/Cas9, we provide evidence of exon skipping at high frequency in premature termination codon-mutated rabbits but not in the rabbits with a premature termination codon mutation in exon 1 rabbits with non-frameshift or missense mutations. Our results suggest that CRISPR-mediated exon skipping depends on premature termination codon mutation-induced nonsense-associated altered splicing. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13059-018-1532-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-61932912018-10-22 CRISPR-induced exon skipping is dependent on premature termination codon mutations Sui, Tingting Song, Yuning Liu, Zhiquan Chen, Mao Deng, Jichao Xu, Yuanyuan Lai, Liangxue Li, Zhanjun Genome Biol Short Report In previous studies, CRISPR/Cas9 was shown to induce unexpected exon skipping; however, the mechanism by which this phenomenon is triggered is controversial. By analyzing 22 gene-edited rabbit lines generated using CRISPR/Cas9, we provide evidence of exon skipping at high frequency in premature termination codon-mutated rabbits but not in the rabbits with a premature termination codon mutation in exon 1 rabbits with non-frameshift or missense mutations. Our results suggest that CRISPR-mediated exon skipping depends on premature termination codon mutation-induced nonsense-associated altered splicing. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13059-018-1532-z) contains supplementary material, which is available to authorized users. BioMed Central 2018-10-17 /pmc/articles/PMC6193291/ /pubmed/30333044 http://dx.doi.org/10.1186/s13059-018-1532-z Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Sui, Tingting
Song, Yuning
Liu, Zhiquan
Chen, Mao
Deng, Jichao
Xu, Yuanyuan
Lai, Liangxue
Li, Zhanjun
CRISPR-induced exon skipping is dependent on premature termination codon mutations
title CRISPR-induced exon skipping is dependent on premature termination codon mutations
title_full CRISPR-induced exon skipping is dependent on premature termination codon mutations
title_fullStr CRISPR-induced exon skipping is dependent on premature termination codon mutations
title_full_unstemmed CRISPR-induced exon skipping is dependent on premature termination codon mutations
title_short CRISPR-induced exon skipping is dependent on premature termination codon mutations
title_sort crispr-induced exon skipping is dependent on premature termination codon mutations
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6193291/
https://www.ncbi.nlm.nih.gov/pubmed/30333044
http://dx.doi.org/10.1186/s13059-018-1532-z
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