Regulatory elements in vectors containing the ctEF-1α first intron and double enhancers for an efficient recombinant protein expression system

To establish a stable and scalable transient protein production system, we modified the EF-1 first intron size and verified the order of two recombinant enhancers downstream of the SV40 polyA sequence. This new vector was named pHH-Gemini (pHH-GM1) and was used to express alpha kinase 1 (ALPK1) and...

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Detalles Bibliográficos
Autores principales: Lee, Chi-Pin, Ko, Albert Min-Shan, Chiang, Shang-Lun, Lu, Chi-Yu, Tsai, Eing-Mei, Ko, Ying-Chin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6193983/
https://www.ncbi.nlm.nih.gov/pubmed/30337625
http://dx.doi.org/10.1038/s41598-018-33500-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6193983/
https://www.ncbi.nlm.nih.gov/pubmed/30337625
http://dx.doi.org/10.1038/s41598-018-33500-0

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