Crystallographic snapshots of ligand binding to hexameric purine nucleoside phosphorylase and kinetic studies give insight into the mechanism of catalysis

Purine nucleoside phosphorylase (PNP) catalyses the cleavage of the glycosidic bond of purine nucleosides using phosphate instead of water as a second substrate. PNP from Escherichia coli is a homohexamer, build as a trimer of dimers, and each subunit can be in two conformations, open or closed. Thi...

Descripción completa

Detalles Bibliográficos
Autores principales: Štefanić, Zoran, Narczyk, Marta, Mikleušević, Goran, Kazazić, Saša, Bzowska, Agnieszka, Luić, Marija
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6193948/
https://www.ncbi.nlm.nih.gov/pubmed/30337572
http://dx.doi.org/10.1038/s41598-018-33723-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6193948/
https://www.ncbi.nlm.nih.gov/pubmed/30337572
http://dx.doi.org/10.1038/s41598-018-33723-1

Ejemplares similares