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Chemotaxis of Escherichia coli to major hormones and polyamines present in human gut
The microorganisms in the gastrointestinal (GI) tract can influence the metabolism, immunity, and behavior of animal hosts. Increasing evidence suggests that communication between the host and the microbiome also occurs in the opposite direction, with hormones and other host-secreted compounds being...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6194112/ https://www.ncbi.nlm.nih.gov/pubmed/29995838 http://dx.doi.org/10.1038/s41396-018-0227-5 |
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author | Lopes, Joana G. Sourjik, Victor |
author_facet | Lopes, Joana G. Sourjik, Victor |
author_sort | Lopes, Joana G. |
collection | PubMed |
description | The microorganisms in the gastrointestinal (GI) tract can influence the metabolism, immunity, and behavior of animal hosts. Increasing evidence suggests that communication between the host and the microbiome also occurs in the opposite direction, with hormones and other host-secreted compounds being sensed by microorganisms. Here, we addressed one key aspect of the host–microbe communication by studying chemotaxis of a model commensal bacterium, Escherichia coli, to several compounds present abundantly in the GI tract, namely catecholamines, thyroid hormones, and polyamines. Our results show that E. coli reacts to five out of ten analyzed chemicals, sensing melatonin, and spermidine as chemorepellents and showing mixed responses to dopamine, norepinephrine and 3,4-dihydroxymandelic acid. The strongest repellent response was observed for the polyamine spermidine, and we demonstrate that this response involves the low-abundance chemoreceptor Trg and the periplasmic binding protein PotD of the spermidine uptake system. The chemotactic effects of the tested compounds apparently correlate with their influence on growth and their stability in the GI tract, pointing to the specificity of the observed behavior. We hypothesize that the repellent responses observed at high concentrations of chemoeffective compounds might enable bacteria to avoid harmful levels of hormones and polyamines in the gut and, more generally, antimicrobial activities of the mucous layer. |
format | Online Article Text |
id | pubmed-6194112 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-61941122018-10-22 Chemotaxis of Escherichia coli to major hormones and polyamines present in human gut Lopes, Joana G. Sourjik, Victor ISME J Article The microorganisms in the gastrointestinal (GI) tract can influence the metabolism, immunity, and behavior of animal hosts. Increasing evidence suggests that communication between the host and the microbiome also occurs in the opposite direction, with hormones and other host-secreted compounds being sensed by microorganisms. Here, we addressed one key aspect of the host–microbe communication by studying chemotaxis of a model commensal bacterium, Escherichia coli, to several compounds present abundantly in the GI tract, namely catecholamines, thyroid hormones, and polyamines. Our results show that E. coli reacts to five out of ten analyzed chemicals, sensing melatonin, and spermidine as chemorepellents and showing mixed responses to dopamine, norepinephrine and 3,4-dihydroxymandelic acid. The strongest repellent response was observed for the polyamine spermidine, and we demonstrate that this response involves the low-abundance chemoreceptor Trg and the periplasmic binding protein PotD of the spermidine uptake system. The chemotactic effects of the tested compounds apparently correlate with their influence on growth and their stability in the GI tract, pointing to the specificity of the observed behavior. We hypothesize that the repellent responses observed at high concentrations of chemoeffective compounds might enable bacteria to avoid harmful levels of hormones and polyamines in the gut and, more generally, antimicrobial activities of the mucous layer. Nature Publishing Group UK 2018-07-11 2018-11 /pmc/articles/PMC6194112/ /pubmed/29995838 http://dx.doi.org/10.1038/s41396-018-0227-5 Text en © International Society for Microbial Ecology 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Lopes, Joana G. Sourjik, Victor Chemotaxis of Escherichia coli to major hormones and polyamines present in human gut |
title | Chemotaxis of Escherichia coli to major hormones and polyamines present in human gut |
title_full | Chemotaxis of Escherichia coli to major hormones and polyamines present in human gut |
title_fullStr | Chemotaxis of Escherichia coli to major hormones and polyamines present in human gut |
title_full_unstemmed | Chemotaxis of Escherichia coli to major hormones and polyamines present in human gut |
title_short | Chemotaxis of Escherichia coli to major hormones and polyamines present in human gut |
title_sort | chemotaxis of escherichia coli to major hormones and polyamines present in human gut |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6194112/ https://www.ncbi.nlm.nih.gov/pubmed/29995838 http://dx.doi.org/10.1038/s41396-018-0227-5 |
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