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Fast and inexpensive protocols for consistent extraction of high quality DNA and RNA from challenging plant and fungal samples for high-throughput SNP genotyping and sequencing applications

Modern genotyping techniques, such as SNP analysis and genotyping by sequencing (GBS), are hampered by poor DNA quality and purity, particularly in challenging plant species, rich in secondary metabolites. We therefore investigated the utility of a pre-wash step using a buffered sorbitol solution, p...

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Detalles Bibliográficos
Autores principales:	Inglis, Peter W., Pappas, Marilia de Castro R., Resende, Lucileide V., Grattapaglia, Dario
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2018
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6193717/ https://www.ncbi.nlm.nih.gov/pubmed/30335843 http://dx.doi.org/10.1371/journal.pone.0206085

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