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The pathogenic mechanisms of Tilletia horrida as revealed by comparative and functional genomics
Tilletia horrida is a soil-borne, mononucleate basidiomycete fungus with a biotrophic lifestyle that causes rice kernel smut, a disease that is distributed throughout hybrid rice growing areas worldwide. Here we report on the high-quality genome sequence of T. horrida; it is composed of 23.2 Mb that...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6194002/ https://www.ncbi.nlm.nih.gov/pubmed/30337609 http://dx.doi.org/10.1038/s41598-018-33752-w |
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author | Wang, Aijun Pang, Linxiu Wang, Na Ai, Peng Yin, Desuo Li, Shuangcheng Deng, Qiming Zhu, Jun Liang, Yueyang Zhu, Jianqing Li, Ping Zheng, Aiping |
author_facet | Wang, Aijun Pang, Linxiu Wang, Na Ai, Peng Yin, Desuo Li, Shuangcheng Deng, Qiming Zhu, Jun Liang, Yueyang Zhu, Jianqing Li, Ping Zheng, Aiping |
author_sort | Wang, Aijun |
collection | PubMed |
description | Tilletia horrida is a soil-borne, mononucleate basidiomycete fungus with a biotrophic lifestyle that causes rice kernel smut, a disease that is distributed throughout hybrid rice growing areas worldwide. Here we report on the high-quality genome sequence of T. horrida; it is composed of 23.2 Mb that encode 7,729 predicted genes and 6,973 genes supported by RNA-seq. The genome contains few repetitive elements that account for 8.45% of the total. Evolutionarily, T. horrida lies close to the Ustilago fungi, suggesting grass species as potential hosts, but co-linearity was not observed between T. horrida and the barley smut Ustilago hordei. Genes and functions relevant to pathogenicity were presumed. T. horrida possesses a smaller set of carbohydrate-active enzymes and secondary metabolites, which probably reflect the specific characteristics of its infection and biotrophic lifestyle. Genes that encode secreted proteins and enzymes of secondary metabolism, and genes that are represented in the pathogen-host interaction gene database genes, are highly expressed during early infection; this is consistent with their potential roles in pathogenicity. Furthermore, among the 131 candidate pathogen effectors identified according to their expression patterns and functionality, we validated two that trigger leaf cell death in Nicotiana benthamiana. In summary, we have revealed new molecular mechanisms involved in the evolution, biotrophy, and pathogenesis of T. horrida. |
format | Online Article Text |
id | pubmed-6194002 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-61940022018-10-24 The pathogenic mechanisms of Tilletia horrida as revealed by comparative and functional genomics Wang, Aijun Pang, Linxiu Wang, Na Ai, Peng Yin, Desuo Li, Shuangcheng Deng, Qiming Zhu, Jun Liang, Yueyang Zhu, Jianqing Li, Ping Zheng, Aiping Sci Rep Article Tilletia horrida is a soil-borne, mononucleate basidiomycete fungus with a biotrophic lifestyle that causes rice kernel smut, a disease that is distributed throughout hybrid rice growing areas worldwide. Here we report on the high-quality genome sequence of T. horrida; it is composed of 23.2 Mb that encode 7,729 predicted genes and 6,973 genes supported by RNA-seq. The genome contains few repetitive elements that account for 8.45% of the total. Evolutionarily, T. horrida lies close to the Ustilago fungi, suggesting grass species as potential hosts, but co-linearity was not observed between T. horrida and the barley smut Ustilago hordei. Genes and functions relevant to pathogenicity were presumed. T. horrida possesses a smaller set of carbohydrate-active enzymes and secondary metabolites, which probably reflect the specific characteristics of its infection and biotrophic lifestyle. Genes that encode secreted proteins and enzymes of secondary metabolism, and genes that are represented in the pathogen-host interaction gene database genes, are highly expressed during early infection; this is consistent with their potential roles in pathogenicity. Furthermore, among the 131 candidate pathogen effectors identified according to their expression patterns and functionality, we validated two that trigger leaf cell death in Nicotiana benthamiana. In summary, we have revealed new molecular mechanisms involved in the evolution, biotrophy, and pathogenesis of T. horrida. Nature Publishing Group UK 2018-10-18 /pmc/articles/PMC6194002/ /pubmed/30337609 http://dx.doi.org/10.1038/s41598-018-33752-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Wang, Aijun Pang, Linxiu Wang, Na Ai, Peng Yin, Desuo Li, Shuangcheng Deng, Qiming Zhu, Jun Liang, Yueyang Zhu, Jianqing Li, Ping Zheng, Aiping The pathogenic mechanisms of Tilletia horrida as revealed by comparative and functional genomics |
title | The pathogenic mechanisms of Tilletia horrida as revealed by comparative and functional genomics |
title_full | The pathogenic mechanisms of Tilletia horrida as revealed by comparative and functional genomics |
title_fullStr | The pathogenic mechanisms of Tilletia horrida as revealed by comparative and functional genomics |
title_full_unstemmed | The pathogenic mechanisms of Tilletia horrida as revealed by comparative and functional genomics |
title_short | The pathogenic mechanisms of Tilletia horrida as revealed by comparative and functional genomics |
title_sort | pathogenic mechanisms of tilletia horrida as revealed by comparative and functional genomics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6194002/ https://www.ncbi.nlm.nih.gov/pubmed/30337609 http://dx.doi.org/10.1038/s41598-018-33752-w |
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